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Method for rapid screening of electronic cigarette liquid based on streptococcus mutans

A technology of Streptococcus mutans and electronic cigarette liquid, which is applied in the directions of biochemical equipment and methods, microbial determination/inspection, measurement device, etc. It can reduce the development cost and cycle, and achieve the effect of high screening efficiency.

Active Publication Date: 2018-08-21
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the aroma and smell of e-liquid smoke is quite different from that of traditional cigarettes, and the consumer acceptance is not high. Therefore, the e-liquid formula needs to be improved to meet the needs of consumers
At present, there is no public report on the antibacterial activity of e-liquids screened against different bacteria

Method used

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  • Method for rapid screening of electronic cigarette liquid based on streptococcus mutans
  • Method for rapid screening of electronic cigarette liquid based on streptococcus mutans
  • Method for rapid screening of electronic cigarette liquid based on streptococcus mutans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1 Materials and methods

[0033] 1.1 Test strain: Streptococcus mutans ( Streptococcus mutans , ATCC25175), purchased from Guangdong Microbial Culture Collection Center.

[0034] 1.2 Reagents and medium: PBS buffer (pH 7.2-7.4), HyClone, USA; 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT) , Sigma Corporation of the United States; dimethyl sulfoxide (DMSO), Xilong Chemical Co., Ltd.; kanamycin, Sangon Bioengineering (Shanghai) Co., Ltd.; nutrient broth, Hangzhou Microbial Reagent Co., Ltd.

[0035] 1.3 Equipment: Class II biological safety cabinet, Shanghai Likang Company, HFsafe-1500 type; microplate shaker; inverted microscope, Nikon Company, Japan, ECLIPSE TS100-F-HMC type; high-pressure steam sterilizer, ALP Company, Japan , CL-40M type; electronic balance, Swiss Mettler Toledo company, XS204 type, sensitivity 0.1mg; pH meter, Swiss Mettler Toledo company, Seven Esay S20K type; water-proof constant temperature incubator, Guangzhou Yongcheng compan...

Embodiment 2

[0060] Weigh 10.0g of the basic e-cigarette liquid sample, add tea extract according to the sensory evaluation results, the addition amount is 0.5g, the sample number is YY2; the control group is the basic e-cigarette liquid, the number is YY0.

[0061] Carry out antibacterial activity test according to the material and method of embodiment 1, wherein the Streptococcus mutans bacterial suspension concentration is 10 7 CFU / mL; after adding the sample, place it in an anaerobic incubator, and incubate anaerobically at 36°C for 28 h; the concentration of MTT test solution is 5.0 mg / mL, and incubate at an anaerobic constant temperature at 36°C for 1.5 h.

[0062] The results showed that compared with the control tobacco liquid YY0, the tobacco liquid YY2 added with tea extract had a significant inhibitory effect on Streptococcus mutans, and the antibacterial rate of the original liquid was 35.32%, which was significantly higher than the 14.43% of the control liquid tobacco liquid. ...

Embodiment 3

[0064] Weigh 10.0g of the basic e-cigarette liquid sample, add clove extract according to the sensory evaluation results, the addition amount is 0.2g, the sample number is YY3; the control group is the basic e-cigarette liquid, the number is YY0.

[0065] Carry out antibacterial activity test according to the material and method of embodiment 1, wherein the Streptococcus mutans bacterial suspension concentration is 10 8 CFU / mL; after adding the sample, place it in an anaerobic incubator, and incubate anaerobically at 36°C for 30 h; the concentration of MTT test solution is 3.0 mg / mL, and incubate at an anaerobic constant temperature at 36°C for 1.5 h.

[0066] The results showed that compared with the control smoke liquid YY0, the smoke liquid YY3 added with clove extract had an inhibitory effect on Streptococcus mutans, and the bacteriostatic rates of the stock liquid and 1 / 2 stock liquid were 14.77% and 5.35%, respectively, which were comparable to those of the control smoke ...

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Abstract

Belonging to the technical field of electronic cigarette research and development, the invention relates to a method for rapid screening of electronic cigarette liquid based on streptococcus mutans. The method combines in-vitro bacteriostatic detection technique with cigarette liquid blending technology, and includes: firstly preparing cigarette liquids containing different plant extracts, then adopting microdilution bacteriostatic detection technique to screen the prepared cigarette liquids, thus obtaining the cigarette liquid with bacteriostatic activity. The method provided by the inventionhas the characteristics of: use of streptococcus mutans as the test strain, determination of the microdilution bacteriostatic detection system suitable for electronic cigarette liquid, high screeningdetection efficiency, and acquisition of a cigarette liquid formula with bacteriostatic activity within a short time, and provides effective technical support for acquisition of safe and high qualityelectronic cigarette liquid.

Description

technical field [0001] The invention relates to a method for rapidly screening electronic cigarette liquid based on Streptococcus mutans, and belongs to the technical field of research and development of electronic cigarettes. Background technique [0002] Electronic cigarettes, also known as electric atomized cigarettes, are a new type of tobacco product that is close to traditional cigarettes in terms of appearance, physiological feelings, psychological perception, and smoking methods. E-cigarettes transmit nicotine to the respiratory system through electronic heating means, and are mainly composed of tobacco rods, atomizers, suction nozzles, chargers and cartridges. E-liquid is the main smoking material of electronic cigarette products. It volatilizes and produces smoke under low temperature heating. Its formula is also one of the core technologies of electronic cigarettes. However, the aroma and smell of e-liquid smoke is quite different from that of traditional cigaret...

Claims

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Application Information

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IPC IPC(8): C12Q1/18
CPCC12Q1/18G01N2333/315
Inventor 米其利高茜段沅杏赵伟巩效伟楼牧梦刘晓艳郭绍翠夭建华
Owner CHINA TOBACCO YUNNAN IND
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