Tissue culture seedling method for top shoots of passiflora caerulea
A technology of Passiflora japonica and tip, which is applied in the field of group cultivation of shoots of Passiflora japonica, can solve the problems of easy browning, complicated operation procedures, unstable thermal detoxification effect of a single shoot tip, and the like, To achieve the effect of improving anti-oxidation and improving vitality
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Embodiment 1
[0018] Example 1: Trial in April 2016
[0019] (1) Disinfection of explants: Collect 50 spring shoots of passionflower purple fruit that have just germinated for 3 days, rinse them with tap water and distilled water 3 times, and cut the top shoots with a length of 3-4 cm to make explants; use 4500
[0020] mg.L -1 Vc+1000mg.L -1 Soak in citric acid distilled water for 45 minutes, rinse with distilled water 5 times. On the ultra-clean bench, use 2500mg.L -1 Soak in distilled potassium permanganate aqueous solution for 30 minutes, rinse with distilled water for 3 times; cut the explants into 2.0 cm long top tips, and then use 1000 mg.L -1 Mercury chloride aqueous solution disinfection 6min, distilled water rinse 5 times;
[0021] (2) Primary induction culture of axillary buds: inoculate the sterilized explants upright according to the growth polarity, medium formula: basic medium + 1.2g.L -1 AC+1.2 g.L -1 PVP +0.5mg.L -1 TDZ +0.05mg.L -1 NAA: A total of 50 plants were ...
Embodiment 2
[0027] Example 2 Test in April 2017
[0028] (1) Disinfection of explants: Collect 50 spring shoots of passionflower purple fruit that have just germinated for 5 days, rinse them with tap water and distilled water 3 times, and cut the top shoots with a length of 3-4cm to make explants; use 4500mg .L -1 Vc+1000mg.L -1 Soak in distilled citric acid aqueous solution for 45min, rinse with distilled water 5 times; use 2500mg.L -1 Soak in potassium permanganate distilled water for 30 minutes, rinse with distilled water 3 times; cut the explants into 2.0cm-long top tips, and then use 1000 mg.L -1 Mercury chloride aqueous solution disinfection 6min, distilled water rinse 5 times.
[0029] (2) Primary induction culture of axillary buds: inoculate the sterilized explants upright according to the growth polarity, medium formula: basic medium + 1.2g.L -1 AC+1.2 g.L -1 PVP +0.5mg.L -1 TDZ +0.05mg.L -1 NAA. A total of 50 plants were inoculated, and after 10 days of cultivation, 2 ...
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