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Tissue culture seedling method for top shoots of passiflora caerulea

A technology of Passiflora japonica and tip, which is applied in the field of group cultivation of shoots of Passiflora japonica, can solve the problems of easy browning, complicated operation procedures, unstable thermal detoxification effect of a single shoot tip, and the like, To achieve the effect of improving anti-oxidation and improving vitality

Active Publication Date: 2018-08-21
贵州省山地资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing detoxification methods for passionflower seedlings all have complex operating procedures, explants are all derived from mature organs or tissues that may carry viruses and bacteria, and the detoxification effect of single shoot tip heat treatment is unstable, etc.
The newly germinated top shoots of passionflower belong to young tissues with strong differentiation ability, almost no viruses and germs, and are easy to brown after disinfection treatment. At present, the young top shoots of passionflower are used as explants, and cell division is adopted. The combination of TDZ (thiadizuron) and auxin NAA to induce axillary bud differentiation, callus differentiation and clustered bud differentiation has not been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Trial in April 2016

[0019] (1) Disinfection of explants: Collect 50 spring shoots of passionflower purple fruit that have just germinated for 3 days, rinse them with tap water and distilled water 3 times, and cut the top shoots with a length of 3-4 cm to make explants; use 4500

[0020] mg.L -1 Vc+1000mg.L -1 Soak in citric acid distilled water for 45 minutes, rinse with distilled water 5 times. On the ultra-clean bench, use 2500mg.L -1 Soak in distilled potassium permanganate aqueous solution for 30 minutes, rinse with distilled water for 3 times; cut the explants into 2.0 cm long top tips, and then use 1000 mg.L -1 Mercury chloride aqueous solution disinfection 6min, distilled water rinse 5 times;

[0021] (2) Primary induction culture of axillary buds: inoculate the sterilized explants upright according to the growth polarity, medium formula: basic medium + 1.2g.L -1 AC+1.2 g.L -1 PVP +0.5mg.L -1 TDZ +0.05mg.L -1 NAA: A total of 50 plants were ...

Embodiment 2

[0027] Example 2 Test in April 2017

[0028] (1) Disinfection of explants: Collect 50 spring shoots of passionflower purple fruit that have just germinated for 5 days, rinse them with tap water and distilled water 3 times, and cut the top shoots with a length of 3-4cm to make explants; use 4500mg .L -1 Vc+1000mg.L -1 Soak in distilled citric acid aqueous solution for 45min, rinse with distilled water 5 times; use 2500mg.L -1 Soak in potassium permanganate distilled water for 30 minutes, rinse with distilled water 3 times; cut the explants into 2.0cm-long top tips, and then use 1000 mg.L -1 Mercury chloride aqueous solution disinfection 6min, distilled water rinse 5 times.

[0029] (2) Primary induction culture of axillary buds: inoculate the sterilized explants upright according to the growth polarity, medium formula: basic medium + 1.2g.L -1 AC+1.2 g.L -1 PVP +0.5mg.L -1 TDZ +0.05mg.L -1 NAA. A total of 50 plants were inoculated, and after 10 days of cultivation, 2 ...

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PUM

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Abstract

The invention provides a tissue culture seedling method for top shoots of passiflora caerulea. The method comprises technical links as follows: anti-browning and disinfection treatment of explants, initial induced culture of axillary buds, induced culture of callus, multiplication of subculture, induced culture of rooting and bottle seedling transplanting; tender top shoots are taken as the explants, soaked with an antioxidant Vc and citric acid mixed solution and subjected to soaking sterilization with a potassium permanganate solution, and anti-oxidative and anti-browning capacity and activity of the tender explants are improved; activated carbon and polyvinylpyrrolidone are added to culture media of the stages of initial induction of the axillary buds, induction of the callus and multiplication of subculture and used for adsorbing secreted harmful substances and inhibiting production of browning substances. With adoption of the method, contamination rate and browning rate of initialinduction of the top shoots of passiflora caerulea can be controlled to be 10% or lower; initial induction survival rate of the top shoots reaches 90% or higher, subculture multiplication coefficientof cluster buds is 5.0 or larger, and rooting rate is 90% or higher; survival rate of bottle seedling transplanting reaches 95.0% or higher. The method can meet the demand of industrialized seedlingproduction.

Description

technical field [0001] The invention relates to crop cultivation techniques, in particular to a method for group seedling cultivation of purple passion fruit top shoots. Background technique [0002] Passiflora (scientific name: Passiflora caerulea L.), a perennial evergreen climbing woody vine, is a fragrant and delicious fruit and has the reputation of "king of juice". Its fruit is rich in calcium, iron, phosphorus and other trace elements and 17 kinds of amino acids and other substances. In addition to eating, it also has good health care and medicinal effects. According to the determination, passion fruit fruit contains more than 135 kinds of aromatic substances, which are most suitable for processing into fruit juice. But the planting passion fruit disease is more serious, mainly contains root rot, phytophthora, anthracnose and mosaic disease etc., especially in the perennial orchard, the disease is more serious, causes dropped fruit and defective fruit to increase, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李安定张丽敏张建利席培宇蔡国俊彭熙龙秀琴王济红
Owner 贵州省山地资源研究所
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