Dracaena cochinchinensis callus seedling culture method

Abstract

The invention provides a Dracaena cochinchinensis callus seedling culture method. The Dracaena cochinchinensis callus seedling culture method comprises following steps: 1, explant selection; 2, induction of callus; 3, callus propagation culture; 4, callus differentiation culture, wherein 1 to 3mg / L thioridazine TDZ, 0.1 to 0.5mg / L of kinetin KT, 0.1 to 0.5mg / L of brassinolide, and 2mg / L of polyvinylpyrrolidone PVP are added into a MS differentiation culture medium; 5, rooting culture, wherein 0.5 to 1.0mg / L of naphthylacetic acid NAA and 2g / L of active carbon are added into a 1 / 2MS rooting culture medium; and 6, hardening seedling and transplanting. The Dracaena cochinchinensis multiple shoot propagation coefficient is increased to be 5 to 12 times of an original value, obtained multiple shoots are strong, rooting is easy to realize after inoculation to a rooting medium, rooting rate is 90% or higher, transplanting survival rate is 95% or higher, and a large amount of Dracaena cochinchinensis seedlings suitable for plantation can be cultured in a short time.

Description

technical field [0001] The invention relates to the technical field of seedling asexual propagation, in particular to a method for forming seedlings from callus of Dracaena glabrata. Background technique [0002] Dracaena cochinchinensis (Lour.) S.C.Chen belongs to the genus Dracaena of Liliaceae, also known as Millennium Tree, Dracaena cochinchinensis, and Dracaena cochinchinensis. Blood stasis, swelling and pain relief, astringent and hemostatic effects, mainly used for bruises, bleeding from wounds and abdominal cramps, etc. It is a national key protected wild plant, an endangered and rare species. Sword leaf dracaena has high medicinal value and is a traditional Chinese medicinal material in my country. . Dracaena resources in our country are getting less and less, and the consumption of traditional Chinese medicine resources has risen sharply. In recent years, the market demand for dracaena has been increasing, which has led to the devastating logging of wild resource...

AI Technical Summary

Problems solved by technology

For this reason, the difficulty of the tissue culture of Dracaena flavinica is: problem one, to explore the optimal hormone factor and condition of callus induction of Dracaena glabrata so as to increase the induction rate of callus; problem two, by adding Anti-browning substances, changing the culture environment, etc. to find better ways to inhibit browning, in order to effectively reduce the browning rate, and establish a high-quality Dracaena callus culture system; Question 3: Explore Dracaena Callus differentiation, optimal hormone factors and conditions for rooting culture to increase the proliferation coefficient and rooting rate of Dracaena glabrata clustered shoots

At present, there are only two comparative documents on the propagation of Dracaena glabrata using tissue culture technology. Comparative document 1: the patent application number is "CN201510755473.7", and the patent name is "A kind of Dracaena glabrata loose callus induction Method” is the solution to the above-mentioned technical problem 1; reference document 2: the patent application number is “CN201410735185.0”, and the patent name is “A method for inducing callus and preventing browning of Dracaena glabrata”, which solves all Described technical problem two; the above two comparative documents have not explored and solved the best hormone factors and conditions of the differentiation of the follow-up Dracaena callus, rooting culture in the third problem

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