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Tissue Culture Propagation Method of Dianthus brevis

A technology of Dianthus brevifolia and tissue culture, applied in horticultural methods, botany equipment and methods, plant cells, etc., can solve the problems of increased toxins, difficulty in multiplying seedlings, and affecting the robust growth of plants, etc., to meet production needs , The effect of solving the problem of large-scale seedling raising

Inactive Publication Date: 2015-09-02
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main breeding methods of Dianthus brevifolia are cutting and division breeding, but this breeding method is difficult to achieve a large number of seedlings in a short period of time, and after multiple generations of division, the toxins in the plant will increase, which will affect the robust growth of the plant; and The use of biotechnology tissue culture technology can accelerate its propagation speed and obtain a large number of test-tube seedlings, which can meet the needs of the seedling market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032](1) Extraction and disinfection of explants: cut short-petalled dianthus into 2-3cm bud stems as explants for disinfection; first, drop 2-3 drops of detergent into a beaker filled with 50ml tap water , put the explants into a beaker and stir gently for 2 minutes, then use cotton to clean the dirt on the surface of the explants, and rinse with tap water for 8-10 minutes; move the ultra-clean workbench, soak it in 75% ethanol for 30 seconds, and use sterile water (after Distilled water after autoclaving) rinse once, then sterilize with 50ml of 0.1% mercury liter added with 1-2 drops of Tween-20 for 6-8min, rinse with sterile water for 3-5 times, and finally place the sterilized Stainless steel dishes, cut into 1.0-1.5cm long bud stem segments, to obtain sterile explants.

[0033] (2) Acquisition of sterile test-tube plantlets: Place the sterilized explants in the induction medium for adventitious bud induction (temperature is 23-25°C, light intensity is 1500-2000lux, light...

Embodiment 2

[0044] Other is basically the same as embodiment 1, only the following content is different:

[0045] In step (3), the proliferation medium uses MS as the basic medium, and additionally 4.5g·L -1 Agar, 30g·L -1 Sucrose, 1g·L -1 Activated carbon, the medium pH value is 5.8, and add 1.0mg·L -1 6-benzyl adenine (6-BA), 0.5mg·L -1 Naphthaleneacetic acid (NAA), 0.3mg·L -1 indole acetic acid (IAA); the proliferation coefficient of adventitious buds is 6.1.

[0046] In step (4), the strong seedling medium uses MS as the basic medium, and adds 4.5g·L -1 Agar, 30g·L -1 Sucrose, 1g·L -1 Activated carbon, the medium pH value is 5.8, and add 0.8mg·L -1 6-benzyl adenine (6-BA), 1.0mg·L -1 naphthaleneacetic acid (NAA), the secondary multiplication coefficient of adventitious buds is 2.9;

[0047] In step (5), the rooting medium uses 1 / 2MS as the basic medium, and additionally 4.5g·L -1 Agar, 30g·L -1 Sucrose, 1g·L -1 Activated carbon, the medium pH value is 5.8, and add 0.1mg·L...

Embodiment 3

[0050] Other is basically the same as embodiment 1, only the following content is different:

[0051] In step (3), the proliferation medium uses MS as the basic medium, and additionally 4.5g·L -1 Agar, 30g·L -1 Sucrose, 1g·L -1 Activated carbon, the medium pH value is 5.8, and add 2.0mg·L -1 6-benzyl adenine (6-BA), 1.0mg·L -1 Naphthaleneacetic acid (NAA), 0.5mg·L -1 indole acetic acid (IAA); the proliferation coefficient of adventitious buds was 4.3.

[0052] In step (4), the strong seedling medium uses MS as the basic medium, and adds 4.5g·L -1 Agar, 30g·L -1 Sucrose, 1g·L -1 Activated carbon, the medium pH value is 5.8, and add 1.5mg·L -1 6-benzyl adenine (6-BA), 2.0mg·L -1 naphthaleneacetic acid (NAA), the secondary multiplication coefficient of adventitious buds is 3.2;

[0053] In step (5), the rooting medium uses 1 / 2MS as the basic medium, and additionally 4.5g·L -1 Agar, 30g·L -1 Sucrose, 1g·L -1 Activated carbon, the medium pH value is 5.8, and add 1.0mg·...

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Abstract

The invention discloses a tissue culture and propagation method for brachystemma calycinum, and develops a culture medium used together with the same. By using stems with buds as explants, the method has the advantages of large number of raw materials, and convenience in material accessing. By tissue culture propagation, the culture cycle can be shortened and high-quality seedlings of brachystemma calycinum can be rapidly propagated. The propagation coefficient of 30d buds cultured by the method can reach 6.1 times, the rooting rate of tissue culture seedlings can reach more than 92.6% through expanding propagation, strong seedlings and rooting culture, and the survival rate after transplanting matrix can reach more than 89.5%. Therefore,the method solves the scale seedling problem of brachystemma calycinum, is beneficial to mass production of seedlings, meets market demands, and has a high application value.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture and propagation, and in particular relates to a method for tissue culture and propagation of Dianthus brevifolia. Background technique [0002] Dianthus short petals, derived from the plant Brachystemma calycinum D.Don of Caryophyllaceae, is a single genus plant of Caryophyllaceae; its root or whole herb is used as medicine and is called Achyranthes bidentata (zhuang), Cramp grass (yao) etc., taste sweet, light, flat in nature; have heat-clearing and detoxifying, relaxing tendons and collaterals, expelling wind and dehumidification, diuresis and detumescence, the effects of osteosynthesis and granulation, used for rheumatic arthralgia, bruises, osteosynthesis, nephritis edema, osteomyelitis, Sore boils, lymph node tuberculosis and other diseases. Dianthus short petals are distributed in Guangxi, Sichuan, Yunnan and other provinces and regions in my country, and are mainly produced in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 闫志刚韦莹董青松吴庆华胡秀月黄保成黄英群刘凡
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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