A kind of method that Dracaena glabra callus becomes seedling

Abstract

The invention provides a method for seedling formation of Dracaena glabrata callus, which specifically comprises the following steps: (1) selection of explants; (2) induction of callus; (3) callus proliferation and cultivation (4) Callus differentiation culture, adding 1-3mg / L thiadizuron TDZ, 0.1-0.5mg / L kinetin KT, 0.1-0.5mg / L brassinolide and 2mg / L polyvinylpyrrolidone PVP; (5) Rooting culture, add 0.5-1.0mg / L NAA and 2g / L activated carbon to 1 / 2MS rooting medium; (6) Hardening and transplanting. The multiplication coefficient of clustered buds of Dracaena saponifera reaches 5-12 times. The clustered buds are strong and easy to take root after being inoculated into the rooting medium. The rooting rate is over 90%, and the transplanting survival rate is over 95%. It can be cultivated in a short time. A large number of dracaena seedlings suitable for cultivation have been produced.

Description

technical field [0001] The invention relates to the technical field of seedling asexual propagation, in particular to a method for forming seedlings from callus of Dracaena glabrata. Background technique [0002] Dracaena cochinchinensis (Lour.) S.C.Chen belongs to the genus Dracaena of Liliaceae, also known as Millennium Tree, Dracaena cochinchinensis, and Dracaena cochinchinensis. Blood stasis, swelling and pain relief, astringent and hemostatic effects, mainly used for bruises, bleeding from wounds and abdominal cramps, etc. It is a national key protected wild plant, an endangered and rare species. Sword leaf dracaena has high medicinal value and is a traditional Chinese medicinal material in my country. Dracaena resources in our country are getting less and less, and the consumption of traditional Chinese medicine resources has risen sharply. In recent years, the market demand for dracaena has been increasing, which has led to the devastating logging of wild resources o...

AI Technical Summary

Problems solved by technology

For this reason, the difficulty of the tissue culture of Dracaena flavinica is: problem one, to explore the optimal hormone factor and condition of callus induction of Dracaena glabrata so as to increase the induction rate of callus; problem two, by adding Anti-browning substances, changing the culture environment, etc. to find better ways to inhibit browning, in order to effectively reduce the browning rate, and establish a high-quality Dracaena callus culture system; Question 3: Explore Dracaena Callus differentiation, optimal hormone factors and conditions for rooting culture to increase the proliferation coefficient and rooting rate of Dracaena glabrata clustered shoots

At present, there are only two comparative documents on the propagation of Dracaena glabrata using tissue culture technology. Comparative document 1: the patent application number is "CN201510755473.7", and the patent name is "A kind of Dracaena glabrata loose callus induction Method” is the solution to the above-mentioned technical problem 1; reference document 2: the patent application number is “CN201410735185.0”, and the patent name is “A method for inducing callus and preventing browning of Dracaena glabrata”, which solves all Described technical problem two; the above two comparative documents have not explored and solved the best hormone factors and conditions of the differentiation of the follow-up Dracaena callus, rooting culture in the third problem

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