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Highly active tea pigment and preparation method thereof

A technology for highly active tea and tea pigment, which is applied in the field of highly active tea pigment and its preparation, can solve the problems of easy destruction of heat-sensitive components, large energy consumption for pretreatment of raw materials, long leaching time and the like, and achieves a simple and easy extraction method. , The effect of reducing protein content and shortening extraction time

Inactive Publication Date: 2018-08-17
金华市艾力生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional leaching method, there are disadvantages such as long leaching time, high labor intensity, high energy consumption for raw material pretreatment, and easy damage of heat-sensitive components.
The traditional extraction method of tea pigment in tea leaves is commonly used by boiling water decoction. This method has a high heating temperature, which will cause the polyphenols in the tea pigment to polymerize and deepen the color; while the organic solvent extraction method uses ethanol aqueous solution to extract the tea pigment, and the extraction rate is improved. The production cost is relatively high, and there are many impurities

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A preparation method of highly active tea pigment, comprising the following steps:

[0017] 1) Weigh the pulverized Longjing green tea, pass through a 100-mesh sieve after crushing, add distilled water according to the ratio of material to liquid 1g:12mL, put the mixture of material and liquid into the refrigerator at -5°C, freeze completely, and then use microwave to quickly freeze the frozen product Melt for use, the microwave power is 400W, and the extraction time is 8 minutes; 2) Place the extract in a high-speed desktop centrifuge, centrifuge at a rate of 3000r / min for 10 minutes, and take the supernatant to obtain a semi-finished solution containing tea pigment; Put the semi-finished product solution in a vacuum drying oven, dry and concentrate to 1 / 15 at 45°C, add 5 times the volume of 85% ethanol solution, stir evenly and let it stand for 30 minutes, adjust the pH to 3.2 with hydrochloric acid solution, and let it stand for 30 minutes. Then the solution was cent...

Embodiment 2

[0019] A preparation method of highly active tea pigment, comprising: freezing and thawing, extracting, and purifying, specifically comprising the following steps:

[0020] 1) Freezing and thawing: Weigh the pulverized Longjing green tea, pass through a 120-mesh sieve after crushing, add distilled water according to the ratio of material to liquid 1g:15mL, put the mixture of material and liquid into a refrigerator at -10°C, freeze completely, and then microwave The frozen matter is quickly thawed for use, the microwave power is 500W, and the extraction time is 9 minutes; freezing-thawing can quickly break the cell wall of the tea, and promote the rapid dissolution of the soluble tea pigment in the cell; microwave can be heated without temperature gradient, and the frozen matter can be quickly Melting, and a large number of microscopic cavities and defects are produced due to the rapid change of temperature and solid-liquid phase state, thereby effectively shortening the extract...

Embodiment 3

[0024] A kind of highly active tea pigment, the extraction rate of tea pigment in the final product is 32.5%, and the purity is 93.8%. Tea pigment can be used as a natural pigment in food processing and textile industries. Oxidation, enhancement of immune function, lowering blood lipids, two-way regulation of blood pressure and blood lipids, anti-atherosclerosis, lowering blood viscosity, improving microcirculation, inhibiting experimental tumors and other pharmacological effects, can be widely used in food, medical, health care and other fields.

[0025] A preparation method of highly active tea pigment, comprising: freezing and thawing, extracting, and purifying, specifically comprising the following steps:

[0026] Freezing and thawing: Weigh the pulverized Longjing green tea, pass through a 100-mesh sieve after crushing, add distilled water according to the ratio of material to liquid 1g:14mL, put the mixture of material and liquid into a refrigerator at -8°C, freeze comple...

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Abstract

The invention discloses a high-activity tea pigment and a preparation method thereof, the method comprises freeze-thawing, extraction and purification, the method is as follows: firstly crushing Longjing green tea, adding water to freeze, centrifuging after microwave thawing, taking supernatant, concentrating, adding an ethanol solution, acidifying, centrifuging, and taking supernatant, namely a tea pigment solution, adjusting the pH of the tea pigment solution to 7.0-7.5, filtering by suction, drying and extracting with chloroform to obtain the tea pigment. The beneficial effects are as follows: the possibility to separate the tea pigment out can be greatly reduced, the yield and content of the tea pigment can be increased, the protein content in the product can be reduced, and the purityof the product can be improved. The tea pigment can be used as a natural pigment used in the food processing and textile industry. At the same time, the tea pigment can be widely used in food, medical, health care and other fields.

Description

technical field [0001] The invention relates to the field of tea deep processing, in particular to a highly active tea pigment and a preparation method thereof. technical background [0002] Thousands of years ago, tea came to the world from China, and it is one of the three major beverages in the world together with coffee and cocoa. Tea pigment is a kind of natural pigment in tea with multiple functional activities. As a natural pigment, tea pigment has anti-oxidation, anti-aging, lowering blood pressure, cardiovascular protection, anti-allergic, anti-bacterial, anti-cancer and even anti-AIDS effects, and is widely used in food processing and pharmaceutical fields. Before 1990, only 0.6-1.6kg of tea pigment could be extracted per ton of tea, known as "green gold in medicine". Now with the continuous development of purification technology, it can be mass-produced. In the traditional leaching method, there are disadvantages such as long leaching time, high labor intensity...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C09B67/54C09B67/14
CPCC09B61/00C09B67/0017C09B67/0096
Inventor 周艳初
Owner 金华市艾力生物科技有限公司
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