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Use of Huangqin Decoction and Its Equivalent Components for Enhancing Sensitivity of Colon Cancer to Irinotecan Chemotherapy

A technology of irinotecan and Huangqin decoction, applied in the field of medicine, can solve the problems of not revealing Huangqin decoction, enhance the anti-colon cancer activity of irinotecan, and achieve the effects of enhancing sensitivity, enhancing chemotherapy sensitivity, and controlling quality

Active Publication Date: 2020-10-30
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no research has confirmed whether Huangqin Decoction can enhance the anti-colon cancer activity of irinotecan, nor has it revealed the combination of active ingredients in Huangqin Decoction that can play a synergistic role

Method used

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  • Use of Huangqin Decoction and Its Equivalent Components for Enhancing Sensitivity of Colon Cancer to Irinotecan Chemotherapy
  • Use of Huangqin Decoction and Its Equivalent Components for Enhancing Sensitivity of Colon Cancer to Irinotecan Chemotherapy
  • Use of Huangqin Decoction and Its Equivalent Components for Enhancing Sensitivity of Colon Cancer to Irinotecan Chemotherapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Huangqin Decoction enhances the chemosensitivity of colon cancer to irinotecan

[0025] 1. Test materials

[0026] 1. Instruments and reagents

[0027] RIPM1640 (Gibco-Thermo Fisher Scientific, USA); 0.25% trypsin, fetal bovine serum, penicillin-streptomycin solution (Biological Industries, Israel); DMSO (Sigma, USA); 6-well plate, 96-well plate (Corning company); multi-functional microplate reader (Infinite M200PRO); biological safety cabinet, CO 2 Cell culture incubator (Thermo Fisher Scientific, USA)

[0028] 2. Cell lines

[0029] Human colon cancer cell line HCT-116 was cultured in RIPM1640 complete medium containing 10% fetal bovine serum.

[0030] 3. Sample preparation of Huangqin Decoction

[0031] Preparation of Huangqin Decoction: Scutellaria baicalensis, peony, licorice, jujube (9g: 6g: 6g: 6g), soak in 400mL double distilled water for 30 minutes, adjust the plate heater to display a temperature of 330 ° C, heat for 20 minutes (boiling after a...

Embodiment 2

[0052] Embodiment 2: the mensuration of equivalent component group content in Huangqin Decoction (standard product external standard method)

[0053] The treatment process of the Huangqin Decoction sample is the same as in Example 1.

[0054] High performance liquid phase system Agilent 1100HPLC system (Agilent Technologies, USA)

[0055] Chromatographic conditions

[0056] The chromatographic column is Agilent Zorbax SB-C18 column (250mm×4.6mm ID, 5μm)

[0057] Detection wavelength: gallic acid, baicalein, scutellaria baicalensis, scutellarin A280nm; ammonium glycyrrhizinate 254nm; scutellarin 335nm.

[0058] Chromatographic conditions: mobile phase methanol (A)-0.1% phosphoric acid aqueous solution (B)

[0059] Elution gradient: 0-8min, 5%A→25%A; 8-10min, 25%A→38%A; 10-20min, 38%A→55%A; 20-35min, 55%A→100% A;

[0060] Column temperature: 43°C, flow rate: 1.0 mL / min.

[0061] According to the above-mentioned chromatographic conditions, the content of 6 active substances...

Embodiment 3

[0062] Example 3: Huangqin Decoction Equivalent Component Group Enhances Chemosensitivity of Colon Cancer to Irinotecan

[0063] In this experiment, each chemical component monomer (gallic acid, scutellarin, baicalein, wogonin, glycyrrhizinic acid, and scutellarin A) was weighed and dissolved in dimethyl sulfoxide. The content ratio in Huangqin Decoction (HQD) is mixed. After conversion, the final concentration of the active substance group (HB) is equivalent to the final concentration of Huangqin Decoction of 1000 μg / mL (MTT experiment) and 1000 μg / mL (flow cytometry apoptosis experiment). Test method is the same as embodiment 1.

[0064] 1. MTT assay to evaluate the effect of HB and HQ combined with irinotecan on cell viability

[0065] The results are shown in Table 4 and 5. Among them, * represents the comparison between the irinotecan single-use group and the combined use group, P<0.05 is represented by *, and P<0.005 is represented by **.

[0066] Table 4 HQD enhance...

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Abstract

The invention discloses a radix scutellariae soup, and application of an equivalent component group of the radix scutellariae soup to improvement of the chemosensitivity of the colon cancer on the irinotecan. The invention finds that the radix scutellariae soup can be beneficial to remarkably improving the chemosensitivity of the colon cancer on the irinotecan, namely, improving the killability ofthe irinotecan on the the colon cancer, the inhibiting effect on colon cancer cell proliferation, and the acceleration on colon cancer cell apoptosis, so that without remarkably reducing a chemotherapeutic effect of the irinotecan, the administration dosage of the irinotecan is reduced favorably, and the chemotherapy side effects of the irinotecan is relieved. The invention finds that through a composition prepared from gallic acid, scutellarin, baicalein, wogonin, glycyrrhizic acid and oroxylin A in the radix scutellariae soup, the chemosensitivity of the colon cancer on the irinotecan can be remarkably improved, and the medicine effect is basically consistent to the equivalent dosage of radix scutellariae soup; the find is helpful to develop a composition medicine with definite development components and controllable quality so as to replace the radix scutellariae soup for assisting colon cancer chemotherapy, and also helpful to adopt the composition as a quality control index to control the quality of the radix scutellariae soup.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the secondary development of classic famous prescriptions of traditional Chinese medicines, in particular to the use of Huangqin Decoction and its equivalent components for enhancing the chemotherapy sensitivity of colon cancer to irinotecan. Background technique [0002] Colon cancer is a common malignant tumor. With the improvement of people's living standards and changes in diet structure and other aspects, the incidence of colorectal cancer in my country is increasing year by year. According to statistics, there are 331,000 new cases of colorectal cancer in my country every year, and 159,000 patients die from the disease every year. More than 106,000 people in the United States are diagnosed with colorectal cancer each year. Chemotherapy is currently the mainstay of colorectal cancer treatment. [0003] Irinotecan (CPT-11) is a DNA topoisomerase I inhibitor, which is the first-line dr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/725A61P35/00A61K31/192A61K31/7048A61K31/352A61K31/704
CPCA61K31/192A61K31/352A61K31/704A61K31/7048A61K36/484A61K36/539A61K36/65A61K36/71A61K36/725A61P35/00A61K2300/00
Inventor 许风国汪偶戴小敏吕波张尊建
Owner CHINA PHARM UNIV
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