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Seedling raising device for preventing other bacterium infection and application thereof

A technology for raising seedlings and miscellaneous bacteria, which is applied in the field of seedling raising apparatuses for preventing miscellaneous fungus infection, can solve the problems of seedling decay, reduce mycorrhizal infection rate, insufficient illumination, etc., so as to reduce water loss, improve mycorrhizal infection rate, Increase the effect of photosynthesis

Pending Publication Date: 2018-07-31
LISHUI AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are problems in the cultivation of aseptic seedlings and mycorrhizal seedlings: (1) The cultivation of aseptic seedlings and mycorrhizal seedlings can only be cultivated in greenhouses or laboratories, which is difficult for large-scale production, and the production operability is not strong, and the investment in facilities is large , the cost of production increased substantially
(2) The large-scale production of sterile seedlings and mycorrhizal seedlings needs to be carried out in the nursery field under the conditions of greenhouse facilities. The nursery field and the surrounding environment contain indigenous mycorrhizal fungal propagules, such as spores and mycelium. During the cultivation process Very easy to be infected with miscellaneous bacteria, the success rate of aseptic and mycorrhizal seedling cultivation is not high
(3) At present, commonly used containers such as seedling pots, root control containers, and non-woven mesh bags are widely used in seedling cultivation. The common disadvantage of these containers is that there are holes in the bottom, and the roots of seedlings will grow out and extend into the nursery soil. Thereby greatly increasing the chance of indigenous mycorrhizal fungi infecting aseptic or mycorrhizal seedlings
Both root dipping and sowing inoculation methods require transplanting aseptic seedlings, which may easily cause damage to the root system of the seedlings during the transplanting slow seedling stage, and the seedlings will easily die when the weather is unfavorable, resulting in inoculation failure. Although the inoculation method has little effect on the seedlings, the chance of direct contact between the inoculant and the roots of the seedlings is small, and the inoculated bacteria grow very slowly and are not easy to survive for a long time, thereby reducing the mycorrhizal infection rate
(5) After the sterile seedlings are inoculated with the bacterial agent, especially in the three months immediately after the inoculation, the water management of the culture medium is very difficult and difficult to grasp accurately, which often leads to a decrease in the mycorrhizal infection rate and even the decay and death of the seedlings
(6) The currently used container for cultivating mycorrhizal seedlings is a general seedling container, which needs to be placed in a greenhouse or used under indoor conditions. It needs a fluorescent lamp to provide a light source, which consumes a lot of energy and costs high, which is not conducive to large-scale cultivation of mycorrhizal seedlings.
Insufficient light under greenhouse conditions affects the photosynthesis and mycorrhizal synthesis of seedlings
(6) When the seedlings inoculated with mycorrhizal fungi are directly placed in the open air using ordinary seedling containers, the seedling substrate is prone to dehydration, and the natural non-purpose mycorrhizal spores and other propagation will be transmitted to the substrate through rain, wind or underground soil , thus infecting non-target mycorrhizal fungi (such as puffballs, sclerotidia, etc.), which can easily cause pollution and cause the failure of the target mycorrhizal seedling cultivation

Method used

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  • Seedling raising device for preventing other bacterium infection and application thereof
  • Seedling raising device for preventing other bacterium infection and application thereof
  • Seedling raising device for preventing other bacterium infection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]A seedling raising device designed by the present invention to prevent infection by miscellaneous bacteria at least includes a seedling raising container 1 composed of a bottom surface and a side surface of the container. Different from the existing seedling-raising container, the side wall of the seedling-raising container 1 is provided with an air vent 1-1 and an inoculation hole 1-2, and the bottom surface of the seedling-raising container 1 is fixedly connected with a support ring 1-3, and the support ring is arranged in cooperation with The matrix bottom plate 1-5 is located at the bottom surface of the seedling cultivation container and the side wall of the seedling cultivation container of the matrix bottom plate 1-5 and is provided with drainage holes 1-4. In order to better prevent external bacteria from entering from the drainage hole 1-4, the supporting ring 1-3 designed by the present invention is composed of two rings, an outer ring 1-31 and an inner ring 1-3...

Embodiment 2

[0042] Adopt the device in the embodiment 1 to inoculate and cultivate the slash pine symbiosis seedling of Boletus spp., the method is as follows:

[0043] 1. Bacteria preparation:

[0044] 1) Strain tissue isolation: Collect the fruiting body of Boletus spp. in Lishui City in Chengxi Village, Liandu District, and bring it back to the laboratory for tissue isolation. Take the mung bean-sized tissue at the junction of the cap and the stipe, and place a special solid After culturing on the culture medium for one week, the mycelium was picked for purification and cultivation for later use. The formula of the special solid medium is: 100g of potatoes boiled and filtered to extract juice, 25g of glucose, 1.5g of potassium dihydrogen phosphate, 1g of magnesium sulfate, 1g of ammonium tartrate, 0.5ml of 1% iron citrate, 2.5ml of 0.1% zinc sulfate , vitamin B1 10mg, agar 15g, add water to 1000ml.

[0045] 2) Propagation of strains: Take the cultured strains and cut 1cm with a scalp...

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Abstract

The invention relates to a seedling raising device for preventing other bacterium infection. The device comprises a seedling raising container composed of a bottom surface and a side surface of a container. The device is characterized in that air holes and inoculation holes are formed in the side wall of the seedling raising container, a supporting ring is fixedly connected to the bottom surface of the seedling raising container, and a medium baseplate is cooperatively arranged on the supporting ring; drain holes are formed in the bottom surface of the seedling raising container and the side wall of the seedling raising container of the medium baseplate. The device can also be cooperatively provided with a light and rain shielding dustproof cover and a water guide device. The designed seedling raising device is simple in structure and convenient to use, mycorrhiza seedlings can be cultured under an open-air condition instead of a greenhouse facility or an indoor condition, the serial technical aim of large-scale, low-cost and high-quality culture of the mycorrhiza seedlings is achieved, the seedling raising device can also be used for nursery seedling raising at a drought region, the water can be greatly saved, and the survival rate of nursery stocks is increased.

Description

technical field [0001] The invention belongs to a seedling raising device, in particular to a seedling raising device for preventing the infection of miscellaneous bacteria. Background technique [0002] The cultivation of aseptic seedlings and mycorrhizal seedlings has high requirements on the environment. Once there are non-target strains in the environment, it is likely to infect the cultivated seedlings. It is carried out in the laboratory and cannot be cultivated in the open air because it is easy to be polluted. Among them, aseptic seedlings refer to seedlings whose roots are not infected with mycorrhizal fungi. The cultivation of aseptic seedlings must meet the following conditions: there are facilities for sheltering from wind and rain, and the seeds, seedling substrates, seedling containers, and irrigation water must be sterilized, and the cultivation process should avoid Infection by propagules of mycorrhizal fungi from wind, rain, and soil. Aseptic seedling cult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G9/029A01G18/64
CPCA01G9/021A01G9/0293A01G18/64
Inventor 宋艳冬薛振文郑晓鸣应国华吕明亮李伶俐高凤娟李泽建潘心禾
Owner LISHUI AGRI SCI
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