Seedling raising device for preventing other bacterium infection and application thereof
A technology for raising seedlings and miscellaneous bacteria, which is applied in the field of seedling raising apparatuses for preventing miscellaneous fungus infection, can solve the problems of seedling decay, reduce mycorrhizal infection rate, insufficient illumination, etc., so as to reduce water loss, improve mycorrhizal infection rate, Increase the effect of photosynthesis
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Embodiment 1
[0030]A seedling raising device designed by the present invention to prevent infection by miscellaneous bacteria at least includes a seedling raising container 1 composed of a bottom surface and a side surface of the container. Different from the existing seedling-raising container, the side wall of the seedling-raising container 1 is provided with an air vent 1-1 and an inoculation hole 1-2, and the bottom surface of the seedling-raising container 1 is fixedly connected with a support ring 1-3, and the support ring is arranged in cooperation with The matrix bottom plate 1-5 is located at the bottom surface of the seedling cultivation container and the side wall of the seedling cultivation container of the matrix bottom plate 1-5 and is provided with drainage holes 1-4. In order to better prevent external bacteria from entering from the drainage hole 1-4, the supporting ring 1-3 designed by the present invention is composed of two rings, an outer ring 1-31 and an inner ring 1-3...
Embodiment 2
[0042] Adopt the device in the embodiment 1 to inoculate and cultivate the slash pine symbiosis seedling of Boletus spp., the method is as follows:
[0043] 1. Bacteria preparation:
[0044] 1) Strain tissue isolation: Collect the fruiting body of Boletus spp. in Lishui City in Chengxi Village, Liandu District, and bring it back to the laboratory for tissue isolation. Take the mung bean-sized tissue at the junction of the cap and the stipe, and place a special solid After culturing on the culture medium for one week, the mycelium was picked for purification and cultivation for later use. The formula of the special solid medium is: 100g of potatoes boiled and filtered to extract juice, 25g of glucose, 1.5g of potassium dihydrogen phosphate, 1g of magnesium sulfate, 1g of ammonium tartrate, 0.5ml of 1% iron citrate, 2.5ml of 0.1% zinc sulfate , vitamin B1 10mg, agar 15g, add water to 1000ml.
[0045] 2) Propagation of strains: Take the cultured strains and cut 1cm with a scalp...
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