A graphene chip for specific capture of circulating tumor cells in whole blood and its preparation method and application

A graphene and graphene film technology, applied in the field of graphene chips and their preparation, can solve the problems of high detection cost and low detection efficiency, and achieve the effects of low cost, simple operation and enhanced capture efficiency

Active Publication Date: 2020-12-08
BEIJING SCITECH NANOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chip is expected to solve the current problems of low detection efficiency and high detection cost in early detection of cancer and postoperative monitoring.

Method used

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  • A graphene chip for specific capture of circulating tumor cells in whole blood and its preparation method and application
  • A graphene chip for specific capture of circulating tumor cells in whole blood and its preparation method and application
  • A graphene chip for specific capture of circulating tumor cells in whole blood and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0039] The sodium chloride prepared in this embodiment is the average size of the cubic graphene prepared by the template is 20 μm; taking circulating tumor cells EpCAM specific cells and EpCAM non-specific cells as circulating tumor cells to be captured as an example, the capture system of the present invention was used as an example. Further elaboration and validation. The method for specifically capturing circulating tumor cells by using the micro-nano structure on the surface of the prepared graphene chip and the specific recognition molecule to cooperate includes the following steps:

[0040] (1) Preparation of cubic graphene based on sodium chloride as template

[0041] (a) Preparation of cubic sodium chloride single crystal

[0042] Dissolve commercial sodium chloride powder in water to form saturated sodium chloride solution, control the temperature at 25 degrees, let stand, precipitate crystals, filter and dry to obtain cubic sodium chloride single crystal powder wit...

Embodiment 2

[0064] Potassium chloride prepared in this embodiment is the average size of the cube-structured graphene templated at 20 μm; taking circulating tumor cells EpCAM-specific cells and EpCAM non-specific cells as circulating tumor cells to be captured as an example, the capture system of the present invention For further elaboration and verification. The method for specific capture of circulating tumor cells using a graphene surface with a three-dimensional geometry comprises the following steps:

[0065] (1) Preparation of graphene chips based on potassium chloride as a template

[0066] (a) Preparation of cubic potassium chloride single crystal

[0067] Dissolve commercial potassium chloride powder in water to form a saturated solution of potassium chloride, control the temperature at 25 degrees, let stand, precipitate crystals, filter and dry to obtain potassium chloride single crystal powder with a cubic structure with an average side length of 20 μm.

[0068] (b) Preparation...

Embodiment 3

[0089] The average size of the three-dimensional geometric structure of graphene with copper chloride as a template prepared in this embodiment is 20 μm; taking circulating tumor cells EpCAM-specific cells and EpCAM non-specific cells as circulating tumor cells to be captured as an example, the capture system of the present invention For further elaboration and verification. The method for specific capture of circulating tumor cells using a graphene surface with a three-dimensional geometry comprises the following steps:

[0090] (1) Preparation of thin film chips composed of orthorhombic pyramidal graphene based on copper chloride as template

[0091](a) Preparation of orthorhombic pyramid copper chloride single crystal

[0092] Dissolve commercial cupric chloride powder in water to form a cupric chloride saturated solution, control the temperature at 25 degrees, let it stand still, precipitate crystals, filter and dry to obtain cupric chloride single crystal powder with an ...

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Abstract

The present invention provides a graphene chip for specifically capturing circulating tumor cells in whole blood and a manufacturing method and application thereof. The manufacture of the graphene chip comprises: using a single crystal structure having a three-dimensional geometric shape obtained by recrystallization of inorganic compound powder as a template; introducing a carbon source and causing the same to undergo high temperature pyrolysis to obtain a solution of graphene particles having a three-dimensional structure; performing suction filtration or spin coating of the solution to obtain a graphene substrate having a three-dimensional geometric micro-nano structure on a surface thereof; and modifying the graphene substrate with a specific recognition antibody to obtain the graphene chip that specifically captures circulating tumor cells. The antibody-modified, three-dimensional geometric micro-nano structured graphene chip can significantly improve the efficiency of capturing circulating tumor cells.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular, the invention relates to a graphene chip for specific capture of circulating tumor cells in whole blood, its preparation method and application. Background technique [0002] Circulating Tumor Cells (CTCs) refer to the tumor cells that come off from the primary tumor site and enter the blood circulation. Since circulating tumor cells appear in the early stages of cancer, it is of great significance to use circulating tumor cells as biomarkers to detect, monitor and analyze the number of circulating tumor cells in blood for early detection of cancer. At present, there are mainly the following methods for capturing circulating tumor cells, namely, immunomagnetic separation technology based on specific antibody-modified magnetic beads; microfluidic technology based on increasing the contact frequency between cells and the substrate; microfiltration technology based on cel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12N5/09C01B32/186
CPCC12M45/00C12N5/0694C12N2509/00C12M1/00C01B32/186G01N33/574
Inventor 樊俊兵王树涛江雷李冠男
Owner BEIJING SCITECH NANOTECH CO LTD
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