SNP molecular marker related to sheep kidding number properties, and applications thereof

Abstract

The invention provides a SNP molecular marker related to sheep kidding number properties, and applications thereof. The SNP molecular marker related to sheep kidding number properties is the 4389th base, which is A or G, of GLIS1 gene sequence represented by SEQ ID NO:1 of sheep. The invention also discloses a method used for detecting sheep GLIS1 gene type and predicting sheep average kidding number based on Sequenom MassARRAY register SNP technology. According to the method, the nucleotide at the 27726261bp site of the No.1 chromosome of sheep is subjected to mononucleotide type detection, and it is determined whether the sheep GLIS1 gene is a homozygous gene or a heterozygous gene based on the mononucleotide type detection results. GC type homozygous individuals with high kidding numberproperties and AA type homozygous individuals with high kidding number are selected and reserved through selection on sheep GLIS1 gene, GA heterozygous individuals with low kidding number is weeded out, so that sheep breeding capacity is improved, and potential application value on sheep large scale molecular breeding is achieved.

Description

technical field [0001] The invention relates to a molecular marker detection technology, in particular to a SNP molecular marker related to the litter size trait of sheep and its application. Background technique [0002] The litter size trait is one of the most important economic traits of sheep. Because it is controlled by micro-effect polygenes and has low heritability, it is difficult to improve rapidly by conventional breeding methods. Molecular technology can effectively and rapidly improve its genetic progress, so the identification Major genes or molecular genetic markers related to litter size are the key to modern molecular breeding. [0003] GLIS1 is the GLIS Family Zinc Finger 1 (GLIS Family Zinc Finger 1, GLIS1) gene, which is a protein highly expressed in the first and second cell stages of mouse oocytes and fertilized eggs, and plays an important role in mouse embryonic development. effect. The GLIS1 gene plays an important role in the reprogramming process ...

AI Technical Summary

Problems solved by technology

[0005] However, there is no research on the regulation of GLIS1 gene in sheep reproduction, and its research will help to mine more analytical markers related to sheep litter size

Traditional genotype detection methods mostly use PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) and PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) detection methods. Difficult to achieve high-throughput automated assays

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