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An ionic reagent capable of treating myocardial infarction and its preparation method and application

A myocardial infarction and ion technology, applied in the field of biomedical materials, can solve the problems of lack of source of donor organs, transplantation of tissue engineering materials, limited supply of stem cells, etc., to promote signal exchange between cardiomyocytes, promote maintenance of activity, and inhibit cardiomyocytes Apoptotic effect

Active Publication Date: 2021-01-12
SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods can only alleviate the patient's symptoms to a certain extent, and heart transplantation is limited by the lack of donor organ sources
In recent years, the development of stem cell (Science 2014, 345, 1247391) and tissue engineering (Stem Cells Transl.Med. 2014, 3, 1090-1099) therapy has brought new hope for the treatment of myocardial infarction. However, due to the limited supply of stem cells , low differentiation efficiency and the difficulty of tissue engineering material transplantation, degradation and safety issues are still unresolved, therefore, exploring effective new methods to repair damaged cardiovascular and myocardial tissue to rebuild cardiac function has become an urgent problem to be solved, and has very important research significance

Method used

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  • An ionic reagent capable of treating myocardial infarction and its preparation method and application
  • An ionic reagent capable of treating myocardial infarction and its preparation method and application
  • An ionic reagent capable of treating myocardial infarction and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Effects of "Si ion therapy" on cardiomyocytes and cardiac function recovery after myocardial infarction:

[0071] (1) Preparation of Si ion reagent

[0072] Preparation of CaSiO according to ISO 10993-5 standard 3 Extraction solution. The mass volume ratio of powder and DMEM or PBS is 200mgmL -1 Add the powder to the extraction solvent, seal it and place it in a constant temperature shaker at 37°C for 24 hours, centrifuge at 4000rpm for 10 minutes, take the supernatant and filter it through a 0.22μm bacterial filter to obtain 200mg mL -1 CaSiO 3 Extract the stock solution. 200mg mL -1 CaSiO 3 Dilute the extract solution with DMEM or PBS to 1 / 2, 1 / 4, 1 / 8, 1 / 16, 1 / 32, 1 / 64, 1 / 128 and 1 / 256, put it in a 4°C refrigerator for use, and The ion concentration was measured by inductively coupled plasma optical emission spectrometer (ICP-OES) (see Table 1).

[0073] Table 1 Concentration of Si in gradient dilution Si extract solution (μg mL -1 )

[0074] .

[0075] (...

Embodiment 2

[0093] The effect of "Sr ion therapy" on cardiomyocytes:

[0094] (1) Preparation of Sr ion reagent

[0095] The SrO leaching solution was prepared according to the ISO 10993-5 standard. The mass volume ratio of powder and DMEM is 200mg mL -1 Add the powder to the extraction solvent, seal it and place it in a constant temperature shaker at 37°C for 24 hours, centrifuge at 4000rpm for 10 minutes, take the supernatant and filter it through a 0.22μm bacterial filter to obtain 200mg mL -1 SrO leaching stock solution. 200mg mL -1 The SrO extraction stock solution was diluted with DMEM to 1 / 2, 1 / 4, 1 / 8, 1 / 16, 1 / 32, 1 / 64, 1 / 128 and 1 / 256, placed in a 4°C refrigerator for use, and The ion concentration was measured by inductively coupled plasma optical emission spectrometer (ICP-OES) (see Table 2).

[0096] (2) Effect of Sr ion reagent on the activity of NRCMs (in vitro)

[0097] Normoxic state: at 1.5×10 4 NRCMs were inoculated into a 96-well cell culture plate at a density of...

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Abstract

The present invention provides an ion reagent capable of treating myocardial infarction, and a preparation method therefor and an application thereof. The ion reagent comprises: a liquid having biocompatibility and a bioactive ion dispersed in the liquid, the bioactive ion being selected from at least one of Si, Sr, Mg, Zn, Cu, Co, Mn and Ca ions.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to an ion reagent that can be used for treating myocardial infarction, a preparation method and application thereof, and can be applied to the repair and reconstruction of damaged myocardium and cardiovascular. Background technique [0002] Myocardial infarction has become one of the greatest threats to human health, with high morbidity and mortality (Am.J.Cardiol.2012, 109, 187). Ischemic myocardial infarction is mostly caused by thrombus in the coronary artery, causing acute occlusion of the lumen, reducing or interrupting the blood supply, leading to myocardial ischemic necrosis (Circulation 2000, 101, 2981). Therefore, dredging blood vessel blockage and promoting blood vessel regeneration to restore blood supply can effectively inhibit myocardial cell apoptosis and necrosis, which is very important for the treatment of myocardial infarction. The clinical treatmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/34A61K33/24A61K33/06A61K33/32A61K33/30A61K9/08A61P9/10A61P9/00
CPCA61K9/0019A61K9/08A61K33/06A61K33/24A61K33/30A61K33/32A61K33/34A61K33/00A61K33/14A61P9/00A61K2300/00
Inventor 常江易敏邢敏区彩文陈敏生吴成铁
Owner SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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