Preparation method and application of multifunctional super-sensitive Zn<2+> two-photon detection fluorescence molecular probe

Abstract

The invention relates to a preparation method and application of a multifunctional super-sensitive Zn<2+> two-photon detection fluorescence molecular probe. Firstly, 2,4-dihydroxy benzaldehyde, diethyl glutaconate and anhydrous piperidine react in ethanol to obtain a product 1; the product 1 and acetic anhydride react in anhydrous pyridine to obtain a product 2; the product 2, osmium tetroxide andsodium periodate react in tetrahydrofuran to obtain a product 3; the product 3 and anhydrous potassium carbonate react in methyl alcohol to obtain a product 4; finally, the product 4 id dissolved into the ethanol; 2-hydrazinopyridine and glacial acetic acid are added; the steps of backflow, recrystallization and the like are performed; the multifunctional super-sensitive Zn<2+> two-photon detection fluorescence molecular probe is obtained. The probe is applicable to the qualitative and quantitative analysis of Zn<2+> in a biological sample; the detection is sensitive, accurate and fast; the probe can be applied to the relevant fields of analytical chemistry, life organic analytical chemistry, disease advanced diagnosis, medical clinic detection and the like.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and relates to a multifunctional ultrasensitive Zn 2+ Preparation method and application of fluorescent molecular probe for two-photon detection. Background technique [0002] Zinc is one of the essential elements for living organisms. Its content in the human body is second only to iron, and it is the second most transition metal element. Zinc ions widely exist in the cells of the human body and play an important role in many physiological activities such as cell metabolism, gene expression, body immunity and nerve transmission. At present, the detection method of zinc ion is mainly based on fluorescent molecular probe detection method; although the reported methods have their own advantages, in general, their detection response ratio (10-60 times) needs to be improved, and the quantitative method and detection method still need to be improved. Some detection limitations imposed by the needl...

AI Technical Summary

Problems solved by technology

This will lead to problems such as low detection limit of existing detection methods, inaccurate quantitative results, single detection method, unfavorable for naked eye detection, and limitations of imaging research.

These issues are very restrictive for the detection of trace zinc ions in cells

Images