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Bacillus velezensis strain and fungicide and application thereof

A technology of Bacillus Velez and Bacillus, applied in the field of microorganisms, can solve the problems of chemical prevention and control of environmental pollution and unsafety

Active Publication Date: 2018-07-10
北京绿色农华作物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of Bacillus strain with strong and broad-spectrum antibacterial activity in order to solve the pollution environment and unsafe problem that the chemical control exists in the prior art, and the effect of this bacterial strain in the prevention and treatment of plant fungal diseases application

Method used

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  • Bacillus velezensis strain and fungicide and application thereof
  • Bacillus velezensis strain and fungicide and application thereof
  • Bacillus velezensis strain and fungicide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Isolation and Identification of Bacillus Veles strain Bv01

[0028] Bacillus Velez strain Bv01 was obtained from soil, and soil samples were collected from Dayin Village, Raoyang County, Hengshui City, Hebei Province.

[0029] (1) Separation: Take a Erlenmeyer flask, put 15 glass beads into it, add 90 ml of distilled water, and sterilize at 121°C for 30 min. Weigh 10 g of soil sample into the above-mentioned sterilized Erlenmeyer flask, put it into a shaker at 150 r / min and shake for 15 min to obtain a 10-fold diluted soil suspension. Take 4 sterilized test tubes and add 9 ml sterilized distilled water to each test tube. Take 1 ml of the 10-fold diluted soil suspension in the above-mentioned test tube filled with 9 ml of distilled water to obtain a dilution of 10 2 times the soil dilution solution, and so on, until it is diluted to 10 5 times the soil dilution. will be diluted 10 3 times, 10 4 times and 10 5 Put the diluted soil solution at 80°C in a wa...

Embodiment 2

[0032] Example 2 Determination of the inhibitory effect of Bacillus veleisi strain Bv01 on phytopathogenic fungi

[0033] Preparation of PDA medium: Weigh 200 g of potatoes, wash, peel and chop them, add 800 ml of distilled water to boil for half an hour, filter with gauze, then add 20 g of glucose and 20 g of agar, dilute to 1000 ml with distilled water, 121 °C for 30 minutes.

[0034] Strain activation: Inoculate Curvularia geniculateum, Pythium ultima, Sclerotinia sclerotium, Alternaria solani, Fusarium graminearum, and Fusarium oxysporum on PDA medium, and cultivate at 28°C until the colony diameter is about 6 cm spare.

[0035] Confrontation culture: use a puncher with a diameter of 5 mm to punch the bacteria cake at the edge of the above-mentioned activated colony, and inoculate it in the center of the PDA plate. Then, take a new PDA plate, spread 200 ul of the suspension of Bacillus veleisi Bv01 (1 billion / ml) evenly on it, let it dry, and use a puncher with a diamete...

Embodiment 3

[0039] Example 3 Determination of antibacterial activity of metabolites of Bacillus veleisi strain Bv01

[0040] Preparation of metabolites: Streak-inoculate the Bacillus veleisi strain Bv01 on the slant medium, culture at 28°C for 48 hours, take a ring with an inoculation loop and inoculate it in 50 ml liquid LB medium, at 28°C, 180 r / After 24 h of min culture, 15 ml was transferred to 150 ml LB medium, and cultured in a shaker at 28 °C and 180 r / min for 72 h to obtain the fermentation broth of Bacillus veleisi strain Bv01. After centrifugation at 6000 r / min for 10 min, and then filtered with a bacterial filter, the metabolites of Bacillus Velez strain Bv01 can be obtained.

[0041] Preparation of PDA plates containing metabolites: 20 ml of the above fermentation products were added to 80 ml of melted PDA medium to prepare PDA plates containing 20% ​​metabolites of Bacillus veleisi strain Bv01 for use. Take the PDA plate added with the same amount of LB liquid medium as the...

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Abstract

The invention discloses a bacillus velezensis strain and application thereof in prevention and control of plant fungus diseases. The strain is a bacillus velezensis strain Bv01 with the accession number of CGMCC No. 12744. The bacillus velezensis strain Bv01 has strong inhibiting effects on mycelial growth of curvularia geniculata, pythium ultimum, sclerotinia rolfsii sacc, alternaria solani, fusarium garaminearum schwabe and fusarium oxysporum, and mycelial growth inhibition ratios are separately 85.17%, 91.54%, 90.78%, 78.14%, 75.69% and 86.67%; and the fermentation product of the bacillus velezensis strain Bv01 has obvious inhibition effect on the curvularia geniculate, the sclerotinia rolfsii sacc, the alternaria solani, the fusarium garaminearum schwabe and the fusarium oxysporum, andthe inhibition rates are separately 96.47%, 100.00%, 83.14%, 77.25% and 87.84%. The fermentation liquor of the bacillus velezensis strain Bv01 can effectively prevent and treat pythium aphanidermatum, scelerotium rolfsii and wheat scab. A fungicide which takes the fermentation liquor of the bacillus velezensis strain Bv01 as an active ingredient has high application value on agricultural production.

Description

technical field [0001] The present invention relates to the technical field of microorganisms, in particular to a kind of Bacillus veleisi ( Bacillus Velezensis ) strains and their agents and applications. Background technique [0002] Phytopathogenic fungi are the main pathogens that cause plant diseases. At present, there are more than 8,000 species of plant pathogenic fungi known, and the types of plant diseases caused account for 80% of the total number of plant diseases, involving more than 30,000 plant species. Fungal diseases reduce the output of 5 major food crops including rice, wheat, corn, potato and soybean by 125 million tons every year worldwide, bringing economic losses of at least 60 billion US dollars to global agriculture and seriously threatening food production. [0003] At present, the most commonly used method of controlling plant fungal diseases is chemical control method. Chemical control plays an important role in agricultural production, and has...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCC12N1/20A01N63/10C12R2001/07C12N1/205
Inventor 罗丽莲张云华郭利辉林梅根张雄鹏
Owner 北京绿色农华作物科技有限公司
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