Method for improving mycorrhiza seedling breeding of Tricholoma matsutake and application of method

Abstract

The invention belongs to the technical field of edible fungi cultivation, and particularly relates to a method for improving mycorrhiza seedling breeding of Tricholoma matsutake and application of themethod. The method comprises the steps of: performing separating and screening and identifying to obtain a Tricholoma matsutake strain YN1 which forms a symbiotic relationship with the rhizosphere ofa pine and has a preservation number of CCTCC NO:M2017719 by using a temperature difference method; adopting a large plate with a diameter of 14.5 cm as a mycorrhization container, adopting pine seeds as a test material, inoculating aseptic-germination pine seedlings and the Tricholoma matsutake strain YN1 to the plate on which cellophane is laid, fixing the pine seedlings by using a sterile barbed wire for cocultivation, performing stationary cultivation in an illumination incubator for 2-3 months, and observing an obvious Tricholoma matsutake mycorrhizal structure under a stereo microscope;and taking the Tricholoma matsutake mycorrhizal structure one year after transplantation is conducted, extracting mycorrhizal genomic DNA, and performing ITS molecule identification to obtain gel images of single bands. After comparison with NCBI, it is determined that the single bands are Tricholoma matsutake, and the accuracy of mycorrhization identification is 100%.

Description

technical field [0001] The invention belongs to the technical field of cultivation and cultivation of edible fungi, and in particular relates to a method and an application for improving matsutake mycorrhized seedling cultivation. Background technique [0002] Matsutake (Tricholoma matsutake) is a symbiotic edible fungus with extremely considerable nutritional and economic value. Matsutake not only has a special fragrance and delicious taste, but also is rich in polysaccharides, sterols, proteins, trace elements, vitamins, and various essential amino acids and other nutrients, which can resist oxidation and aging, prevent cancer and tumors, improve human immunity, prevent and treat Cardiovascular disease; therefore, it has long been sought after by people, especially in the Japanese market, and has become one of the most valuable wild edible fungi in the world. my country's matsutake mainly grows in some forest areas in the northeast and southwest regions with high altitude...

AI Technical Summary

Problems solved by technology

In this case, in addition to the protection of wild matsutake resources, research on the artificial domestication and cultivation of matsutake has always been a very meaningful topic. Numerous researchers have made their own explorations on this issue, but the vast majority The strains isolated by most researchers and the mycorrhizal structure formed have not undergone strict molecular identification, and the method used for mycorrhizal synthesis is difficult to ideally synthesize matsutake mycorrhizal seedlings outside the original place of matsutake

[0004] According to the retrieval, the application number is CN201010166605.X (a method for matsutake artificial propagation promotion technology) discloses a kind of matsutake fruiting body and spores, mycorrhizae and hyphae around the bacteria pond as propagation materials. Exploration of woodland management antler promotion technology and bacteria pond artificial antler promotion. It is said that this invention can be used as a measure to protect and promote the growth of matsutake in the original place of Yunnan matsutake to a certain extent. The inoculation test species reported in the literature, the growth rate of the purely cultured matsutake strain, the molecular identification, the microscopic observation of the mycorrhizal structure formed, the photos and data of the observation of the Hastelloy network and the mantle structure are not available. It is clearly shown; in addition, using matsutake fruiting bodies and spores, mycorrhizae and mycelium around the fungus pond to multiply, although it may have a certain effect in the original place, it is difficult to ideally synthesize the fungus pond away from the matsutake's original place matsutake mycorrhizal seedlings

For example, the research of Yoshiaki, 1992, etc. showed that the germination rate of matsutake spores varies greatly due to time, place, individual and fruiting body maturity, and the normal germination rate is only 10-35%, and the monospore mycorrhized seedlings , the hyphae do not undergo mating, and eventually cannot form fruiting bodies

[0005] Similarly, the application number is CN201610532499X (a method for establishing a symbiotic relationship between ectomycorrhizal fungi and Pinus plants). The method for seedling infection to form mycorrhiza is to infect the mycorrhizal seedlings in the sterilized cultivation medium by using sterilized Pinus aseptic seeds and liquid matsutake strains. , the symbiotic relationship between matsutake and several species of Pinus, unfortunately it also has no molecular identification of the formed mycorrhizal structure, microscopic observations, photographs and data of the observation of Harvey's network and mantle structure are not clearly shown , so the true success of its synthesis of matsutake mycorrhizal seedlings is debatable, because there are a large number of spores of fungi (including some broad-spectrum symbiotic fungi) in the natural environment, and they can control the mycorrhizal process at any time during mycorrhizal synthesis. Interfering and forming other forms of mycorrhizal structures), but not necessarily forming the ideal matsutake mycorrhizal, so the results of the inoculation test obtained from this will be unstable due to changes in the environmental conditions of inoculation and cultivation

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