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Compound microbial agent for treating soil pollution and preparation method and application thereof

A composite microbial inoculant and microbacteria technology, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of poor treatment effect of nitrobenzene compounds, complicated preparation process, and easy loss of bacterial cells. , to achieve the effect of simple and easy preparation method and effective degradation effect

Active Publication Date: 2018-07-06
江苏世邦生物工程科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the complex microbial bacteria in the prior art have a poor treatment effect on nitrobenzene compounds, and the preparation process is complicated, and the bacteria are easy to lose, resulting in waste of resources

Method used

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  • Compound microbial agent for treating soil pollution and preparation method and application thereof
  • Compound microbial agent for treating soil pollution and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Raw material components: Pseudomonas japonicum ATCC 33616 culture, Comamonas testosteroni ATCC 700441 culture, Microbacterium ATCC 31001 culture and Acidovorax delbrueckii DSM 50403 culture in a ratio of 1:2:1.5: 3.

[0031] Preparation and application: (1) Mix culture of Pseudomonas japonicum culture, Comamonas testosteroni culture, Microbacteria culture and Acidovorax delbrueckii culture by weight parts by using mixed culture medium A to obtain mixed Culture A, mixed medium A contains the following component concentrations: tryptone 8g / L, soytone 10g / L, beef extract 2g / L, H 3 BO 3 2g / L, NaH 2 PO 4 ·H 2 O 2g / L, NaCl 5g / L, agar 10g / L and MnCl 2 .4H 2O 0.2 g / L, pH value is 6.5;

[0032] (2) add nitrobenzene compound again in the mixed culture A of step (1), obtain the mixed culture B after domestication, the step of domestication is to add nitrobenzene compound in the culture medium of mixed culture A, Subcultured at 2 days, 4 days and 7 days respectively, so th...

Embodiment 2

[0036] Raw material components: Pseudomonas japonicum ATCC 33660 culture, Comamonas testosteroni ATCC 39523 culture, Microbacterium ATCC 31001 culture and Acidovorax delbrueckii ATCC 49664 culture in a ratio of 1:2.5:2: 3.5.

[0037] Preparation and application: (1) Mix culture of Pseudomonas japonicum culture, Comamonas testosteroni culture, Microbacteria culture and Acidovorax delbrueckii culture by weight parts by using mixed culture medium A to obtain mixed Culture A, mixed medium A contains the following component concentrations: tryptone 10g / L, soytone 15g / L, beef extract 3g / L, H 3 BO 3 4g / L, NaH 2 PO 4 ·H 2 O 3g / L, NaCl 7g / L, agar 15g / L and MnCl 2 .4H 2 O 0.25 g / L, pH value is 6.7;

[0038] (2) add nitrobenzene compound again in the mixed culture A of step (1), obtain the mixed culture B after domestication, the step of domestication is to add nitrobenzene compound in the culture medium of mixed culture A, Subcultured at 3 days, 5 days and 8 days respectively, s...

Embodiment 3

[0042] Raw material components: Pseudomonas japonicum ATCC 33660 culture, Comamonas testosteroni ATCC 39523 culture, Microbacterium ATCC 31001 culture and Acidovorax delbrueckii ATCC 49664 culture in a ratio of 1:3:2.5: 4.

[0043] Preparation and application: (1) Mix culture of Pseudomonas japonicum culture, Comamonas testosteroni culture, Microbacteria culture and Acidovorax delbrueckii culture by weight parts by using mixed culture medium A to obtain mixed Culture A, mixed medium A contains the following component concentrations: tryptone 12g / L, soytone 20g / L, beef extract 5g / L, H 3 BO 3 5g / L, NaH 2 PO 4 ·H 2 O 4g / L, NaCl 8g / L, agar 20g / L and MnCl 2 .4H 2 O 0.3g / L, pH value is 6.8;

[0044] (2) add nitrobenzene compound again in the mixed culture A of step (1), obtain the mixed culture B after domestication, the step of domestication is to add nitrobenzene compound in the culture medium of mixed culture A, Subcultured at 3 days, 6 days and 9 days respectively, so t...

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Abstract

The invention discloses a compound microbial agent. The compound microbial agent comprises the following raw material components: a pseudomonas japonica culture, a comamonas testosteroni culture, a microbacillus culture and an acidovorax delafieldii culture; according to the invention, multiple bacterial cultures are subjected to mixed fermentation to prepare the compound microbial agent; and thecompound microbial agent has an effective degradation effect on organic residues such as nitrobenzene compounds formed by insecticides or pesticides in soil, and furthermore, special strains are subjected to mixed cultivation and multiple strains are subjected to mixed fermentation to provide a simple and easy preparation method.

Description

technical field [0001] The invention relates to a microbial bacterial agent and its preparation method and application, in particular to a composite microbial bacterial agent for controlling soil pollution, its preparation method and application. Background technique [0002] Nitrobenzene compounds are widely used in the production of pesticides, dyes, explosives, rubber and other chemical products. Nitrobenzene compounds are widely used and can enter the environment through waste water and waste gas, and can also be caused by accidents and storage during transportation and production. A large amount of nitrobenzene pollutants in the environment mainly include nitrobenzene, nitrochlorobenzene, nitrotoluene, nitrophenol, nitroaniline and nitrobenzoic acid, etc. Some nitrobenzene compounds have the characteristics of stable chemical properties, high toxicity and easy bioaccumulation, and have been included in the list of priority monitoring pollutants by the US Environmental Pro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/14C12R1/38C12R1/01
CPCC09K17/14C12N1/20
Inventor 谢悦波姚竣耀刘才群
Owner 江苏世邦生物工程科技有限公司
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