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Preparation method and application of graphene-based hemoglobin sensor electrode

A technology of hemoglobin and graphene, which is applied in the field of biosensors, can solve the problem of low occult blood and achieve the effects of easy adsorption, direct detection and quantitative determination

Active Publication Date: 2020-07-10
INST OF METAL RESEARCH - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the concentration of fecal occult blood is usually low, and it is difficult to observe with the naked eye, so a process of enrichment and direct electrochemical determination is required

Method used

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  • Preparation method and application of graphene-based hemoglobin sensor electrode
  • Preparation method and application of graphene-based hemoglobin sensor electrode
  • Preparation method and application of graphene-based hemoglobin sensor electrode

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preparation example Construction

[0034] In the specific implementation process, the preparation method of a third-generation graphene-based hemoglobin sensor proposed by the present invention is firstly to drop-coat the graphene oxide solution on the cleaned sensor electrode, and then electrochemically reduce the graphite oxide ene into graphene for direct electron transfer of hemoglobin and active adsorption and enrichment on the electrode surface. Then the chitosan solution is drop-coated on the surface of the electrode, and the hemoglobin sensor electrode modified with graphene is obtained after drying at room temperature. The role of chitosan is to prevent the graphene from falling into the electrolyte and improve the stability of the sensor. The specific steps of the method are as follows:

[0035] (1) Preparation of the third-generation hemoglobin sensor of graphene:

[0036] Pretreatment of the glassy carbon electrode: firstly, the glassy carbon electrode Use A1 with particle sizes of 1.0 μm, 0.3 μ...

Embodiment 1

[0043] Firstly, a PBS solution with a concentration of 1 mg / ml graphene oxide and a chitosan acetic acid solution with a concentration of 0.2 wt % were prepared respectively. Then modify the electrode 1 whose surface has been polished, first drop 1 μl of the above-mentioned graphene oxide solution on the electrode, and then add 5 μl of 0.2wt% chitosan solution dropwise after the electrode is dried at room temperature. After the electrode was dried, the electrode was inserted into a 0.1 mol / L phosphate buffer solution and soaked for 2 hours to remove the graphene oxide that was not firmly bound to the electrode, and finally a graphene oxide modified electrode was obtained. The above-mentioned modified electrode is used as a working electrode, and a three-electrode system is formed with a saturated calomel electrode (reference electrode) and a platinum sheet electrode (counter electrode), and a phosphate buffer solution of 0.1mol / L is used as an electrolyte, and a cyclic Electro...

Embodiment 2

[0045] The preparation method of the graphene-modified electrode is the same as in Example 1. In order to investigate the electrode reaction process of hemoglobin on the graphene-modified electrode, the modified electrode is used as the working electrode and the silver / silver chloride reference electrode, and the platinum plate electrode constitutes three electrodes. Electrode system, using 0.06M sulfuric acid containing 2μg / mL hemoglobin as the electrolyte, tested the cyclic voltammetry curve at different scanning speeds, the results are shown in Figure 2, it can be seen from the figure that the reduction peak current on the cyclic voltammetry curve and The voltage scan rate is proportional, indicating that the electrode process is controlled by the electron transfer process on the electrode surface, that is, the adsorption process is controlled.

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Abstract

The invention belongs to the field of biosensors and specifically relates to a preparation method and application of a graphene based hemoglobin sensor electrode. Graphene and chitosan are compoundedto serve as a modification layer of an electrode; excellent biocompatibility and conductivity of the graphene facilitate direct electron transfer between hemoglobin and an electrode; and due to the excellent biocompatibility of the graphene, the agglomerated hemoglobin molecules are spread on the surface of the graphene, and the heme at the electroactive part is exposed, to realize direct electrontransfer between the hemoglobin and the electrode as well as direct detection of hemoglobin. According to the hemoglobin biosensor prepared in the invention, the graphene material is favorable for adsorbing and spreading hemoglobin, and also can realize direct electron transfer with the electrode; the biosensor has the advantages of high sensitivity, high stability, wide linear range and relatively strong anti-jamming capability, and can be widely applied to fecal occult blood screening of suspected gastrointestinal cancer patients.

Description

technical field [0001] The invention belongs to the field of biosensors, and in particular relates to a preparation method and application of graphene-based hemoglobin biosensor electrodes. Background technique [0002] Colorectal cancer is a common malignant tumor of the gastrointestinal tract, accounting for the first place in all gastrointestinal cancers, and its incidence is increasing year by year. According to the National Cancer Registry, from 1991 to 2005, the incidence of colorectal cancer was one of the three fastest-rising tumors. In Western society, the incidence rate is higher. According to statistics in recent years, there is one case of colorectal cancer in every 20 adults. Foreign data show that carrying out colorectal cancer screening can detect precancerous diseases, and through appropriate intervention, the incidence and mortality of tumors can be effectively reduced. Although the diagnostic technology is constantly developing, many patients are diagnose...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/327
Inventor 英哲张睿杨卫东郝亚斌孙宝明宋纯李记彬曾尤成会明
Owner INST OF METAL RESEARCH - CHINESE ACAD OF SCI
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