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Application of male hippocampus japonicus to preparation of medicine or preparation for improving desperation and helplessness behaviors of depression

A depression and drug technology, applied in the field of medicine, can solve problems such as unclear molecular mechanism, achieve the effect of improving depression and desperate behavior, and restoring the level of neurotransmitters in the brain

Inactive Publication Date: 2018-06-29
GUANGDONG OCEAN UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no relevant reports on whether the hippocampus can improve the behavioral manifestations of depression, helplessness, or even suicide, and the molecular mechanism of its action is even less clear.

Method used

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  • Application of male hippocampus japonicus to preparation of medicine or preparation for improving desperation and helplessness behaviors of depression
  • Application of male hippocampus japonicus to preparation of medicine or preparation for improving desperation and helplessness behaviors of depression
  • Application of male hippocampus japonicus to preparation of medicine or preparation for improving desperation and helplessness behaviors of depression

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Effect of Male Hippocampus on Forced Swimming Test in Mice

[0034] 1. Establishment of mouse depression model

[0035] Refer to Papp and Willner (Papp et al. , 1992) and modified for applicability, the animals were repurchased and adapted to the laboratory for one week, and randomly numbered and divided into groups, a total of 4 groups were set up, as shown in Table 1:

[0036] Table 1 Grouping of experimental animals

[0037]

[0038] The CUMS group and the CUMS+Seahorses group were exposed to the following stressors as model treatments; the Control group and Seahorses groups were reared under standard conditions without stress treatment.

[0039] All the stressors were randomly operated independently, and it was guaranteed not to be repeated within a week to prevent the animals from adapting. Two stressors were operated independently every day, and after 8 weeks of continuous stress, the behavior was measured. Stress sources include: water deprivatio...

Embodiment 2

[0044] Example 2 Effect of Male Hippocampus on the Contents of Neurotransmitters and Metabolites in Mouse Hippocampus

[0045] 1. Extraction of fatty acids from mouse hippocampal brain tissue

[0046] After the mice were decapitated and sacrificed, the hippocampal tissues were quickly isolated on ice, frozen in liquid nitrogen, and then transferred to -80°C for storage.

[0047]Total fatty acid extraction: tissue homogenate, operate in a fume hood, add 10.5mL chloroform / methanol (the volume ratio of the two is 2:1) solution to each tube, 100μL content is 2mg / mL C23:0 as internal standard, fill into N 2 Close the lid tightly, vortex for 30s, and then ultrasonically clean for 10-15min until there is no obvious block; vortex again for 30s, then add 2.6mL 0.88% NaCl solution, fill with N 2 Then close the lid tightly, vortex again for 15s; centrifuge at 500r / min for 20min.

[0048] Preparation of filter unit: with CH 2 CL 2 Drop washing glass funnel (about 6-10cm in diameter),...

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Abstract

The invention belongs to the technical field of medicines and in particular discloses application of male hippocampus japonicus to preparation of a medicine or a preparation for improving desperationand helplessness behaviors of depression. According to the application provided by the invention, a mouse depression model is established through chronic mild unpredictable stimulation and feed containing the male hippocampus japonicus is fed to a mouse; the depression behavior resisting effect of the male hippocampus japonicus is evaluated through a forced swimming test; a possible mechanism is detected through molecular biological techniques including an HPLC (High Performance Liquid Chromatography) and the like. The research finds out that changes of hippocampus brain area neurotransmittersand metabolites thereof of the mouse are regulated and controlled by the male hippocampus japonicus and an intracerebral neurotransmitter level of the mouse is recovered, so that the desperation andhelplessness behaviors induced by CUMS (Chronic Unpredictable Stress) are improved and a novel way is provided for researching and developing a new anti-depression medicine.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of the male hippocampus in the preparation of drugs or preparations for improving the helpless behavior of depression. Background technique [0002] Hippocampus is a traditional and precious marine Chinese herbal medicine in my country, which has the functions of warming kidney and strengthening yang, and dissolving swelling. It is commonly used for impotence, enuresis, dyspnea due to kidney deficiency, accumulation of lumps in the abdomen, traumatic injuries; external treatment of carbuncle and furuncle. Research on its chemical composition shows that hippocampus has high nutritional and medicinal value and a variety of chemically active components: the protein content is as high as 70%, as well as fatty acids and lipid components, steroidal compounds, phospholipid components and more. inorganic elements, etc. Studies have shown that the hippocampus also has a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/60A61P25/24
CPCA61K35/60
Inventor 张才李康伟宋采张永平张翼千忠吉赵云涛刘宇雄崔钰华
Owner GUANGDONG OCEAN UNIVERSITY
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