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Method for synchronously extracting, separating and measuring solanine and solanum nigtum polysaccharide in solanum nigrum green fruit

A technology of simultaneous extraction and midsolanine, applied in material separation, measuring device, analyzing materials, etc., can solve the problems of failing to achieve simultaneous extraction and separation of two active components, single extraction of solanine, etc., and achieve simultaneous extraction and separation, simple method, good reproducibility and stability

Inactive Publication Date: 2018-06-26
JILIN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction of effective components of Solanum nigrum is a single extraction of solanine or polysaccharides of Solanum nigrum, and the simultaneous extraction and separation of the two effective components cannot be realized.

Method used

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  • Method for synchronously extracting, separating and measuring solanine and solanum nigtum polysaccharide in solanum nigrum green fruit
  • Method for synchronously extracting, separating and measuring solanine and solanum nigtum polysaccharide in solanum nigrum green fruit
  • Method for synchronously extracting, separating and measuring solanine and solanum nigtum polysaccharide in solanum nigrum green fruit

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Instruments and medicines

[0026] FA104N 1 / 10,000 analytical balance (manufactured by Shanghai Precision Scientific Instrument Co., Ltd. balance instrument factory); Japan Shimadzu ultraviolet spectrophotometer UV2500; American Agilent 1100 high performance liquid chromatography; HN99-IID ultrasonic cell wall breaker (Shanghai Hannuo Instrument Co., Ltd.).

[0027] Australian solanine and Australian solanine are all provided by Zhengzhou Alpha Chemical Co., Ltd., and the purity is higher than 99.9%; absolute ethanol (chromatographically pure), acetonitrile (chromatographically pure), absolute ethanol (analytical pure) and potassium carbonate (analytical pure ) were purchased from Sinopharm Chemical Reagent Co., Ltd.

[0028] 2. The specific steps are as follows:

[0029] (1) Preparation of solanine reference substance solution: Accurately weigh solanine and solanine reference substances, place them in 5mL volumetric flasks respectively, add 3mL absolute ethanol (c...

Embodiment 2

[0038] 1. Instruments and medicines

[0039] FA104N 1 / 10,000 analytical balance (manufactured by Shanghai Precision Scientific Instrument Co., Ltd. balance instrument factory); Japan Shimadzu ultraviolet spectrophotometer UV2500; American Agilent 1100 high performance liquid chromatography; HN99-IID ultrasonic cell wall breaker (Shanghai Hannuo Instrument Co., Ltd.).

[0040] Australian solanine and Australian solanine are all provided by Zhengzhou Alpha Chemical Co., Ltd., and the purity is higher than 99.9%; absolute ethanol (chromatographically pure), acetonitrile (chromatographically pure), absolute ethanol (analytical pure) and potassium carbonate (analytical pure ) were purchased from Sinopharm Chemical Reagent Co., Ltd.

[0041] 2. The specific steps are as follows:

[0042] (1) Preparation of solanine reference substance solution: Accurately weigh solanine and solanine reference substances, place them in 5mL volumetric flasks respectively, add 3mL absolute ethanol (c...

Embodiment 3

[0053] 1. Instruments and medicines

[0054] FA104N 1 / 10,000 analytical balance (manufactured by Shanghai Precision Scientific Instrument Co., Ltd. balance instrument factory); Japan Shimadzu ultraviolet spectrophotometer UV2500; American Agilent 1100 high performance liquid chromatography; HN99-IID ultrasonic cell wall breaker (Shanghai Hannuo Instrument Co., Ltd.).

[0055] Australian solanine and Australian solanine are all provided by Zhengzhou Alpha Chemical Co., Ltd., and the purity is higher than 99.9%; absolute ethanol (chromatographically pure), acetonitrile (chromatographically pure), absolute ethanol (analytical pure) and potassium carbonate (analytical pure ) were purchased from Sinopharm Chemical Reagent Co., Ltd.

[0056] 2. The specific steps are as follows:

[0057] (1) Preparation of solanine reference substance solution: Accurately weigh solanine and solanine reference substances, place them in 5mL volumetric flasks respectively, add 3mL absolute ethanol (c...

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Abstract

The invention discloses a method for synchronously extracting, separating and measuring solanine and solanum nigtum polysaccharide in solanum nigrum green fruit, the method comprises the steps that ultrasonic wall-breaking, two aqueous phase extraction and high performance liquid phase are combined, synchronous extraction of the solanine and the solanum nigtum polysaccharide in the solanum nigrumgreen fruit is realized through ultrasonic wall-breaking, separation of the solanine and the solanum nigtum polysaccharide is completed through two aqueous phase extraction, and the contents of the solanine and the solanum nigtum polysaccharide are detected with the high performance liquid phase and a phenol-sulfuric acid method. The method is simple, easy to operate and good in reproducibility and can be used for synchronous extraction and separation of the solanine and the solanum nigtum polysaccharide in the solanum nigrum green fruit, and a new technological means is provided for extracting and separating the solanum nigtum effective ingredients in the whole solanum nigtum herb.

Description

technical field [0001] The invention relates to the field of extracting and separating effective components of nightshade, in particular to a method for synchronously extracting, separating and measuring solanine and solanum polysaccharide in green nightshade fruit. Background technique [0002] Solanum nigrum belongs to the Solanaceae plant. It is a traditional Chinese herbal medicine. It is cold in nature and bitter in taste. It has the functions of clearing away heat and detoxification, promoting blood circulation and reducing swelling. The whole plant of Solanum nigrum can be used medicinally, and can treat various diseases such as sores, tracheitis, cancer, cystitis, dysuria, dysentery, and sore throat. Solanum nigrum is a good medicinal plant, and its main active ingredients are solanine and solanum polysaccharide. . [0003] Solanine, as a glycoalkaloid, was first discovered by Desfosses in the early 19th century. Solanine is a total biological alkaloid extracted f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/74G01N30/06
CPCG01N30/02G01N30/06G01N30/74G01N2030/062G01N2030/065
Inventor 逯洋孙卓孙艳涛丛飚于萍于淼杨雪晴
Owner JILIN NORMAL UNIV
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