A kind of purification method of pleurotus eryngii polysaccharide
The technology of a polysaccharide and a purification method of Pleurotus eryngii is applied in the field of purification of Pleurotus eryngii polysaccharide, which can solve the problems of small volume and time-consuming processing of samples, and achieve the effects of improving the yield and purity of polysaccharide, wide application range and good effect.
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Embodiment 1
[0027] (1) Extraction of Pleurotus eryngii crude polysaccharide
[0028] Cut fresh Pleurotus eryngii fruit bodies into 1cm thin slices, dry at 100°C for 30min, after cooling, pour them into a traditional Chinese medicine grinder, and grind them into 20-mesh powder. Put the Pleurotus eryngii powder into a pot, add water and boil for 1 hour at a solid-to-liquid ratio of 1:20. After cooling, the slag-containing extract was divided into centrifuge tubes, and centrifuged at 8000 r / min for 2 min to remove solid residues and obtain a clear water extract of Pleurotus eryngii.
[0029] (2) Preparation of immobilized protease
[0030] Take a 100ml beaker, weigh 2g of chitosan powder, add 20ml of 1% (v / v) glutaraldehyde solution, put it into a magnetic stirrer, stir in a water bath magnetic stirrer at 30°C for 2h, filter to remove glutaraldehyde Dialdehyde solution, mix glutaraldehyde-activated chitosan with 5ml alkaline protease solution (the total enzyme activity is 2000U), stir in a...
Embodiment 2
[0039] (1) Extraction of Pleurotus eryngii crude polysaccharide
[0040] Cut the fresh Pleurotus eryngii mushroom head (that is, the combined part of Pleurotus eryngii and compost) into 1cm thin slices, dry at 100°C for 30min, after cooling, pour it into a traditional Chinese medicine grinder, and grind it into a 100-mesh powder. Put the Pleurotus eryngii powder into a pot, add water and boil for 1 hour at a solid-to-liquid ratio of 1:10. After cooling, the slag-containing extract was divided into centrifuge tubes, and centrifuged at 6000 r / min for 5 min to remove solid residues and obtain a clear water extract of Pleurotus eryngii.
[0041] (2) Preparation of immobilized protease
[0042] Take a 100ml beaker, weigh 2g chitosan powder, add 20ml 1% (v / v) glutaraldehyde solution, put it into a magnetic stirrer, place it in a water bath magnetic stirrer, keep stirring at 30°C for 2h, filter Remove the glutaraldehyde solution, mix glutaraldehyde-activated chitosan with 5ml neutr...
Embodiment 3
[0051] (1) Extraction of Pleurotus eryngii crude polysaccharide
[0052] Cut fresh Pleurotus eryngii and other foreign products into 1cm thin slices, dry at 100°C for 30 minutes, after cooling, pour them into a traditional Chinese medicine grinder, and grind them into 20-mesh powder. Put the Pleurotus eryngii powder into a pot, add water and boil for 1 hour at a solid-to-liquid ratio of 1:30. After cooling, the slag-containing extract was divided into centrifuge tubes, and centrifuged at 5000 r / min for 10 min to remove solid residues and obtain a clear water extract of Pleurotus eryngii.
[0053] (2) Preparation of immobilized protease
[0054] Take a 100ml beaker, weigh 2g of chitosan powder, add 20ml of 1% (v / v) glutaraldehyde solution, put it into a magnetic stirrer, stir in a water bath magnetic stirrer at 30°C for 2h, filter to remove glutaraldehyde Dialdehyde solution, mix glutaraldehyde-activated chitosan with 5ml trypsin solution (total enzyme activity is 2000U), sti...
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