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Fishskin source acellular dermal matrix and preparation method thereof

A technology of decellularized dermis and fish skin, applied in medical science, tissue regeneration, prosthesis, etc., can solve the problems of huge differences in categories and difficulty in obtaining technical inspiration, and achieve simple process, good cell compatibility, and reasonable price Effect

Active Publication Date: 2018-06-22
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, replacing mammals with aquatic organisms has become a research hotspot, and the dermis composition of fish animals is very different from mammals and other phyla, and it is difficult to obtain relevant technical inspiration from other animal phyla in the prior art. Regarding the use of fish skin to prepare Acellular dermal matrix has not been reported in the field of medical materials

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  • Fishskin source acellular dermal matrix and preparation method thereof
  • Fishskin source acellular dermal matrix and preparation method thereof
  • Fishskin source acellular dermal matrix and preparation method thereof

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preparation example Construction

[0037] A preparation method of fish skin source decellularized dermal matrix, comprising the following steps:

[0038] (1) Fish skin pretreatment: scrape off the residual meat on the fish skin, and wash it with sterile phosphate buffer.

[0039] (2) Sterilization and disinfection treatment: wash and soak the fish skin, and carry out sterilization and disinfection treatment to the fish skin.

[0040](3) Degreasing treatment: soak the sterilized fish skin in an alkaline solution for degreasing, and then clean it with sterile phosphate buffer. This process adopts the method of secondary degreasing. On the one hand, the secondary degreasing avoids the loosening of collagen fibers in the fish skin, and on the other hand, it further strengthens the degreasing effect.

[0041] (4) Decolorization treatment: immerse the decolorized fish skin in a decolorizer for decolorization, and then clean it with sterile phosphate buffer. The decolorizing agents are respectively potassium permang...

Embodiment 1

[0048] A preparation method of fish skin source decellularized dermal matrix, comprising the following steps:

[0049] (1) Fish skin pretreatment: scrape off the residual meat on the pangasius skin with a spoon, and wash it three times with 10 mM sterile phosphate buffer solution with a pH of 7.4. The thickness of the fish skin is 0.3mm-1mm.

[0050] (2) Sterilization treatment: soak the fish skin in electrolyzed water with a pH of 6.5 at room temperature for 30 minutes, and clean it with a plasma deionization washing machine.

[0051] (3) Degreasing treatment: soak the fish skin processed through sterilization in 1% (w / v) sodium bicarbonate solution for 24 hours to degrease for the first time. At this time, the solid-liquid ratio of fish skin and sodium bicarbonate solution 1:6 (v / v); then the fish skin is soaked in 1% (w / v) potassium hydroxide solution for 12h to degrease for the second time, at this time, the feed liquid of fish skin and sodium bicarbonate solution The ra...

Embodiment 2

[0058] A preparation method of fish skin source decellularized dermal matrix, comprising the following steps:

[0059] (1) Fish skin pretreatment: scrape off the residual meat on the tilapia skin with a spoon, and wash 6 times with 10 mM sterile phosphate buffer solution with a pH of 7.4.

[0060] (2) Sterilization treatment: Soak the fish skin in electrolyzed water with a pH of 7.5 at room temperature for 20-30 minutes, and clean it with a plasma deionized cleaning machine.

[0061] (3) Degreasing treatment: soak the fish skin processed through sterilization in 3% (w / v) sodium bicarbonate solution for 18h to degrease for the first time. At this time, the solid-liquid ratio of fish skin and sodium bicarbonate solution 1:3 (v / v); then the fish skin is soaked in 0.5% (w / v) potassium hydroxide solution for 18h to degrease for the second time, at this time, the feed liquid of fish skin and sodium bicarbonate solution The ratio was 1:3 (v / v); and then washed 6 times with 10 mM ste...

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Abstract

The invention discloses a method for preparing fishskin source acellular dermal matrix. The method comprises the following steps of (1) fishskin pretreatment; (2) sterilization and disinfection treatment; (3) ungreasing treatment; (4) decoloring treatment; (5) high and low permeability treatment; (6) strong base and ablation treatment, (7) multigelation treatment; and (8) freeze-drying and sizingtreatment. According to the method, osmotic pressure is used to alternatively process lytic cells, at the same time, the combination of strong base ablation and multigelation methods removes cellularconstituents to achieve a more ideal effect of removing cells, the prepared fishskin source acellular dermal matrix has a double-deck structure, the treatment process has little damage on a dermal supporting structure, the integrity of a timbering material can be saved well, and the adhesion and growth of the cells in a later stage is facilitated.

Description

technical field [0001] The invention belongs to the technical field of preparation of biomedical materials, and in particular relates to a decellularized dermal matrix derived from fish skin and a preparation method thereof. Background technique [0002] Tissue engineering is a new interdisciplinary subject emerging in the late 1980s. Scaffold materials used for tissue engineering mainly include natural biological materials and synthetic polymer materials. Acellular dermal matrix is ​​an excellent biomedical material, which has been widely used in the department of skin burns. Later, people tried to apply it to oral repair, covering bone defect wounds, and found that the surface can heal well after a few weeks . Acellular dermal matrix is ​​a natural extracellular matrix that removes epidermal keratinocytes and dermal fibroblasts from donor skin through specific physical and chemical treatments. It has good biocompatibility and strong ability to induce bone regeneration. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/60A61L27/50A61L27/56
CPCA61L27/362A61L27/50A61L27/56A61L27/60A61L2430/40
Inventor 侯虎李八方王月坤
Owner OCEAN UNIV OF CHINA
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