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A kind of decellularized dermal matrix derived from fish skin and its preparation method

An acellular dermis, fish skin technology, applied in medical science, tissue regeneration, prosthesis and other directions, can solve the problems of huge differences in categories, difficult to obtain technical inspiration, etc., to achieve simple process, good cell compatibility, good integrity sexual effect

Active Publication Date: 2021-05-07
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, replacing mammals with aquatic organisms has become a research hotspot, and the dermis composition of fish animals is very different from mammals and other phyla, and it is difficult to obtain relevant technical inspiration from other animal phyla in the prior art. Regarding the use of fish skin to prepare Acellular dermal matrix has not been reported in the field of medical materials

Method used

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  • A kind of decellularized dermal matrix derived from fish skin and its preparation method
  • A kind of decellularized dermal matrix derived from fish skin and its preparation method
  • A kind of decellularized dermal matrix derived from fish skin and its preparation method

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preparation example Construction

[0037] A preparation method of fish skin source decellularized dermal matrix, comprising the following steps:

[0038] (1) Fish skin pretreatment: scrape off the residual meat on the fish skin, and wash it with sterile phosphate buffer.

[0039] (2) Sterilization and disinfection treatment: wash and soak the fish skin, and carry out sterilization and disinfection treatment to the fish skin.

[0040](3) Degreasing treatment: soak the sterilized fish skin in an alkaline solution for degreasing, and then clean it with sterile phosphate buffer. This process adopts the method of secondary degreasing. On the one hand, the secondary degreasing avoids the loosening of collagen fibers in the fish skin, and on the other hand, it further strengthens the degreasing effect.

[0041] (4) Decolorization treatment: immerse the decolorized fish skin in a decolorizer for decolorization, and then clean it with sterile phosphate buffer. The decolorizing agents are respectively potassium permang...

Embodiment 1

[0048] A preparation method of fish skin source decellularized dermal matrix, comprising the following steps:

[0049] (1) Fish skin pretreatment: scrape off the residual meat on the pangasius skin with a spoon, and wash it three times with 10 mM sterile phosphate buffer solution with a pH of 7.4. The thickness of the fish skin is 0.3mm-1mm.

[0050] (2) Sterilization treatment: soak the fish skin in electrolyzed water with a pH of 6.5 at room temperature for 30 minutes, and clean it with a plasma deionization washing machine.

[0051] (3) Degreasing treatment: soak the fish skin processed through sterilization in 1% (w / v) sodium bicarbonate solution for 24 hours to degrease for the first time. At this time, the solid-liquid ratio of fish skin and sodium bicarbonate solution 1:6 (v / v); then the fish skin is soaked in 1% (w / v) potassium hydroxide solution for 12h to degrease for the second time, at this time, the feed liquid of fish skin and sodium bicarbonate solution The ra...

Embodiment 2

[0058] A preparation method of fish skin source decellularized dermal matrix, comprising the following steps:

[0059] (1) Fish skin pretreatment: scrape off the residual meat on the tilapia skin with a spoon, and wash 6 times with 10 mM sterile phosphate buffer solution with a pH of 7.4.

[0060] (2) Sterilization treatment: Soak the fish skin in electrolyzed water with a pH of 7.5 at room temperature for 20-30 minutes, and clean it with a plasma deionized cleaning machine.

[0061] (3) Degreasing treatment: soak the fish skin processed through sterilization in 3% (w / v) sodium bicarbonate solution for 18h to degrease for the first time. At this time, the solid-liquid ratio of fish skin and sodium bicarbonate solution 1:3 (v / v); then the fish skin is soaked in 0.5% (w / v) potassium hydroxide solution for 18h to degrease for the second time, at this time, the feed liquid of fish skin and sodium bicarbonate solution The ratio was 1:3 (v / v); and then washed 6 times with 10 mM ste...

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Abstract

The invention discloses a preparation method of fish skin-derived decellularized dermal matrix, which comprises the following steps: (1) fish skin pretreatment; (2) sterilization treatment; (3) degreasing treatment; (4) decolorization treatment; (5) High and low osmotic treatment; (6) strong alkali erosion treatment; (7) repeated freeze-thaw treatment; (8) freeze-drying and shaping treatment. The present invention uses osmotic pressure to alternately treat and lyse cells, and at the same time cooperates with strong alkali erosion and repeated freezing and thawing methods to remove cell components, and achieves a relatively ideal effect of removing cells. The fish skin-derived acellular dermal matrix prepared by the present invention has Double-layer structure, and the treatment process has little damage to the scaffold structure of the dermis, which can well preserve the integrity of the scaffold material and is conducive to the adhesion and growth of cells in the later stage.

Description

technical field [0001] The invention belongs to the technical field of preparation of biomedical materials, and in particular relates to a decellularized dermal matrix derived from fish skin and a preparation method thereof. Background technique [0002] Tissue engineering is a new interdisciplinary subject emerging in the late 1980s. Scaffold materials used for tissue engineering mainly include natural biological materials and synthetic polymer materials. Acellular dermal matrix is ​​an excellent biomedical material, which has been widely used in the department of skin burns. Later, people tried to apply it to oral repair, covering bone defect wounds, and found that the surface can heal well after a few weeks . Acellular dermal matrix is ​​a natural extracellular matrix that removes epidermal keratinocytes and dermal fibroblasts from donor skin through specific physical and chemical treatments. It has good biocompatibility and strong ability to induce bone regeneration. T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/60A61L27/50A61L27/56
CPCA61L27/362A61L27/50A61L27/56A61L27/60A61L2430/40
Inventor 侯虎李八方王月坤
Owner OCEAN UNIV OF CHINA
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