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A method for localizing the substantia nigra region of the non-human primate brain

A primate and positioning method technology, applied in the field of precise positioning of the substantia nigra of the brain of non-human primates, can solve problems such as difficulty in accurately locating the substantia nigra of the brain and large individual differences of non-human primates , to achieve the effect of reducing the cost of the experiment and using it efficiently

Active Publication Date: 2020-12-01
GUANGDONG LAB ANIMALS MONITORING INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to: overcome the problem in the prior art that it is difficult to accurately locate the substantia nigra region due to the large individual differences of non-human primates, and provide a method for obtaining the substantia nigra region of the non-human primate brain using nuclear magnetic resonance photos. A method of positioning coordinates to achieve precise localization of the substantia nigra region of the nonhuman primate brain

Method used

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  • A method for localizing the substantia nigra region of the non-human primate brain
  • A method for localizing the substantia nigra region of the non-human primate brain
  • A method for localizing the substantia nigra region of the non-human primate brain

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Embodiment

[0027] 1) After the monkey is anesthetized, place the monkey on the MRI machine in a flat position, and use a towel to raise the monkey's head so that its face is facing straight up, and the MRI scan can be started.

[0028] 2) Use the RadiAnt DICOM Viewer software to open the obtained nuclear magnetic resonance photos, and select the photos in the middle of the sagittal plane of the monkey brain (such as figure 1 shown), select the measurement tool of RadiAnt DICOM Viewer software, and pass the monkey's brow center (see figure 2 left arrow) and inion (see figure 2 right arrow) to draw a reference line (see figure 2 ), it has been confirmed through many experiments that the reference line is the horizontal line that the monkey places behind the brain problem locator. According to the location of the substantia nigra in the anatomical atlas ( image 3 ), perpendicular to this baseline, determine the area from the dura vertex to the substantia nigra ( Figure 4 arrow) dis...

experiment example 1～3

[0031] According to the positioning method of the embodiment of the present invention, we carried out the glycerol injection test in the substantia nigra region of some monkeys (glycerol diffuses slowly, which is convenient for re-scanning and confirming by magnetic resonance after injection, and the injected liquid may diffuse faster in actual use. and the injection volume is small, so MRI cannot be used for scan confirmation).

[0032] 1) No. 1 monkey uses the embodiment positioning method to determine X (3.98mm from the midline of the brain), Y (2.91cm from the dura mater perpendicular to the horizontal line in the substantia nigra), and Z coordinates (the center of the eyebrows is perpendicular to the dura mater) after the monkey brain is scanned by magnetic resonance The Y-axis distance is 5.06 cm). According to the positioning method, after the monkey was anesthetized, the substantia nigra region was positioned and injected with glycerol on the brain stereotaxic instrumen...

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Abstract

The invention discloses a localization method of a non-human primate substantia nigra area. According to the localization method, localization coordinates of the non-human primate substantia nigra area are acquired via nuclear magnetic resonance pictures, so that accurate localization of the non-human primate substantia nigra area can be achieved. The method provided by the invent, in comparison with an existing extensive localization method which is implemented based on experiences, is more accurate and reliable and has the advantages of being efficient and being capable of saving experimental cost.

Description

technical field [0001] The invention belongs to the technical field of animal brain structure positioning. More specifically, the invention relates to obtaining the positioning coordinates of the substantia nigra of the brain of non-human primates by using nuclear magnetic resonance photos, so as to realize the location of the substantia nigra of the brain of non-human primates. accurate locating. Background technique [0002] The precise injection technique in the substantia nigra is a key technology for Parkinson's disease model and drug evaluation research. In rodents (mice and rats), through the brain localization map and brain stereotaxic, it is possible to precisely inject virus or non-viral vectors into the substantia nigra region of the brain, thereby successfully constructing an animal model with clinical and pathological characteristics of Parkinson's disease. At the same time, this technology can also be used in Parkinson's gene therapy and stem cell therapy rese...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61B90/10A61B5/055
CPCA61B5/055A61B90/10
Inventor 李舸关雅伦李韵峰刘书华黄韧张钰
Owner GUANGDONG LAB ANIMALS MONITORING INST
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