Method for determining content of toddalolactone in shinyleaf pricklyash root traditional Chinese medicine toothpaste by RP-HPLC (reversed-phase high-performance liquid chromatography)

Abstract

The invention discloses a method for determining the content of toddalolactone in shinyleaf pricklyash root traditional Chinese medicine toothpaste by RP-HPLC (reversed-phase high-performance liquid chromatography). The method has the advantages of simplicity and convenience in operation, rapidness, accuracy, less interference, good precision and repeatability and the like. The invention belongs to the field of chemical testing. The method adopts ethanol to extract toddalolactone in shinyleaf pricklyash root toothpaste, and collects ethanol extract; the RP-HPLC method is adopted to determine the content of toddalolactone in the ethanol extract. The method can provide an experimental basis for the determination of the content of toddalolactone in shinyleaf pricklyash root traditional Chinese medicine toothpaste.

Description

technical field [0001] The invention belongs to the field of chemical detection, in particular to a RP-HPLC method for measuring the content of lianmianzhen in liangmianzhen traditional Chinese medicine toothpaste. Background technique [0002] Liangmianzhen is the dried root of Zanthoxylum nitidum (Roxb.) DC., a Rutaceae plant. It can promote blood circulation and remove blood stasis, promote qi and relieve pain, dispel wind and collaterals, detoxify and reduce swelling. Do not overdose [1] , the variant of Maoliangmianzhen [2] , LMZ medicinal materials are different from Mao LMZ in terms of chemical composition and content. Maoliangzhensu, also known as Feilongzhang blood lactone, is a unique component of Maoliangzhen and Feilongzhang Xuegen; Lianmianzhen chloride is one of the main active ingredients of Liangmianzhen; it exists in medicinal materials Liangmianzhen, Maoliangmianzhen and Feilongzhang in the blood, but the content is different among the three [3-5] . "Ch...

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Problems solved by technology

[0006] At present, the quality control indicators of LMZ have not been perfected, and the quality of LMZ herbs used in the preparation of LMZ toothpaste may vary. However, the formulation process of LMZ toothpaste is strictly kept secret, and there is no relevant report on its quality standard. Therefore, the selection of the raw material drug LMZ will also affect the content and authenticity of the effective components of LMZ toothpaste.

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