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Application of mushroom dietary fiber extracts for preparing pharmaceutical preparation for treating and/or preventing diseases associated with intestinal bacterium community disorder

A technology of intestinal flora imbalance and dietary fiber, applied in food science, function and application of food ingredients, etc., can solve problems such as destroying the balance of intestinal flora, intestinal flora imbalance, changing intestinal flora, etc. Achieve the effects of improving various physiological and pathological indicators related to immunity, alleviating the deterioration of inflammation, and inhibiting the expression of inflammatory factors

Inactive Publication Date: 2018-06-12
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Studies have confirmed that a high-fat diet will change the intestinal flora, reduce the beneficial bacteria, destroy the balance of intestinal flora, and lead to intestinal flora imbalance

Method used

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  • Application of mushroom dietary fiber extracts for preparing pharmaceutical preparation for treating and/or preventing diseases associated with intestinal bacterium community disorder
  • Application of mushroom dietary fiber extracts for preparing pharmaceutical preparation for treating and/or preventing diseases associated with intestinal bacterium community disorder
  • Application of mushroom dietary fiber extracts for preparing pharmaceutical preparation for treating and/or preventing diseases associated with intestinal bacterium community disorder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The preparation method of mushroom dietary fiber extract, the steps are as follows:

[0045] (1) Dried shiitake mushrooms were crushed and weighed. According to the ratio of material to liquid (mass volume ratio) of 1:10, 95% ethanol was used to boil and reflux to extract for 2 hours, and suction filtered to obtain the filter residue;

[0046] (2) Add water to the filter residue, the ratio of material to liquid (mass volume ratio) is 1:10, boil and reflux for extraction for 2 hours;

[0047] (3) When the temperature drops to about 60°C, adjust the pH value to 6-7, add papain for enzymolysis, the enzyme amount is 0.2% (mass percentage) of the sample weight, and enzymolyze in a water bath at 60°C for 1 hour. stop stirring;

[0048] (4) After the enzymolysis is completed, add 95% ethanol in an amount 4 times the volume of the extract after enzymolysis to precipitate overnight, and filter with suction to obtain a filter residue;

[0049] (5) The filter residue is eluted w...

Embodiment 2

[0053] Example 2: Improvement effect of mushroom dietary fiber extract on intestinal tissue damage in high-fat diet-induced obese mice

[0054] 1. Reagents:

[0055] Total cholesterol (T-CHO) test kit: Nanjing Jiancheng Institute of Bioengineering.

[0056] Triglyceride (TG) test kit: Nanjing Jiancheng Institute of Bioengineering.

[0057] Low-density lipoprotein cholesterol (LDL-C) test kit: Nanjing Jiancheng Bioengineering Institute.

[0058] High-density lipoprotein cholesterol (HDL-C) test kit: Nanjing Jiancheng Bioengineering Institute.

[0059] Thirty female C57 mice, SPF grade, weighing 12-14 g, and 28-30 days old, were purchased from Guangdong Medical Experimental Animal Center with license number SCXK (Guangdong) 2013-0002.

[0060] Rats and mice maintained feed: Guangdong Medical Experimental Animal Center, license number SCXK (Guangdong) 2013-0002.

[0061] The high-fat and high-cholesterol feed formula is shown in Table 1:

[0062] Table 1 Main components and ...

Embodiment 3

[0092] Example 3: Improvement effect of mushroom dietary fiber extract on diseases related to intestinal flora imbalance in obese mice induced by high-fat diet

[0093] 1. Experimental method

[0094] The feces of the mice at the end of the animal experiment in Example 2 were collected, quickly frozen with liquid nitrogen, and stored in a -80°C refrigerator. DNA was extracted from stool samples, and the quality of DNA extraction was detected by 0.8% agarose gel electrophoresis, and DNA was quantified by UV spectrophotometer. Forward primer 5'-ACTCCTACGGGAGGCAGCA-3' and reverse primer 5'-GGACTACHVGGGTWTCTAAT-3' were used to amplify the V3-V4 region gene of mouse 16S rRNA. The PCR amplification products were detected by 2% agarose gel electrophoresis, and the target fragments were recovered by gel cutting. Referring to the preliminary quantitative results of electrophoresis, the recovered products of PCR amplification were quantified by fluorescence. The fluorescent reagent wa...

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Abstract

The invention relates to the application of mushroom dietary fiber extracts in the field of pharmaceutics, in particular to the application of the mushroom dietary fiber extracts for preparing a pharmaceutical preparation for treating and / or preventing diseases associated with intestinal bacterium community disorder and a preparation method thereof. The extracts are significantly beneficial to restoration of a dominant bacteria structure in intestines, diversity of bacteria is facilitated, significant treating and / or preventing effects on the diseases associated with an intestinal bacterium community structure are shown, for example, expression of inflammatory factors can be effectively inhibited to effectively relieve the deterioration of inflammation, each item of physiological and pathological indexes related to immune is improved, the extracts can be applied to the fields of medicine, health products and the like, and the extracts have significant economic and social value.

Description

technical field [0001] The present invention relates to the application of the dietary fiber extract of shiitake mushrooms in the field of pharmacy, in particular to the use of the dietary fiber extract of shiitake mushrooms in preparing preparations for treating and / or preventing diseases related to intestinal flora and a preparation method thereof. Background technique [0002] With the improvement of living standards, changes in lifestyle and dietary structure, Westernized diet, increased intake of high-fat food, and insufficient intake of dietary fiber have increased, ignoring the balance of dietary structure, resulting in an increasing number of obese people. Obesity has become one of the public health problems attracting worldwide attention. According to research, the occurrence and development of common diseases in affluent societies are mostly related to obesity, such as diabetes, hypertension, coronary heart disease, liver disease, colon cancer, Alzheimer's disease,...

Claims

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Application Information

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IPC IPC(8): A23L31/00A23L33/21A23L29/00
CPCA23L29/06A23L31/00A23L33/21A23V2002/00A23V2200/30A23V2200/32A23V2200/324A23V2250/208
Inventor 唐晓萃陈地灵杨俭帅欧赖国校谢意珍吴清平杨柏华
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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