Test method of electrochemical sensor

A test method and sensor technology, applied in the field of electrochemical detection, can solve the problems of no signal detected by electrodes, large deviation, and influence on the accuracy of detection results, etc.

Active Publication Date: 2018-06-08
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the existing electrochemical sensor testing methods, due to the different sample injection methods, individual electrodes may not be able to fully absorb the sample due to different sample injection directions, or the electrodes cannot be fully absorbed due to insufficient sample dosage and spread of the sample surface. Filling, or due to multiple intermittent injections, the electrodes cannot be detected or the detected signals are inaccurate, such as three-electrode electronic sensors, due to different injection methods, different doses, reasons for the sample itself, and certain differences in the electronic components of the instrument The characteristics or the detection of environmental temperature and other factors cause the two working electrodes to undergo chemic

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Experimental program
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Embodiment 1

[0034] Embodiment 1 Test method of the present invention

[0035] Add the sample to be tested. The sample to be tested enters the sensor from the top sample inlet of the sensor. First, the sample fills the first electrode and the second electrode (W1 and R), so that a current loop is formed between the first electrode and the second electrode. At this time, the The instrument connected to the sensor will automatically start up under the drive of its internal power-on circuit, the sample to be tested will react with the reagents on the two working electrodes of the electrochemical sensor, and two current signals will be read within 15 to 30 seconds after the instrument starts timing. , corresponding to I 1 and I 2 , according to I 1 / I 2 The ratio of the ratio judges the injection situation. The judging method comprises the steps of: setting a preset parameter range (A, B), when A≤I 1 / I 2 ≤B, it indicates that the injection is correct.

Embodiment 2

[0036] Embodiment 2 Test method of the present invention

[0037] Add the sample to be tested. The sample to be tested enters the sensor from the top sample inlet of the sensor. First, the sample fills the first electrode and the second electrode (W1 and R), so that a current loop is formed between the first electrode and the second electrode. At this time, the The instrument connected to the sensor will automatically start up under the drive of its internal power-on circuit, the sample to be tested will react with the reagents on the two working electrodes of the electrochemical sensor, and two current signals will be read within 15 to 30 seconds after the instrument starts timing. , corresponding to obtain two current values ​​I 1 and I 2 , according to I 1 , I 2 Calculate the current value I to obtain the concentration of the substance to be tested in the sample to be tested;

[0038] Wherein, the calculation method of current value I is: I=a 1 I 1 +b 1 I 2 , 0.5≤a≤...

Embodiment 3

[0039] Embodiment 3 testing method of the present invention

[0040]Add the sample to be tested. The sample to be tested enters the sensor from the top sample inlet of the sensor. First, the sample fills the first electrode and the second electrode (W1 and R), so that a current loop is formed between the first electrode and the second electrode. At this time, the The instrument connected to the sensor will automatically start up under the drive of its internal power-on circuit, the sample to be tested will react with the reagents on the two working electrodes of the electrochemical sensor, and two current signals will be read within 15 to 30 seconds after the instrument starts timing. , corresponding to obtain two current values ​​I 1 and I 2 , according to I 1 , I 2 Calculate the current value I to obtain the concentration of the substance to be tested in the sample to be tested;

[0041] Wherein, the calculation method of current value I is:

[0042] Set the preset para...

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Abstract

The invention relates to the technical field of electrochemical detection, and especially relates to a test method of an electrochemical sensor. The test method can be used to accurately judge whethersample introduction is correct or not during the detection of a sample to be tested by adopting the electrochemical sensor, can significantly reduce a deviation between a detection value and a real value, allows the measured value to be accurate and reliable, and effectively solves the problem of inaccurate detection of existing detection methods.

Description

technical field [0001] The invention relates to the technical field of electrochemical detection, in particular to a testing method for an electrochemical sensor. Background technique [0002] Electrochemical sensors (test strips) have been used to detect or measure the presence of a certain substance in a fluid sample, or the concentration level of the substance, and the test results are used as a health reference or a basis for disease diagnosis. An electrochemical sensor includes a reagent mixture comprising at least one electron transfer agent (also referred to as an "electron mediator") and an analyte-specific biocatalytic protein (eg, a particular enzyme) and one or more electrodes. Such sensors rely on the transfer of electrons between the electronic mediator and the electrode surface and function by measuring electrochemical redox reactions. In an electrochemical biosensor system or device, an electrical signal related to the concentration of the analyte being measu...

Claims

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Application Information

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IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 蔡晓华胥理铭萱王朋书沈良田时雨
Owner SINOCARE
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