Substrate for detecting beta-D-glucuronidase and preparation method thereof as well as kit
A technology of aldolidase and glucuronic acid, which is applied to the preparation of sugar derivatives, biochemical equipment and methods, sugar derivatives, etc., can solve the problems of insensitivity to color development, difficulty in synthesis, and high price, and achieve The effect of cost reduction and simple synthesis process
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[0022] The present invention is illustrated below through specific examples, but the present invention is not limited.
[0023] 1. Synthesis of D-methyl glucuronate. It can be represented by the following reaction formula:
[0024]
[0025] The specific preparation steps are as follows: D-glucuronolactone (1) (17.6g, 0.1mol) was dissolved in 200ml methanol and cooled to 0°C. NaOH (100 mg) was added and stirred for 2 hours. The reaction was concentrated to dryness to give methyl D-glucuronate (2) as a white solid (yield 20.8 g, yield 100%).
[0026] 2. Synthesis of 1,2,3,4-tetra-O-acetyl-D-glucuronic acid methyl ester. It can be represented by the following reaction formula:
[0027]
[0028] The specific preparation steps are as follows: D-methyl glucuronate (2) (20.8g, 0.1mol) was dissolved in pyridine (45ml) and cooled to below 5°C, then acetic anhydride (54mL, 0.6mol) was added dropwise to keep it stable Not exceeding 10°C, then stirred at room temperature for 3 ...
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