Truncated e2 protein of swine fever virus and its application

Abstract

The invention discloses a hog cholera virus truncated E2 protein which is designed on the basis of protein spatial structure, and an application of the same. In the invention, according to the crystalstructure of bovine viral diarrhea virus E2 protein, the spatial structure of the hog cholera virus E2 protein is simulated, and then the hog cholera virus E2 protein is subjected to truncated expression, wherein the amino acid sequence of the truncated protein E2B / C / D / A is represented as the SEQ ID No.1. The truncated protein can maintain the complete antigenicity of the E2 protein, and has no cross reaction with a bovine viral diarrhea virus antibody. The invention further constructs a CHO cell line which stably expresses the truncated protein E2B / C / D / A and is assigned the accession numberof CGMCC No.14722. The invention also discloses an indirect ELISA kit which is used for detection of a hog cholera virus antibody, wherein the enveloped antigen is the hog cholera virus truncated protein E2B / C / D / A. The kit is used for specifically detecting the hog cholera virus antibody with high specificity, sensitivity and repeatability.

Description

technical field [0001] The invention relates to the truncated E2 protein of the classical swine fever virus, and also relates to the application of the truncated E2 protein of the classical swine fever virus in detecting the antibody of the classical swine fever virus, and belongs to the field of expression and application of the truncated E2 protein of the classical swine fever virus. Background technique [0002] Classical swine fever (CSF) is a highly contagious disease caused by classical swine fever virus (CSFV), which seriously endangers the global swine industry. CSFV is a member of the Flaviviridae family and the Pestivirus genus. Other members of the Pestivirus genus include bovine viral diarrhea virus (BVDV) and border disease virus (BDV). BVDV can infect not only cattle, but also pigs. CSFV is an enveloped single-stranded positive-sense RNA virus with a genome length of 12.3kb. The genome contains a large open reading frame, encoding a polyprotein of about 3,90...

AI Technical Summary

Problems solved by technology

In addition, at present, China mainly prevents and controls swine fever by widely inoculating the attenuated swine fever rabbit vaccine (C strain). Fetal bovine serum is used in the production of live cell-derived swine fever vaccine. However, due to the lack of effective monitoring, many BVDV-contaminated Fetal bovine serum is used in the production of classical swine fever vaccine, resulting in the production of BVDV antibodies in swine fever vaccinated pigs (Xu Guodong. Pollution status of bovine viral diarrhea in domestic pig vaccines. China Animal Health. 2017b,19(4):5–8 .), thereby interfering with the detection of CSFV antibodies (Lin GZ, Zheng FY, Zhou JZ, Cao XA, Gong XW, Wang GH, Qiu CQ. An indirect ELISA of classical swine fever virus based on quadruple antigenic epitope peptide expressed in E.coli. Virol Sin.2010,25(1):71–6.)

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