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Method for detecting flavonoids component in peony petals

A technology of peony petals and flavonoids is applied in the research field of peony petal components, which can solve the problems of long operation time, low accuracy and insufficient sensitivity, and achieve the effects of simple operation steps, high precision and high sensitivity.

Inactive Publication Date: 2018-06-01
城发投资集团有限公司 +1
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Problems solved by technology

However, probably due to the complex composition of flavonoids contained in peony petals, the accurate and complete identification of its chemical composition has become a challenge for researchers
[0004] In the prior art, ultraviolet-visible spectrophotometer (UV), thin-layer chromatography (TLC) and high-performance liquid chromatography-diode array detector (HPLC-DAD) were used to analyze the flavonoid components of different varieties of peony petals. analysis, but the extraction steps of flavonoids in this method are complicated, and the thin-layer chromatography method used is cumbersome to operate but the accuracy is not high; the flavonoids of peony petals determined by this method are limited (see: Sun Zefei. Analysis of flower flavonoid components and antioxidant capacity [D]. Northwest A&F University, 2015.)
In addition, ultra-high performance liquid chromatography-diode array detector (UPLC-PDA) and ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS) have been used in the prior art to detect The flavonoid components in seven kinds of peony petals were qualitatively and quantitatively analyzed, but the extraction steps of flavonoid substances in this method were complicated and the operation time was long. In addition, the chromatographic conditions and mass spectrometry conditions in this method were not optimized enough to detect flavonoid components in peony petals. When the amount of flavonoids was high but the sensitivity was not enough, only 15 types of flavonoids in peony petals were detected, which was not comprehensive enough (see: Zhang Baozhi, Hu Yonghong, Han Jigang, et al. Analysis of flavonoids in petals of seven Jiangnan peony varieties[J] .Northern Horticulture, 2013(2):61-65.)
[0005] At present, many precious components of flavonoids in peony petals have not been detected by existing technologies, and the method for the most comprehensive detection of flavonoids in peony petals has not been reported

Method used

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  • Method for detecting flavonoids component in peony petals
  • Method for detecting flavonoids component in peony petals
  • Method for detecting flavonoids component in peony petals

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Experimental program
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preparation example Construction

[0031] (1) Preparation of flavonoid extract: grind peony petals under freezing conditions into peony petal powder, mix the peony petal powder with alcohol solution to obtain a mixture containing peony petals, and then carry out the mixture in sequence Ultrasonic treatment and centrifugation, collecting the supernatant and removing the precipitate, and then filtering the collected supernatant through a filter membrane to remove solid impurities to obtain a flavonoid extract;

[0032] (2) The chromatographic conditions of the ultra-high performance liquid chromatography, the mass spectrometry conditions of the triple quadrupole time-of-flight tandem mass spectrometer and the setting of the detection wavelength of the diode array detector;

[0033](3) Using ultra-high performance liquid chromatography-diode array detector-triple quadrupole time-of-flight tandem mass spectrometry to detect the flavonoid extract, and obtain the detection results; and

[0034] (4) Identifying the st...

Embodiment 1

[0050] Grind 15 parts of fresh peony petals into peony petal powder under liquid nitrogen, weigh 300 mg, add 1% formic acid by volume, 900 μL of methanol solution with a volume concentration of 80%, extract the flavonoid components in peony petals, and vortex for 1 min Mix well, ultrasonically extract for 30 minutes, and centrifuge at a high speed of 13000r / min for 10 minutes, collect the supernatant and remove the precipitate, then pass the supernatant through a 0.22 μm organic filter to remove solid impurities to obtain flavonoid extract. The obtained flavonoid extract was analyzed by ultra-high performance liquid chromatography-diode array detector-triple quadrupole time-of-flight tandem mass spectrometry. Wherein, the settings of chromatographic conditions, mass spectrometry conditions and diode array detector detection wavelength range are as follows:

[0051] The chromatographic conditions are: mobile phase: solvent A is a mixed solution of acetonitrile and water contain...

Embodiment 2

[0071] Embodiment 2 is basically the same as Embodiment 1, the difference is:

[0072] The chromatographic conditions are: mobile phase: solvent A is a mixed solution of acetonitrile and water containing 0.2% volume formic acid, the volume ratio of water and acetonitrile is 90:10, solvent B is an acetonitrile solution containing 0.2% volume formic acid; flow rate is 0.2mL / min; column temperature is 30°C; injection volume is 4μL; gradient elution conditions: 0-22min, 100% solvent A-72% solvent A, 0% solvent B-28% solvent B; 22-22.5min, 72% solvent A~60% solvent A, 28% solvent B~40% solvent B; 22.5~23min, 60% solvent A~0% solvent A, 40% solvent B~100% solvent B; 23~26.5min, 0 % solvent A, 100% solvent B; 26.5 ~ 27min, 0% solvent A ~ 100% solvent A, 100% solvent B ~ 0% solvent B; 27 ~ 32min, 100% solvent A, 0% solvent B, that is, liquid The gradient elution conditions of phase chromatography were set according to the procedures in Table 1.

[0073] A total of 5 main anthocyani...

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Abstract

The invention relates to a method for detecting a flavonoids component in peony petals. The method comprises the following steps: preparing a flavonoids extracting solution; setting a chromatographiccondition, a mass spectrum condition and a detection wavelength of a diode array detector; detecting the flavonoids extracting solution by adopting an ultra performance liquid chromatograph-diode array detector-triple quadrupole flight time tandem mass spectrometry and obtaining a detection result; carrying out structure identification on a flavonoids substance through analyzing the detection result; reckoning the structure of the flavonoids component. The method provided by the invention has the advantages of simple operation steps, rapid detection speed and high accuracy and sensitivity andcan be used for detecting the flavonoids component with the extremely less content; identification species are comprehensive and 67 types of main flavone, flavonol, flavanone and flavanol compounds and 5 types of main anthocyanin compounds are totally identified from the peony petals; the blank of identifying the flavonoids component in the peony petals is filled up and the method has important meanings on in analysis of nutrient components of the peony petals with different varieties.

Description

technical field [0001] The invention relates to a method for researching peony petal components, in particular to a method for detecting flavonoid components in peony petals by using ultra-high performance liquid chromatography-diode array detector-triple quadrupole time-of-flight tandem mass spectrometry. Background technique [0002] Flavonoids refer to compounds whose basic core is 2-phenylchromone, that is, a series of compounds in which two benzene rings with phenolic hydroxyl groups are connected to each other through the central three carbon atoms. According to the basic molecular structure and conformation of flavonoids, flavonoids are generally divided into six categories: flavonoids, flavonols, isoflavones, flavanones, flavanols and anthocyanins. Flavonoids, also known as bioflavonoids, are the most abundant polyphenolic compounds in the human diet. They are widely found in vegetables, fruits, tea and grapes. They have significant effects on the three main stages o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 杨德顺张秀新刘永森薛璟祺王顺利张双峰范俊峰
Owner 城发投资集团有限公司
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