Cultivation method for acquiring virus-free seedlings of anthurium andraeanum lind by combining thermal treatment and stem-tip stripping

Abstract

The invention discloses a cultivation method for acquiring virus-free seedlings of anthurium andraeanum lind by combining thermal treatment and stem-tip stripping. The method comprises the following steps of explant treatment; callus induction and proliferation; adventitious bud induction and proliferation; adventitious bud variable temperature heat treatment; stem-tip stripping; adventitious buddifferentiation and proliferation of stem tip callus; virus detection; acquisition of a virus-free adventitious bud material; and acquisition of virus-free seedlings. The method has the beneficial effects that compared with the treatment method directly performing thermal treatment but avoiding stem-tip stripping, the method can obtain the virus-free seedlings with higher virus elimination rate; and compared with the method of directly acquiring a stem tip explant from a potted plant and cultivating the virus-free seedling, the method has the advantage of providing thousands of explants for stem-tip stripping through one plant, and also has the characteristics of low pollution rate, low cost, no damage to a mother plant offering the explant and the like. Through adoption of the cultivationmethod for acquiring virus-free seedlings of anthurium andraeanum lind by combining thermal treatment and stem-tip stripping, a large quantity of strong virus-free seedlings with high virus elimination efficiency can be acquired within a short time, so that the method provides a technology basis for mass reproduction of virus-free seedlings of anthurium andraeanum lind.

Description

technical field [0001] The invention relates to the technical field of plant detoxification and tissue culture, in particular to a cultivation method for obtaining anthuria anthurium virus-free seedlings through heat treatment combined with stem tip stripping. Background technique [0002] Anthurium (Anthuium andraeanum Lind) is a perennial evergreen herb of Araceae, also known as Anthurium, Anthurium, Anthurium, etc. Its flower shape is unique and its color is gorgeous. It is one of the high-end flowers in the flower market and a high-end flower for important occasions. The preferred type of flower decoration and cut flowers, with high ornamental value and economic value, has become the second largest commercial flower in the world after tropical orchid. [0003] Viruses are one of the main problems that threaten plant quality and yield. At present, there is still no specific control drug, and due to virus infection, the yield and quality of many crops or flowers continue t...

AI Technical Summary

Problems solved by technology

However, a flower or plug seedling of Anthurium has only one stem tip. If it is directly used as an explant for stem tip stripping, one plant provides a stem tip. If it is really necessary to obtain a virus-free seedling, it will take several The stem tips of ten or even hundreds of Anthurium plants are used as explants, which virtually brings difficulties to the development of Anthurium stem tip detoxification technology. At the same time, the high cost also discourages researchers and seriously restricts The development process of industrialized breeding of anthuria anthurium seedlings free of virus

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