A system for determining the haplotype of the fetal alpha thalassemia gene
A thalassemia and haplotype technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of the small number of detection sites, the difficulty of sampling, the inability to detect maternal mutations, and the required sample size. Achieve the effect of simple sample, avoid bleeding and strong practicability
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Embodiment 1
[0172] 1. Prepare the chip for alpha thalassemia gene hybridization capture
[0173] Determine the region where the common alpha thalassaemia is located, and the preferred region is the region of alpha thalassemia on chromosome 16 from 60001 to 434700.
[0174] To remove repeated fragments, the preferred method is to remove regions with more than 100 repeated fragments, that is, if a certain fragment can be compared to more than 100 regions on the chromosome, then remove these fragments.
[0175] To obtain a chip for thalassemia hybridization capture, the preferred method is a liquid phase capture chip.
[0176] At the same time, the PCR method can be used to amplify and enrich the long fragments, and the PCR method can be used as a supplementary method to assist the chip in capturing the target region.
[0177] 2. Construction of the third-generation library
[0178] a. Sample preparation: Obtain blood samples from the fetal father and mother, and extract whole-genome DNA f...
Embodiment 2
[0253] Non-invasive thalassemia detection was performed on 1 patient, the father and mother of this case were both -- sea Carrier of the mutation, the fetus is-- sea homozygous mutation.
[0254] 1. Collection and processing of father and mother samples
[0255] Using Streck blood collection tubes, 5 mL of peripheral blood was collected from the father and mother according to the standard peripheral blood collection operation. After the collection, the peripheral blood of the father and mother was separated in time according to the standard two-step centrifugation method.
[0256] 1.1 DNA extraction from plasma
[0257] The TIANamp Micro DNA Kit was used to extract the free DNA from the mother's peripheral blood plasma. The specific operation steps are as follows:
[0258] 1.1.1 Take 600 μL of pregnant women’s peripheral blood plasma into a 2 mL centrifuge tube, add 20 μL of Proteinase K solution, vortex fully, and centrifuge briefly.
[0259] 1.1.2 Add 600 μL of buffer s...
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