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A kind of fermentation process of bacillus subtilis

A technology of Bacillus subtilis and fermentation process, applied in the field of Bacillus subtilis, can solve the problems that the product quality is difficult to meet the standard, the Bacillus is susceptible to the infection of miscellaneous bacteria, and the composition of raw materials is complicated, so as to reduce the pollution of miscellaneous bacteria and inhibit miscellaneous bacteria. the resulting effect of

Inactive Publication Date: 2020-05-26
北京中科润之农业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of fermentation on solid medium, due to the complex composition of raw materials, Bacillus is easily infected by bacteria after multi-stage culture and open fermentation, and the product quality is difficult to reach the standard

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] S1. Inoculate Bacillus subtilis into 25 kg of liquid medium, adjust the pH of the liquid medium to 7.5, and cultivate it under aerobic conditions at 32°C for 15 hours to become first-class seeds; wherein, the liquid medium includes 0.2 parts of magnesium sulfate, sulfuric acid 2 parts ammonium, 1 part sodium citrate, 6 parts potassium dihydrogen phosphate, 4 parts dipotassium hydrogen phosphate, 10 parts peptone, 1 part sodium chloride, 1 part potassium dihydrogen phosphate;

[0026] S2. Inoculate the primary seeds into 35 kg of liquid medium, adjust the pH of the liquid medium to 7.5, and cultivate them under aerobic conditions at 32°C for 28 hours to become secondary seeds;

[0027] S3. Inoculate the secondary seeds in 25 kg of liquid medium and cultivate them. Under the aerobic condition of 32° C., cultivate them for 33 hours by means of continuous fed-batch fermentation.

[0028] S4. Inoculate the tertiary seeds on the solid medium, ferment and cultivate for 33 hour...

Embodiment 2

[0031] S1. Inoculate Bacillus subtilis into 20 kg of liquid medium, adjust the pH of the liquid medium to 7, and cultivate it under aerobic conditions at 28°C for 18 hours to become first-class seeds; wherein, the liquid medium includes 0.2 parts of magnesium sulfate, sulfuric acid 2 parts ammonium, 1 part sodium citrate, 6 parts potassium dihydrogen phosphate, 4 parts dipotassium hydrogen phosphate, 10 parts peptone, 1 part sodium chloride, 1 part potassium dihydrogen phosphate;

[0032] S2. Inoculate the primary seeds into 40 kg of liquid medium, adjust the pH of the liquid medium to 7, and cultivate them under aerobic conditions at 32°C for 32 hours to become secondary seeds;

[0033] S3. Inoculate the secondary seeds in 20 kg of liquid medium and cultivate them. Under the condition of aerobic conditions at 28° C., cultivate them for 32 hours by means of continuous feeding and batch fermentation.

[0034] S4. Inoculate the tertiary seeds on the solid medium, ferment and cul...

Embodiment 3

[0037] S1. Inoculate Bacillus subtilis into 30 kg of liquid medium, adjust the pH of the liquid medium to 7, and cultivate it under aerobic conditions at 28°C for 18 hours to become first-class seeds; wherein, the liquid medium includes 0.2 parts of magnesium sulfate, sulfuric acid 2 parts ammonium, 1 part sodium citrate, 6 parts potassium dihydrogen phosphate, 4 parts dipotassium hydrogen phosphate, 10 parts peptone, 1 part sodium chloride, 1 part potassium dihydrogen phosphate;

[0038] S2. Inoculate the primary seeds into 30 kg of liquid medium, adjust the pH of the liquid medium to 8, and cultivate them under aerobic conditions at 36° C. for 32 hours to become secondary seeds;

[0039] S3. Inoculate the secondary seeds in 20 kg of liquid medium and cultivate them. Under the aerobic condition of 36° C., cultivate them for 32 hours by means of continuous fed-batch fermentation.

[0040] S4. Inoculate the tertiary seeds on the solid medium, ferment and cultivate for 36 hours,...

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Abstract

The invention relates to a fermenting technology of bacillus subtilis, and belongs to the technical field of bacillus subtilis, which aims at solving the problem of multiple harmful microbes in the bacillus subtilis fermenting process. The fermenting technology of the bacillus subtilis comprises the following steps of culturing of primary seeds, culturing of secondary seeds, culturing of tertiaryseeds, and generating of a fermenting product, wherein a solid culture medium is prepared from the following raw materials of straw, corn meal, wheat bran, diethylaminoethyl crosslinked sephadex, 1-(2-(beta-D-glucopyranose oxy)-4,6-dihydroxy phenyl)-3-(4-hydroxyphenyl)-acetone, and D-2,3,5-6-tetrahydroxy-2-hexenic acid-gamma-lactone. The fermenting technology has the advantages that the pollutionby the harmful microbes is greatly decreased, the property of the prepared bacillus subtilis product is greatly improved, the function of promoting the growth of chicks is good, and the bacillus subtilis product is a feed additive with biological activity.

Description

technical field [0001] The invention relates to the field of bacillus subtilis, more specifically, it relates to a fermentation process of bacillus subtilis. Background technique [0002] Drug feed additives, especially the use of antibiotic feed additives, play an important role in animal disease prevention and treatment, but at the same time, it also has a certain impact on animal husbandry production and human beings. Long-term use of antibiotics will cause Animals have developed drug resistance and endogenous infections, which have gradually limited the application of antibiotics. [0003] Microbial feed additives are live bacteria products containing beneficial microorganisms to animals developed according to the theory of microecology. They volatilize by maintaining the balance of microorganisms in the intestinal tract of animals, and have various functions such as promoting animal growth and development, and improving animal body immunity. And no pollution, no residu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/125
CPCA23K10/18C12N1/20
Inventor 张伶燕李卫军于洪恩
Owner 北京中科润之农业科技发展有限公司
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