A kind of bioactive polypeptide vpitptln and its preparation method and application

A bioactive peptide and drug technology, applied in the protein field, can solve problems such as human body risks, and achieve the effects of reducing body aging, improving vitality, and improving anti-aging activity.

Active Publication Date: 2020-07-17
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Some early synthetic antioxidants such as butyl hydroxyanisole (BHA) and 2,6-di-tert-butyl-4-methylphenol (BHT) were used in food as lipid antioxidants, but these artificial Synthetic additives are potentially dangerous to the human body

Method used

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  • A kind of bioactive polypeptide vpitptln and its preparation method and application
  • A kind of bioactive polypeptide vpitptln and its preparation method and application
  • A kind of bioactive polypeptide vpitptln and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The artificial synthesis of embodiment 1 active peptide VPITPTLN

[0039] 1. Synthesis of Bioactive Peptides

[0040] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak in 50ml of dichloromethane (DCM).

[0041] After 2.2 hours, the resin was washed with nitrogen-dimethylformamide (DMF) 3 times the volume of the resin, and then drained. This was repeated four times, and the resin was drained for use.

[0042] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0043] 4. Take a small amount of resin and use ninhydrin (Nine Wells Ninhydrin) method to test it (Two drops each of Test A and Test B, react at 100°C for 1 min), if the resin is colored, it means that the deprotection is successful.

[0044] 5. ...

Embodiment 2

[0080] Antioxidant activity experiment of embodiment 2 bioactive peptides

[0081] 1. Determination of in vitro antioxidant activity of biologically active peptide VPITPTLN by [DPPH·] method

[0082] 1. Experimental reagents and instruments:

[0083] Reagent: 1,1-diphenyl-2-trinitrophenylhydrazine (1,1-Diphenyl-2-picrylhydrazyl[DPPH·]), produced by Wako Company of Japan; Methanol, provided by Shanghai Sinopharm Company; obtained in Example 1 Milk-derived bioactive polypeptide VPITPTLN.

[0084] Main instruments: Sunrise microplate reader, product of Tecan Company of Austria; 96-well cell culture plate, manufactured by Millipore Company of the United States; analytical balance, product of Meitelei-tolido Company.

[0085] 2. Experimental method:

[0086] (1) 1mmol / L [DPPH·]methanol solution

[0087] Weigh 0.349 mg [DPPH·] with an analytical balance and dissolve it in 1 mL of methanol solution to prepare a 1 mmol / L [DPPH·] methanol solution, store it in the dark on tin foil,...

Embodiment 3

[0119] Anti-aging activity experiment of embodiment 3 bioactive peptides

[0120] 1. Experiments on the effect of biologically active polypeptide VPITPTLN on the structure of spleen tissue in vivo

[0121] 1. Experimental reagents and instruments:

[0122] Reagents: experimental animal ICR mice (male 5 weeks old), Shanghai Experimental Animal Center; D-gal, Sinopharm Chemical Reagent Co., Ltd.; paraformaldehyde, Sinopharm Chemical Reagent Co., Ltd.; sodium chloride, Sinopharm Chemical Reagent Co., Ltd. Co., Ltd.; the milk-derived bioactive polypeptide VPITPTLN obtained in Example 1.

[0123] Instruments and equipment: CM-230 Mole ultra-clean water, Shanghai Moller Scientific Instrument Co., Ltd.; MilliporeMILLEX GP0.22μm filter membrane, Millipore Corporation; GL-22M high-speed refrigerated centrifuge, Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.

[0124] 2. Experimental method:

[0125] (1) Modeling of animal aging models

[0126] After one week of adaptive feeding,...

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Abstract

The invention relates to the field of protein, in particular to a bioactive polypeptide VPITPTLN and a preparing method and application thereof. The amino acid sequence of the bioactive polypeptide VPITPTLN is Val-Pro-Ile-Thr-Pro-Thr-Leu-Asn. Through in-vitro antioxidant experiments and in-vivo anti-aging experiments, it is proved that polypeptide VPITPTLN has great antioxidant biological activities and anti-aging activities. On one hand, the bioactive polypeptide VPITPTLN has a great antioxidant activity and can remove free radicals in the organism and improve the life quality; on the other hand, the vigor of an in-vivo anti-peroxidase system can be improved, the function of the organism of resisting exogenous stimulation is strengthened, thus the probability that the engine gets aged, grows old and gets sick is lowered, and the bioactive polypeptide VPITPTLN is very significant in developing food, healthcare products and drugs with antioxidant functions and anti-aging functions.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active polypeptide VPITPTLN and its preparation method and application. Background technique [0002] During the fermentation process of milk by lactic acid bacteria, a part of protein in milk is metabolized and utilized by lactic acid bacteria, and a series of physiological and biochemical reactions occur, making the protein into polypeptides or free amino acids, which are digested and absorbed by the human body or absorbed and transported by small intestinal epithelial cells directly into the blood circulation of the human body. Among these peptides, some have special physiological functions and are called "bioactive peptides". [0003] It is especially important to find safe bioactive peptides in natural food sources. In recent years, it has been found that some food-derived polypeptides have good biological activity, such as corn short peptides, soybean peptides, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/12A61K38/17A61P37/04A61P39/06A23L33/18A61K8/64A61Q19/08
CPCA23L33/18A23V2002/00A61K8/64A61K38/00A61Q19/08C07K14/4732A23V2250/55A23V2200/302
Inventor 张少辉张伯宇李云飞汪超陈静范梦珠李婉如李阜烁
Owner ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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