Liquid drop micro-fluidic chip used for detecting diabetes with high sensitivity and detection method
A microfluidic chip, high-sensitivity technology, used in medical equipment and microfluidic fields, can solve the problems of large size of detection instruments, low detection sensitivity, complicated operation steps, etc., and achieve small size, good permeability, and simple operation. Effect
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Embodiment 1
[0032] refer to figure 2 , a droplet microfluidic chip for high-sensitivity detection of diabetes in the present embodiment, the material is PMMA, which includes four layers of simple planar structure: cover sheet layer 20, upper fluid channel layer 30, lower fluid channel layer 40, base layer 50. The thickness of the cover sheet layer 20 and the base layer 50 are both 2 mm, the thickness of the upper fluid channel layer 30 and the lower fluid channel layer 40 are both 1 mm, the overall polymer column is about 50 mm long, 0.5 mm wide, and 0.5 mm high; the droplet generator cross Channel height 0.5mm, W 1 =W 2 =W 3 =W 4 =0.2mm, W=0.5mm; the diameter of the micro detection cell is 1mm, and the height is 2mm; the diameters of the water phase inlet 21, the first oil phase inlet 22, the second oil phase inlet 23 and the fluid outlet 24 are all 1.6mm.
[0033] In this example, the preparation process of a strong cation-exchange polymer monolithic column in a droplet microfluidi...
Embodiment 2
[0037] refer to figure 2 , a droplet microfluidic chip for high-sensitivity detection of diabetes in this embodiment, the material is COC, the thickness of the cover layer 20 and the base layer 50 are both 2 mm, the upper fluid channel layer 30 and the lower fluid channel layer The thickness of 40 is 0.5mm, the overall polymer column is about 30mm long, 0.5mm wide, and 0.3mm high; the cross channel of the droplet generator is 0.3mm high, W 1 =W 2 =W 3 =W 4 =0.3mm, W=0.5mm; the diameter of the micro detection cell is 0.6mm, and the height is 1mm; the diameters of the water phase inlet 21, the first oil phase inlet 22, the second oil phase inlet 23 and the fluid outlet 24 are all 1.0mm.
[0038]The preparation process of a strong cation-exchange polymer monolithic column in a droplet microfluidic chip for high-sensitivity detection of diabetes in this example: the strong cation-exchange polymer monolithic column was prepared by thermally induced polymerization. The first ste...
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