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Wheat gene Yr10 molecular marker and application thereof to screening of puccinia striiformis westend.f.sp.tritici-resistant wheat

A molecular marker, stripe rust technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as aggravating the environmental burden and increasing the cost of wheat production

Active Publication Date: 2018-05-08
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the pesticides Zinc, fluorobarium compound, disodium hexafluorosilicate, sodium sulfapyridine, sulfapyridine and Bordeaux mixture to control the spread of wheat stripe rust have played a certain role in the prevention and control of wheat stripe rust, but the increase Increased the cost of wheat production and increased the burden on the environment

Method used

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  • Wheat gene Yr10 molecular marker and application thereof to screening of puccinia striiformis westend.f.sp.tritici-resistant wheat
  • Wheat gene Yr10 molecular marker and application thereof to screening of puccinia striiformis westend.f.sp.tritici-resistant wheat
  • Wheat gene Yr10 molecular marker and application thereof to screening of puccinia striiformis westend.f.sp.tritici-resistant wheat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Genetic mapping and fine mapping of the Yr10 gene. The wheat cultivar Moro carrying the Yr10 gene was crossed with the susceptible cultivar Huixianhong to create a segregation population, and two F strains with a 3:1 segregation of resistance and susceptibility were screened. 3 generation family (F 2 -7 and F 2 -51) Carry out gene mapping. The two families were respectively numbered as Pop8 (58 strains) and Pop10 (81 strains) subpopulations, and 7,500 offspring materials were obtained by using 35 strains separated by disease resistance (F 4 ) constitute the fine-mapped population (Pop11).

[0061] The phenotypic identification of plant materials was performed by field injection inoculation. Take out the spores stored at -20°C, prepare an appropriate amount of water, mix the spores into the water and shake until the spore water suspension turns light orange, put the spore water suspension at 22°C, shake and mix on a shaker at 180rpm for 30min, and use it for injectio...

Embodiment 2

[0067] 1. Selection of materials: The plant materials used in this implementation case are the wheat variety Moro containing the wheat stripe rust resistance Yr10 gene, the wheat variety Huixianhong that does not contain the wheat stripe rust resistance Yr10 gene, and the hybrid F 1 Substitute plants, finally with ddH 2 O was used as a blank control.

[0068] 2. Wheat genomic DNA extraction, the specific method is as follows:

[0069] Using the SDS method to extract the wheat genomic DNA to be detected:

[0070] (1) Take fresh leaves or leaves stored at -80°C ultra-low temperature, put them into a 2mL centrifuge tube, and quickly place them in liquid nitrogen;

[0071] (2) Quickly grind the material in (1) into powder, add 800 μL of DNA extraction solution, shake and mix;

[0072] (3) Add 5 μL of RNaseA with a concentration of 10 mg / mL, warm it in a water bath at 37°C for 30 minutes, and mix it by turning it up and down;

[0073] (4) Add 800 μL of a mixed solution of pheno...

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Abstract

The invention provides a wheat gene Yr10 molecular marker and application thereof to screening of puccinia striiformis westend.f.sp.tritici-resistant wheat and belongs to the technical field of wheatbreeding. The wheat gene Yr10 molecular marker is characterized in that the Yr10 molecular marker has a nucleotide sequence shown as SEQ ID No.1 in a sequence table. The molecular marker is completelylinked with a puccinia striiformis westend.f.sp.tritici-resistant gene Yr10 and is named Xsdau79. Through detecting the molecular marker, whether the wheat gene Yr10 exists or not can be known, so that whether wheat varieties have the characteristics of puccinia striiformis westend.f.sp.tritici resistance or not can be determined. The molecular marker provided by the invention can accurately andquickly identify the puccinia striiformis westend.f.sp.tritici-resistant gene Yr10; therefore, the invention provides the application of a primer to the screening of the puccinia striiformis westend.f.sp.tritici-resistant wheat.

Description

technical field [0001] The invention belongs to the technical field of wheat breeding, and in particular relates to a wheat gene Yr10 molecular marker and its application in screening wheat stripe rust-resistant wheat. Background technique [0002] Wheat (Triticum aestivum L.) is the second largest grain crop in my country, with an annual planting area of ​​more than 24 million hectares and a total output of about 120 million tons, accounting for 20% of the grain crop output. Therefore, wheat production plays an important role in my country's food security and economic development. [0003] Wheat stripe rust is caused by Pucciniastriiformis f.sp.tritici (Pst), which mainly occurs on wheat leaves, secondly damages wheat leaf sheaths and stems, and occasionally spreads to ear glumes and awns. Wheat stripe rust likes coolness and fears heat. It maintains activity and reproduces in cool climates, mainly relying on uredia spores to spread back and forth, and completes the annual...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 苑翠玲吴佳洁闫百蔷吴竞争吕波倪飞付道林
Owner SHANDONG AGRICULTURAL UNIVERSITY
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