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Antibacterial peptide-chitosan compound as well as preparation method and application thereof

A technology of antibacterial peptide and chitosan, which is applied in antibacterial drugs, local antibacterial agents, drug combinations, etc., can solve the problems of antibacterial range and antibacterial effect that do not meet expectations

Inactive Publication Date: 2018-05-08
肽源(广州)生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its antibacterial ability is related to molecular weight and pH value, and its antibacterial range and antibacterial effect have not met expectations.

Method used

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  • Antibacterial peptide-chitosan compound as well as preparation method and application thereof
  • Antibacterial peptide-chitosan compound as well as preparation method and application thereof
  • Antibacterial peptide-chitosan compound as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] The preparation of embodiment 1 antimicrobial peptide-chitosan complex

[0066] In this embodiment, taking the antibacterial peptide D-peptide A and chitosan as an example, the preparation steps are as follows:

[0067] (1) Accurately weigh 100mg chitosan (molecular weight 3.5-10KD, purchased from Zhejiang Golden Shell Pharmaceutical Co., Ltd.), dissolve it in 10mL PBS buffer solution (pH 6),

[0068] (2) Accurately weigh 100mg D-peptide A, dissolve in 10mL PBS buffer (pH 6),

[0069] (3) Accurately weigh 80mg 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC-HCl) and 220mg N-hydroxysuccinimide (NHS), add to In the PBS solution of D-peptide A, stir for ten minutes at 4°C in the dark to activate the carboxyl group,

[0070] (4) The chitosan solution prepared in step (1) was added dropwise in the PBS solution of D-peptide A, stirred overnight at 4°C in the dark,

[0071] (5) transfer the solution of step (4) to a dialysis bag with a molecular weight cut-...

Embodiment 2

[0075] Embodiment 2 antimicrobial peptide-chitosan infrared spectrum detection

[0076]Take appropriate amount of antimicrobial peptide-chitosan complex prepared in Example 1, chitosan and antimicrobial peptide lyophilized powder mixed with KBr powder (1: 10~20, w / w), grind and mix and put into a compression molder to press Form into flakes and place in a dryer to dry. Put a blank KBr sheet in the Fourier transform infrared spectrometer to collect the background, then replace it with the KBr sheet of antimicrobial peptide-chitosan complex (PT-COS), chitosan (COS) and antimicrobial peptide (PT) samples, and scan to get the original spectrum After that, follow-up baseline correction, curve smoothing and water vapor compensation etc. are carried out.

[0077] see results figure 1 . The results show: figure 1 Chitosan (COS), 3405cm -1 The strong broad peaks on the left and right are multiple absorption peaks formed by overlapping the stretching vibration peak of -NH and the s...

Embodiment 3

[0078] The preparation of embodiment 3 antimicrobial peptide-sulfonated chitosan-cell growth factor nanoparticles

[0079] In this embodiment, taking the antibacterial peptide D-peptide A and sulfonated chitosan as an example, the preparation steps are as follows:

[0080] (1) Accurately weigh 100 mg of sulfonated chitosan (molecular weight is 5.2KD, prepared by the Medical Biotechnology Research and Development Center of Jinan University in Guangzhou according to standard chemical methods, the synthesis method can be found in Hong-weiWang, LinYuan, Tie-liangZhao, et al .Altered enzymatic activity of lysozymes bound to variouslysulfated chitosans[J].Chinese Journal of Polymer Science, 2012,30(6):893-899.), dissolved in 10mL PBS buffer (pH 6),

[0081] (2) Accurately weigh 100mg D-peptide A, dissolve in 10mL PBS buffer (pH 6),

[0082] (3) Accurately weigh 80mg EDC-HCl and 220mg NHS, add to the PBS solution of D-peptide A, stir for ten minutes at 4°C in the dark to activate th...

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Abstract

The invention discloses a chitosan antibacterial material modified by an antibacterial peptide as shown in SEQ ID Nos. 1 to 4. Specifically, the invention provides an antibacterial peptide-chitosan compound. Carboxyl on the antibacterial peptide and amino on the chitosan are coupled through an acylation reaction. The antibacterial peptide modified-chitosan material is obvious in antibacterial effect, and can obviously inhibit staphylococcus aureus, white staphylococcus, propionibacterium acnes or escherichia coli. Besides, the invention discloses a nanoparticle prepared from a cell growth factor loaded by the material and a preparation method of the nanoparticle. A cosmetic or a skin care product prepared by compounding the nanoparticle used as a raw material with other auxiliary materialshas the effects of suppressing acne growth and repairing the acne-damaged skin.

Description

technical field [0001] The invention belongs to the field of cosmetics and skin care products. The invention discloses a chitosan compound modified by an antimicrobial peptide, in particular to a chitosan compound modified by an antimicrobial peptide shown in SEQ ID Nos.1-4, and at the same time discloses a cell loaded with the material Nanoparticles made of growth factors and methods for their preparation. The nanoparticles can be further prepared into forms such as aqueous solution, emulsion, gel, liposome and the like. Background technique [0002] In the 1980s, a class of peptides with antibacterial activity discovered by Swedish scientists was called "antimicrobial peptides", meaning "antimicrobial peptides". Antimicrobial peptides are polypeptides synthesized in ribosomes encoded by genes. Different types of antimicrobial peptides usually have common characteristics: short peptides (30-60 amino acids), strong cationic properties (isoelectric point range: 8.9-10.7), t...

Claims

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Application Information

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IPC IPC(8): A61K8/73A61K8/64A61K8/02A61Q19/00A61Q17/00A61P17/10A61P17/02A61P31/02A61P31/04
CPCA61K8/0245A61K8/64A61K8/736A61K2800/5922A61Q17/005A61Q19/00Y02A50/30
Inventor 黄亚东项琪肖巧学贝煜
Owner 肽源(广州)生物科技有限公司
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