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Application of skin color-associated SNP marker in genotyping of black-bone chickens

A genotyping and labeling technology, applied in recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problems of not being suitable for a large number of sample detection, complicated SSCP operation, high detection cost, etc., and achieve fast and accurate individual The effect of screening, low cost and simple operation process

Active Publication Date: 2018-05-04
LESHAN NORMAL UNIV
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AI Technical Summary

Problems solved by technology

However, these methods have certain disadvantages. Among them, SSCP is complex and time-consuming, and is not suitable for the detection of a large number of samples.
The cost of sequencing and TaqMan probe genotyping is too high, and RFLP is not suitable for a large number of samples

Method used

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  • Application of skin color-associated SNP marker in genotyping of black-bone chickens
  • Application of skin color-associated SNP marker in genotyping of black-bone chickens
  • Application of skin color-associated SNP marker in genotyping of black-bone chickens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] Materials and Methods

[0034] Experimental animals and sample collection:

[0035] A total of 188 healthy adult Muchuan black-bone chickens were selected in this experiment, and the experimental subjects were all under the unified feeding and management conditions of Muchuan County Heifenghuang Silky Chicken Industry Co., Ltd. Blood collection: Use a disposable blood collection needle to collect blood through the wing vein into a vacuum blood collection tube containing sodium citrate anticoagulant, and mix well. At the same time, the skin color (black, light black, white) of silky chickens was recorded.

[0036] equipment:

[0037] Low-temperature high-speed centrifuge, ultra-micro spectrophotometer, adjustable micro pipette, gel imaging system, electronic balance, high-pressure sterilizer, constant temperature water bath, electrophoresis apparatus, gradient PCR apparatus.

[0038] Genomic DNA Extraction

[0039] 1. Put 20ul anticoagulated whole blood into a 1.5ml ...

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Abstract

The invention provides an application of a skin color-associated SNP marker in genotyping of black-bone chickens. The SNP marker is located at 2228 bp upstream of a transcription initiation site in apromoter region of a TYR gene, and a base with polymorphism is adenine A or thymine T. The SNP genotype detection primer has high specificity, single detection band and accurate typing results. In combination with a provided detection method, the whole operation process is simpler and more efficient, and the cost is lower. Rapid and accurate individual screening is achieved, and a basis for breeding development and protection of black-bone chicken varieties is provided.

Description

technical field [0001] The invention belongs to the field of molecular biology detection, and in particular relates to the application of a color-related SNP marker in silky chicken genotyping. Background technique [0002] Chicken skin color is a very important breed characteristic as a quality trait. Chicken skin color has an important economic use due to different market consumption needs. The skin color of poultry is mainly divided into three types: white, yellow and black, and the black skin is represented by silky chicken. [0003] Black-bone chicken has special nourishing and medicinal value, and its most notable feature is that it contains a large amount of melanin in the body. As an important active substance, black-bone chicken melanin has the functions of scavenging free radicals, anti-oxidation, delaying aging and improving body immunity. Studies have shown that black-bone chicken melanin can enrich trace elements such as Cu, Mn, Fe, Co and Ni, and the above e...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 喻世刚王钢廖娟
Owner LESHAN NORMAL UNIV
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