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Method for preparing antioxidant pine pollen peptide by utilizing pine pollen meal

A technology of pine pollen and anti-oxidation, applied in the biological field, can solve problems such as no reports of antioxidant peptides, and achieve the effects of reasonable amino acid ratio, low reaction temperature and high safety.

Active Publication Date: 2018-05-04
YANTAI NEW ERA HEALTH IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the domestic research on the preparation of antioxidant peptides by hydrolyzing pine pollen meal with protease

Method used

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  • Method for preparing antioxidant pine pollen peptide by utilizing pine pollen meal
  • Method for preparing antioxidant pine pollen peptide by utilizing pine pollen meal
  • Method for preparing antioxidant pine pollen peptide by utilizing pine pollen meal

Examples

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Embodiment 1

[0028] A method for preparing antioxidant pine pollen peptides by using pine pollen meal, the steps are as follows:

[0029] (1) Pulverize the pine pollen meal with a pulverizer, dissolve 50g pine pollen meal in 750mL, 80wt% ethanol, stir and extract in a water bath at 60°C for 2h, centrifuge at 4000r / min for 15min, and deposit the centrifuged precipitate in the natural After standing for 20 hours under ventilated conditions, add water to be mixed with 6.5% pine pollen meal solution by mass percent;

[0030] (2) Adjust the pH of the pine pollen meal solution to 11.5 with sodium hydroxide solution, stir and extract in a water bath at 60°C for 1 hour, centrifuge at 4000r / min for 15 minutes, collect the supernatant, and adjust the supernatant with hydrochloric acid solution pH to 4.8, centrifuge at 4000r / min for 15min, collect the precipitate, add water to prepare a 2.7wt% pine pollen protein dispersion, and adjust the pH to 7.5 to obtain a pine pollen protein isolate solution; ...

Embodiment 2

[0046] A method for preparing antioxidant pine pollen peptides by using pine pollen meal, the steps are as follows:

[0047] (1) Pulverize the pine pollen meal with a pulverizer, dissolve 30g pine pollen meal in 300mL, 80wt% ethanol, stir and extract in a water bath at 60°C for 2h, centrifuge at a speed of 4000r / min for 15min, and place the precipitate collected by centrifugation in a natural After standing for 24 hours under ventilated conditions, add water to prepare a 6.5% pine pollen solution by mass;

[0048] (2) Adjust the pH of the pine pollen meal solution to 11.5 with sodium hydroxide solution, stir and extract in a water bath at 60° C. for 1 hour, centrifuge at 4000 r / min for 15 minutes, collect the supernatant, and adjust the pH to 5.0 with hydrochloric acid solution. Centrifuge at 4000r / min for 15min, collect the precipitate, add water to prepare a 2.7wt% pine pollen protein dispersion, and adjust the pH to 7.5 to obtain a pine pollen protein isolate solution;

[...

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Abstract

The invention belongs to the technical field of biology and in particular relates to a method for preparing an antioxidant pine pollen peptide by utilizing pine pollen meal. According to the method disclosed by the invention, lots of pine pollen meal residues and wastes are reutilized in the process of preparing aqueous extract of pine pollen, lots of high-quality protein resources in the residuesare fully utilized, and the environment stress is relieved. The prepared pine pollen peptide with antioxidant activity has the characteristics of being high in safety, reasonable in amino acid ratioand the like, and has wide market prospects in the field of foods. The provided preparation method is simple in process and low in reaction temperature, the oxidation resistance of pine pollen proteins can be improved, and the pine pollen peptide prepared under the optimal process condition has a superoxide anion inhibition rate of 18.7%, reaches a DPPH (1,1-diphenyl-2-picryl-hydrazyl) removal rate of 76.8%, reaches a hydroxyl radical inhibition rate of 94.6%, and provides high-quality natural antioxidants for industries of foods, medicines and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing anti-oxidative pine pollen peptides from pine pollen meal. Background technique [0002] The aging mechanism of human body and the mechanism of disease generation have long been major scientific issues that scholars at home and abroad are committed to solving. In recent years, with the introduction of Harman's free radical theory, people's understanding of free radicals produced by oxidation in the human body has gradually deepened, and it has been found that human aging and the occurrence of various diseases are related to the excessive production of free radicals. Exploring a safe and efficient way to eliminate free radicals has gradually become a research hotspot among current scholars. Antioxidants are a class of substances that can eliminate free radicals that have developed rapidly in recent years, including chemically synthesized antioxidant...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/14
CPCC07K1/14C07K1/34C07K1/36C12P21/06
Inventor 尹利端王桐鞠东徐宏楠杨长军仲米存孙海军
Owner YANTAI NEW ERA HEALTH IND
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