Method for extracting synephrine from ripe navel orange peels

A technology of navel orange and peel, applied in the field of natural plant extraction

Active Publication Date: 2018-04-24
HEZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, most studies on synephrine are mainly extracted from Citrus aurantium, and there are few reports on extracting from mature pericarp.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting synephrine from ripe navel orange peel, comprising the following steps:

[0023] (1) Pulverization: Take the dried navel orange peel and pulverize it into particles with a particle size of 0.03 mm to 0.3 mm to obtain navel orange peel powder.

[0024] (2) The navel orange peel powder and distilled water are mixed according to the ratio of 1g: 10mL according to the weight to volume ratio, then add 0.6wt% pectin-cellulose compound enzyme of the weight of the navel orange peel powder weight, and enzymolyze it in a water bath at 55°C for 1h, Obtain the enzymatic hydrolysis mixture. Wherein, in the pectin-cellulose compound enzyme, the mass ratio of pectin and cellulase is 6:1, the enzyme activity of pectinase is 50000U / g, and the enzyme activity of cellulase is 60000U / g.

[0025] (3) The enzymolysis mixture is placed in an autoclave for ultra-high pressure deactivation treatment, the pressure is 300MPa, the time for deactivation treatment is 15min, ...

Embodiment 2

[0029] A method for extracting synephrine from ripe navel orange peel, comprising the following steps:

[0030] (1) Pulverization: Take the dried navel orange peel and pulverize it into particles with a particle size of 0.03 mm to 0.3 mm to obtain navel orange peel powder.

[0031] (2) The navel orange peel powder and distilled water are mixed according to the ratio of 1g: 15mL according to the weight volume ratio, then add 0.8wt% pectin-cellulose compound enzyme of the weight of the navel orange peel powder weight, and enzymolyze it in a water bath at 50°C for 2h, Obtain the enzymatic hydrolysis mixture. Wherein, in the pectin-cellulose compound enzyme, the mass ratio of pectin and cellulase is 5:1, the enzyme activity of pectinase is 50000U / g, and the enzyme activity of cellulase is 60000U / g.

[0032] (3) The enzymolysis mixture is placed in an autoclave for ultra-high pressure deactivation treatment, the pressure is 200MPa, the time for deactivation treatment is 25min, and...

Embodiment 3

[0036] A method for extracting synephrine from ripe navel orange peel, comprising the following steps:

[0037] (1) Pulverization: Take the dried navel orange peel and pulverize it into particles with a particle size of 0.03 mm to 0.3 mm to obtain navel orange peel powder.

[0038] (2) The navel orange peel powder and distilled water are mixed according to the ratio of 1g: 12mL according to the weight volume ratio, and then 0.7wt% pectin-cellulose compound enzyme of the weight of the navel orange peel powder weight is added, and the enzyme is hydrolyzed in a water bath at 60°C for 1h, Obtain the enzymatic hydrolysis mixture. Wherein, in the pectin-cellulose compound enzyme, the mass ratio of pectin and cellulase is 7:1, the enzyme activity of pectinase is 50000U / g, and the enzyme activity of cellulase is 60000U / g.

[0039](3) The enzymolysis mixture is placed in an autoclave for ultra-high pressure deactivation treatment, the pressure is 250MPa, the time for deactivation trea...

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Abstract

The invention discloses a method for extracting synephrine from ripe navel orange peels. The method comprises the steps as follows: (1) dried navel orange peels are crushed and then mixed with distilled water, complex enzyme is added for enzymolysis, and an enzymolysis mixture is obtained; (2) the enzymolysis mixture is subjected to ultrahigh-pressure enzyme deactivation treatment, the pH is adjusted to 5.0-6.0, subcritical extraction is performed under nitrogen protection, centrifugation is performed after extraction, and a liquid supernatant is collected; (3) the liquid supernatant obtainedin the step (2) is subjected to ultrafiltration separation with an ultrafiltration membrane with the molecular interception being 5,000 u-10,000 u, and a concentrated solution is collected; (4) the concentrated solution obtained in the step (3) is mixed and stirred with ethanol, the obtained mixed solution is subjected to reduced pressure suction filtration and freeze drying, and synephrine is obtained. The method has the advantages of being high in extraction rate and extraction efficiency, mild in treatment condition, environmentally friendly and the like.

Description

technical field [0001] The invention belongs to the technical field of natural plant extraction, in particular to a method for extracting synephrine from ripe navel orange peel. Background technique [0002] Navel oranges are abundant in Fuchuan County, Hezhou City, Guangxi, and their peels are the main by-products in the process of eating and processing. According to the survey, there is less comprehensive utilization of tribute orange and navel orange peels in this area. At present, except for a small amount of citrus peels used for processing tangerine peels, there is basically no other use. Most of them are discarded as waste, which not only wastes precious resources, but also seriously pollute the environment. In fact, citrus peels contain many active ingredients such as synephrine and hesperidin, so they are raw materials with potential industrial production value. Synephrine is an alkaloid that constricts blood vessels, raises blood pressure, and strongly dilates tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C213/10C07C215/60
CPCC07C213/10C07C215/60
Inventor 蔡文段振华帅良
Owner HEZHOU UNIV
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