Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

D-dimer detection kit and use method thereof

A detection kit and dimer technology, applied in the direction of measurement device, analysis by chemical reaction of materials, instruments, etc., can solve the problems of cumbersome operation and narrow detection range, simplify the operation steps, meet the requirements of rapid diagnosis, The effect of improving the detection speed

Active Publication Date: 2018-04-20
NANTONG EGENS BIOTECH
View PDF7 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is that the existing D-dimer detection kit uses plasma or serum as a sample, which has the defects of cumbersome operation and narrow detection range, thereby providing a method that can directly use whole blood as a sample and detect A wide range of D-dimer detection kits; further, the present invention also provides a method for using the detection kit

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • D-dimer detection kit and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The preparation of embodiment 1 enzyme conjugate

[0055] 1. Weigh 3mg of Traut's reagent, and prepare a solution with a concentration of 1.376mg / mL with 100mmol / L triethanolamine buffer (pH=8.5±0.05); weigh 0.5mg of D-dimer labeled antibody, and use 100mmol / L triethanolamine buffer solution (pH=8.5 ± 0.05) was prepared into a solution with a concentration of 2mg / mL, and to it was added a concentration of 1.376mg / mL of Traut's solution, D-dimer labeled antibody and Traut The molar ratio of 's reagent is 1:15, mix immediately, and react at room temperature for 15 minutes. Add the glycine solution of 1mmol / L, the amount of substance of glycine is 10 of the amount of 4-(N-maleimidomethyl) cyclohexane-1-carboxylic acid sulfosuccinimide ester sodium salt substance times, mix immediately, and react at room temperature for 10 minutes. Desalted and replaced with 100 mmol / L triethanolamine cross-linking buffer (pH=7.3±0.05).

[0056] 2. Weigh 3 mg of 4-(N-maleimidomethyl) cyc...

Embodiment 2

[0060] The preparation of embodiment 2 enzyme conjugates

[0061] 1. Weigh 3mg of Traut's reagent, and prepare a solution with a concentration of 1.3mg / mL with 100mmol / L triethanolamine buffer solution (pH=8.5±0.05); weigh 0.5mg of D-dimer labeled antibody, and use 100mmol / L triethanolamine buffer solution (pH=8.5 ± 0.05) was prepared into a solution with a concentration of 5mg / mL, and to it was added a concentration of 1.3mg / mL Traut's solution, D-dimer labeled antibody and Traut The molar ratio of 's reagent is 1:10, mix immediately, and react at room temperature for 12 minutes. Add the glycine solution of 1mmol / L, the amount of substance of glycine is 20% of the amount of 4-(N-maleimidomethyl) cyclohexane-1-carboxylic acid sulfosuccinimide ester sodium salt substance times, mix immediately, and react at room temperature for 12 minutes. Desalted and replaced with 100 mmol / L triethanolamine cross-linking buffer (pH=7.3±0.05).

[0062] 2. Weigh 3 mg of 4-(N-maleimidomethyl)...

Embodiment 3

[0066] The preparation of embodiment 3 enzyme conjugates

[0067]1. Weigh 3mg of Traut's reagent, and prepare a solution with a concentration of 1.5mg / mL with 100mmol / L triethanolamine buffer solution (pH=8.5±0.05); weigh 0.5mg of D-dimer labeled antibody, and use 100mmol / L triethanolamine buffer solution (pH=8.5±0.05) was prepared into a solution with a concentration of 2.5mg / mL, and to which was added a concentration of 1.5mg / mL Traut's solution, D-dimer labeled antibody and The molar ratio of Traut's reagent was 1:15, mixed immediately, and reacted at room temperature for 18 minutes. Add the glycine solution of 1mmol / L, the amount of substance of glycine is 15% of the amount of 4-(N-maleimidomethyl) cyclohexane-1-carboxylic acid sulfosuccinimide ester sodium salt substance times, mix immediately, and react at room temperature for 10 minutes. Desalted and replaced with 100 mmol / L triethanolamine cross-linking buffer (pH=7.3±0.05).

[0068] 2. Weigh 3 mg of 4-(N-maleimidom...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Concentrationaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of in vitro diagnosis kits, and concretely relates to a D-dimer detection kit and a use method thereof. The D-dimer detection kit comprises a calibration product, areagent R1, an enzyme conjugate working solution R2, a magnetic bead conjugate working solution M, a cleaning solution and a chemiluminescent substrate, and the reagent R1 contains an imidazole component, so blood cells in the whole blood can be quickly eliminated to effectively avoid the possibility of blood cells swallowing magnetic beads. The kit can directly use fingertip whole blood or anticoagulant venous whole blood as a sample to be tested, and can directly detect the whole blood sample without preprocessing the whole blood sample, so the detection speed is greatly improved, the operation steps are simplified, the application range of the kit is enlarged, and large-scale promotion and application are easy.

Description

technical field [0001] The invention belongs to the field of in vitro diagnostic kits, and in particular relates to a D-dimer detection kit and a use method thereof. Background technique [0002] The fibrinolytic system is the most important anticoagulant system in the human body. It plays an important role in maintaining the normal permeability of the blood vessel wall, maintaining blood flow and tissue repair. The system consists of 4 main parts: plasminogen , plasminogen activator, plasmin and plasmin inhibitor. When a fibrin clot is formed, in the presence of plasminogen activators, plasminogen is activated and converted into plasmin, the process of fibrinolysis begins, and plasmin degrades the fibrin clot to form various soluble fragments, Fibrin degradation products (FDP) are formed. Among them, D-dimer is the smallest fragment in the degradation products, which is a specific degradation product of cross-linked fibrin, with a molecular weight of about 62ku and a half...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/543G01N33/535G01N33/531G01N21/76
CPCG01N21/76G01N33/531G01N33/535G01N33/54326
Inventor 王保君汤双双欧卫军徐艳褚晖
Owner NANTONG EGENS BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com