Method for embedding and producing probiotic powder from arctium lappa root dry powder and milk powder
A technology of probiotics, root dry powder, applied in the directions of bacteria, application, lactobacillus used in food preparation, etc., to achieve the effect of high activity
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specific Embodiment 1
[0026] (1) Configuration of MRS solid medium: 10g / L peptone, 10g / L beef extract, 5g / L yeast powder, 20g / L glucose, 5g / L anhydrous sodium acetate, 1ml / L Tween-80, 2g / L L diamine citrate, 2g / L dipotassium hydrogen phosphate, 0.58g / L magnesium sulfate, 0.25g / L manganese sulfate, dissolved in distilled water, adjust pH 6.6-6.8, then add 2% agar, constant volume; high pressure 1 ×10 5 Pa sterilization 20min;
[0027] (2) Configuration of MRS liquid medium: 10g / L peptone, 10g / L beef extract, 5g / L yeast powder, 20g / L glucose, 5g / L anhydrous sodium acetate, 1ml / L Tween-80, 2g / L L diamine citrate, 2g / L dipotassium hydrogen phosphate, 0.58g / L magnesium sulfate, 0.25g / L manganese sulfate, dissolved in distilled water, adjust pH 6.6-6.8, and constant volume; high pressure 1×10 5 Pa sterilization 20min;
[0028] (3) Preparation of artificial gastric juice: Dissolve 0.2g NaCl and 0.35g pepsin in appropriate amount of distilled water, adjust the pH value to 2.0 with HCl, and set the volum...
specific Embodiment 2
[0040] (1) Configuration of MRS solid medium: 10g / L peptone, 10g / L beef extract, 5g / L yeast powder, 20g / L glucose, 5g / L anhydrous sodium acetate, 1ml / L Tween-80, 2g / L L diamine citrate, 2g / L dipotassium hydrogen phosphate, 0.58g / L magnesium sulfate, 0.25g / L manganese sulfate, dissolved in distilled water, adjust pH 6.6-6.8, then add 2% agar, constant volume; high pressure 1 ×10 5 Pa sterilization 20min;
[0041] (2) Configuration of MRS liquid medium: 10g / L peptone, 10g / L beef extract, 5g / L yeast powder, 20g / L glucose, 5g / L anhydrous sodium acetate, 1ml / L Tween-80, 2g / L L diamine citrate, 2g / L dipotassium hydrogen phosphate, 0.58g / L magnesium sulfate, 0.25g / L manganese sulfate, dissolved in distilled water, adjust pH 6.6-6.8, and constant volume; high pressure 1×10 5 Pa sterilization 20min;
[0042] (3) Preparation of artificial gastric juice: Dissolve 0.2g NaCl and 0.35g pepsin in appropriate amount of distilled water, adjust the pH value to 2.0 with HCl, and set the volum...
specific Embodiment 3
[0054] (1) Configuration of MRS solid medium: 10g / L peptone, 10g / L beef extract, 5g / L yeast powder, 20g / L glucose, 5g / L anhydrous sodium acetate, 1ml / L Tween-80, 2g / L L diamine citrate, 2g / L dipotassium hydrogen phosphate, 0.58g / L magnesium sulfate, 0.25g / L manganese sulfate, dissolved in distilled water, adjust pH 6.6-6.8, then add 2% agar, constant volume; high pressure 1 ×10 5 Pa sterilization 20min;
[0055] (2) Configuration of MRS liquid medium: 10g / L peptone, 10g / L beef extract, 5g / L yeast powder, 20g / L glucose, 5g / L anhydrous sodium acetate, 1ml / L Tween-80, 2g / L L diamine citrate, 2g / L dipotassium hydrogen phosphate, 0.58g / L magnesium sulfate, 0.25g / L manganese sulfate, dissolved in distilled water, adjust pH 6.6-6.8, and constant volume; high pressure 1×10 5 Pa sterilization 20min;
[0056] (3) Preparation of artificial gastric juice: Dissolve 0.2g NaCl and 0.35g pepsin in appropriate amount of distilled water, adjust the pH value to 2.0 with HCl, and set the volum...
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