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Light yellow golden mushroom JK05 bacterial strain and application thereof

A technology of light yellow golden needles and strains, applied in the fields of application, fungi, mushroom cultivation, etc., can solve the problems of incapable of industrial vertical bottle cultivation, dark color, and poor quality, and achieve resistance to Trichoderma and Pseudomonas aeruginosa The effect of strong ability, straight stipe and short cultivation period

Active Publication Date: 2018-04-06
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] The object of the present invention is to provide a light yellow Flammulina velutipes JK05 bacterial strain, spores, mycelium and fruiting bodies obtained by said bacterial strain, the application of said bacterial strain in hybrid breeding and the application of said fruiting bodies in food processing; The invention also provides a method for cultivating the strain and a culture medium for cultivating the fruiting body, so as to solve the existing problems in the current production of Flammulina velutipes, such as dark color, poor quality, serious "spit water" during the cultivating process, and cannot be planted. Problems such as vertical bottle cultivation

Method used

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  • Light yellow golden mushroom JK05 bacterial strain and application thereof
  • Light yellow golden mushroom JK05 bacterial strain and application thereof

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Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1 hybridization obtains Flammulina velutipes JK05

[0037] The Flammulina velutipes strain JK05 was obtained by cross breeding.

[0038] Specific steps are as follows:

[0039] 1) Select the parent strains: select the strain Taichangbaiheza 19 as the parent, and the fruiting bodies of the two parents can be purchased in the market.

[0040] 2) Preparation of mother seed medium, liquid seed medium and cultivation medium: the formulations of each medium have been given, and will not be repeated here.

[0041] 3) Obtain 200 heterozygotes (F1 generation) by conventional cross-breeding methods.

[0042] 4) Cultivation and screening: The obtained heterozygotes were subjected to cultivation tests according to conventional methods, and a target strain Jinza 9 was obtained from 200 heterozygotes (F1 generation) through systematic screening. Strong resistance, early fruiting, high yield, light yellow color, thick stipe, not easy to open umbrellas.

[0043] 5) Colle...

Embodiment 2

[0045] Example 2 Strain Antagonism Experiment and ISSR Authenticity Analysis

[0046] The light yellow Flammulina velutipes JK05 of the present invention and two parent strains Taichangbai and Za19 are test materials, and the culture medium used is PDA culture medium.

[0047] Flammulina velutipes JK05 and Taichangbai, JK05 and Za 19 were inoculated and cultured on the plate medium respectively, the inoculation points were 4 cm apart, and cultured at 25°C for 15 days. The results showed that the antagonistic lines of JK05 and Taichangbai, JK05 and Za19 were obvious at the junction of confrontation culture colonies, which indicated that JK05 was a different Flammulina velutipes strain from Taichangbai and Za19.

[0048] Authenticity Analysis of Flammulina velutipes JK05 strain by ISSR method

[0049] In order to prove the differences between the JK05 strain and the parental species Taichangbai and Za19, as well as the existing white Flammulina velutipes varieties F21 and Rijin...

Embodiment 3

[0053] Embodiment 3 disease resistance test

[0054] Trichodermaviride and pathogenic Pseudomonas aeruginosa (P.Aeruginosa) are the main diseases in the cultivation of Flammulina velutipes. The pathogenic bacteria Trichoderma viride and Pseudomonas aeruginosa isolated from Flammulina velutipes and Pseudomonas aeruginosa isolated and preserved in our unit were used as indicators to carry out confrontation culture. The disease resistance can be judged by observing the growth of the colony and the width of the antagonistic band. The narrower the antagonistic band, the stronger the disease resistance.

[0055] Activation of strains: Activation culture of Flammulina velutipes strain and Trichoderma virides strain in PDA medium at 25°C respectively, and the dish is ready for use after the dish is full. Before being used as bacteria species, bacteria cakes were prepared with a puncher with a diameter of 0.5 cm. Pseudomonas aeruginosa was activated in PDA medium at 25°C for 2 days, ...

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Abstract

The invention relates to a light yellow golden mushroom JK05 bacterial strain, a preservation number is CGMCC No.13879, and the invention also provides spore, mycelium and sporophore obtained by the bacterial strain, an application of the bacterial strain in cross breeding, and an application of the sporophore in food processing. The invention also provides a culture method of the bacterial strainand a cultivation medium used for culturing the sporophore, which can solve the problems that during yellow golden mushroom production, color is deep, condition is poor, guttation during a culture process is severe, and the golden mushroom is not suitable for bottle cultivation in a factory.

Description

technical field [0001] The invention belongs to the technical field of edible fungi, and in particular relates to the light yellow Flammulina velutipes JK05 strain and application thereof. Background technique [0002] The scientific name of Flammulina velutiper (Flammulinavelutiper (Fr.) Sing) is rich in a variety of nutrients. It is not only a delicious food, but also a good health food. It plays an important role in daily vegetable consumption. Flammulina velutipes varieties are divided into yellow Flammulina velutipes varieties and white Flammulina velutipes varieties according to fruiting body color. Yellow Flammulina velutipes varieties are generally cultivated by farmers. Compared with white Flammulina velutipes varieties, they have the characteristics of strong resistance, fast growth, thick stipe, high temperature resistance, good flavor and taste, such as Za 19, Su 6, etc.; white Flammulina velutipes varieties are mainly industrialized Cultivation, white color, g...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00A01G18/40A01G18/00A01G18/20C12R1/645
CPCC12N1/14C12N3/00C12N1/145C12R2001/645
Inventor 李书生张根伟刘昆昂刘振国马宏尹淑丽付艳菊
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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