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Bioactive polypeptide VPITPTLNR as well as preparation method and application thereof

A technology of biologically active polypeptides and derivatives, applied in the field of proteins, can solve problems such as human body risks, and achieve the effects of reducing body aging, improving quality, and improving anti-aging activity.

Active Publication Date: 2018-04-06
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Some early synthetic antioxidants such as butyl hydroxyanisole (BHA) and 2,6-di-tert-butyl-4-methylphenol (BHT) were used in food as lipid antioxidants, but these artificial Synthetic additives are potentially dangerous to the human body

Method used

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  • Bioactive polypeptide VPITPTLNR as well as preparation method and application thereof
  • Bioactive polypeptide VPITPTLNR as well as preparation method and application thereof
  • Bioactive polypeptide VPITPTLNR as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The artificial synthesis of embodiment 1 active peptide VPITPTLNR

[0036] 1. Synthesis of Bioactive Peptides

[0037] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak with 50ml of dichloromethane (DCM).

[0038] 2. After 2 hours, wash the resin with nitrogen-dimethylformamide (DMF) that is 3 times the volume of the resin, and then drain it. Repeat this four times, and drain the resin for use.

[0039] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0040] 4. Take a small amount of resin and test it with ninhydrin (Ninhydrin Nine Wells) method (two drops each of test A and test B, react at 100°C for 1 min), if the resin has color, it means that the deprotection is successful.

[0041] 5. Weigh an...

Embodiment 2

[0077] Antioxidant activity experiment of embodiment 2 bioactive peptides

[0078] 1. Determination of in vitro antioxidant activity of biologically active peptide VPITPTLNR by [DPPH·] method

[0079] 1. Experimental reagents and instruments:

[0080] Reagent: 1,1-diphenyl-2-trinitrophenylhydrazine (1,1-Diphenyl-2-picrylhydrazyl[DPPH·]), produced by Wako Company of Japan; Methanol, provided by Shanghai Sinopharm Company; obtained in Example 1 Milk-derived bioactive polypeptide VPITPTLNR.

[0081] Main instruments: Sunrise microplate reader, product of Tecan Company of Austria; 96-well cell culture plate, manufactured by Millipore Company of the United States; analytical balance, product of Meitelei-tolido Company.

[0082] 2. Experimental method:

[0083] (1) 1mmol / L [DPPH·]methanol solution

[0084] Weigh 0.349 mg [DPPH·] with an analytical balance and dissolve it in 1 mL of methanol solution to prepare a 1 mmol / L [DPPH·] methanol solution, store it in the dark on tin foi...

Embodiment 3

[0118] Anti-aging activity experiment of embodiment 3 bioactive peptides

[0119] 1. The experiment of biologically active polypeptide VPITPTLNR improving the viability of Drosophila

[0120] 1. Experimental reagents and instruments:

[0121] Reagent: Oregon K wild-type Drosophila melanogaster, Genetics Laboratory, College of Agriculture, Shanghai Jiao Tong University; agar powder, Sinopharm Chemical Reagent Co., Ltd.; milk-derived biologically active polypeptide VPITPTLNR obtained in Example 1.

[0122] Equipment: CM-230 Mole ultra-clean water, Shanghai Mole Scientific Instrument Co., Ltd.; G136T Zealway intelligent high-temperature sterilization pot, Xiamen Zhiwei Instrument Technology Co., Ltd.; BJ-CD SERIES biological incubator, Shanghai Boxun Industrial Company; GRX-9073 hot air disinfection box, Shanghai Yiheng Technology Co., Ltd.

[0123] 2. Experimental method:

[0124] Take Drosophila as the experimental model: collect newly eclosioned Drosophila adults within 8 h...

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Abstract

The invention relates to the field of proteins, in particular to a bioactive polypeptide VPITPTLNR as well as a preparation method and application thereof. An amino acid sequence of the bioactive polypeptide VPITPTLNR is Val-Pro-Ile-Thr-Pro-Thr-Leu-Asn-Arg. According to an in-vitro anti-oxidation test and an in-vivo anti-ageing test, the polypeptide VPITPTLNR is verified to have a relatively goodanti-oxidation bioactivity and relatively good anti-ageing activity, so that the bioactive polypeptide VPITPTLNR has relatively good anti-oxidization activity, can eliminate free radicals in an organisms, improve life quality, can improve activity of an in-vivo anti-peroxidase system and strengthen a function that the organism resists exogenous stimulus, reduce the ageing, senility and illness probability of the organism, and therefore, the bioactive polypeptide VPIIPTLNRE is of great importance in developing food, healthcare products and drugs with anti-oxidation functions and anti-ageing functions.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a biologically active polypeptide VPITPTLNR and its preparation method and application. Background technique [0002] During the fermentation process of milk by lactic acid bacteria, a part of protein in milk is metabolized and utilized by lactic acid bacteria, and a series of physiological and biochemical reactions occur, making the protein into polypeptides or free amino acids, which are digested and absorbed by the human body or absorbed and transported by small intestinal epithelial cells directly into the blood circulation of the human body. Among these peptides, some have special physiological functions and are called "bioactive peptides". [0003] It is especially important to find safe bioactive peptides in natural food sources. In recent years, it has been found that some food-derived polypeptides have good biological activity, such as corn short peptides, soybean peptides, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N15/12A23L33/18A61K38/17A61K8/64A61P39/06A61Q19/08
CPCA23L33/18A23V2002/00A61K8/64A61K38/00A61Q19/08C07K14/4732A23V2250/55A23V2200/302
Inventor 张少辉张伯宇林学海李云飞陈静汪超范梦珠李婉如
Owner ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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