Biological mildewproof agent and preparation method thereof

Abstract

The invention discloses a biological mildewproof agent and a preparation method thereof. The biological mildewproof agent comprises the following raw materials in terms of 100 parts of water by weight: 2 to 8 parts of antibacterial protein or a crude antibacterial protein product, 0.5 to 3 parts of a film forming agent, 0.5 to 5 parts of a plasticizer and 0.5 to 4 parts of a cross-linking agent. The preparation method comprises the following steps: adding water into the film forming agent, completely dissolving the film forming agent, then adding the antibacterial protein, the plasticizer andthe cross-linking agent, and carrying out uniform mixing so as to obtain a biological mildewproof solution. According to the invention, the crude antibacterial protein product is a fermentation brothof B.amyloliquefaciens F1 and is obtained by ultrafiltration and ammonium sulfate fractional precipitation, while the antibacterial protein is prepared from the above-mentioned crude antibacterial protein product through further separation means like dialysis, ion exchange and gel chromatography. The biological mildewproof solution obtained by adding a main antibacterial substance namely the antibacterial protein inhibits the growth of Aspergillus flavus, has characteristics like safety and high efficiency, and solves the problems of high price and complex operation for physical preservation and a problem of pesticide residue brought by chemical preservation. The biological mildewproof agent provided by the invention has the advantages of economy, high efficiency, convenient use, and greatapplication prospects in the fields of agriculture and food preservation.

Description

technical field [0001] The invention relates to the technical field of antibacterial, in particular to a preparation method of a biological antifungal agent. Background technique [0002] Aspergillus flavus is a deficient fungus, the colony grows rapidly, the surface is yellow-green, and the back is slightly reddish-brown. Bacterial growth usually appears many branched hyphae, forming mycelium. Degradative enzymes or proteins are secreted to break down complex nutrients once the mycelium is formed. Monobranched hyphae are usually not visible to the naked eye, but the thick mats of hyphae produced by conidia can be easily seen. Aflatoxins, the secondary metabolites produced by Aspergillus flavus, belong to a large category of mycotoxins and are widely found in various cereals (corn, rice), oil and oil crops (peanut oil, peanuts, nuts), fruits and vegetables, milk and In meat, it has teratogenic and carcinogenic hazards. [0003] There are physical methods, chemical method...

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Problems solved by technology

However, these two methods have great disadvantages. The equipment of physical prevention technology is expensive and the operation is cumbersome, and people are also worried that radiation will be harmful to the human body; problems such as drug residues caused by chemical antiseptic technology are becoming more and more worrying.

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