Drug paclitaxel treatment effect-related gene (CYP2C8 and GSTP1) polymorphism detection kit and drug paclitaxel treatment effect-related gene (CYP2C8 and GSTP1) polymorphism detection method
A gene polymorphism and detection kit technology, applied in the fields of biotechnology and medicine, can solve problems such as high price, and achieve the effects of simple method, high sensitivity and pollution reduction.
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[0026] 1. Sample Processing and Nucleic Acid Extraction
[0027] Use a commercial DNA extraction kit to process the sample. For specific operations, refer to the kit instruction manual. The concentration and purity of the extracted DNA need to be measured with a UV spectrophotometer. The DNA OD 260 / OD 280 The value should be between 1.8 and 2.0, and the concentration should be between 5 and 50 ng / μL. If the sample DNA quality is unqualified, it should not be used for testing. It is recommended that those whose DNA quality is lower than 5 ng / μL be re-taken for nucleic acid extraction, and those higher than 50 ng / μL Properly dilute to the specified concentration range. It is recommended to test the extracted DNA immediately, otherwise, please store it below -20°C, and the storage time should not exceed 6 months. The quality control product of the kit was thawed at room temperature before use, vortexed for 10 seconds, and centrifuged at 2000rpm for 15 seconds before use.
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