Drug paclitaxel treatment effect-related gene (CYP2C8 and GSTP1) polymorphism detection kit and drug paclitaxel treatment effect-related gene (CYP2C8 and GSTP1) polymorphism detection method

A gene polymorphism and detection kit technology, applied in the fields of biotechnology and medicine, can solve problems such as high price, and achieve the effects of simple method, high sensitivity and pollution reduction.

Inactive Publication Date: 2018-03-27
宁波美丽人生医药生物科技发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, related kits on the market includ

Method used

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  • Drug paclitaxel treatment effect-related gene (CYP2C8 and GSTP1) polymorphism detection kit and drug paclitaxel treatment effect-related gene (CYP2C8 and GSTP1) polymorphism detection method
  • Drug paclitaxel treatment effect-related gene (CYP2C8 and GSTP1) polymorphism detection kit and drug paclitaxel treatment effect-related gene (CYP2C8 and GSTP1) polymorphism detection method
  • Drug paclitaxel treatment effect-related gene (CYP2C8 and GSTP1) polymorphism detection kit and drug paclitaxel treatment effect-related gene (CYP2C8 and GSTP1) polymorphism detection method

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Embodiment 1

[0026] 1. Sample Processing and Nucleic Acid Extraction

[0027] Use a commercial DNA extraction kit to process the sample. For specific operations, refer to the kit instruction manual. The concentration and purity of the extracted DNA need to be measured with a UV spectrophotometer. The DNA OD 260 / OD 280 The value should be between 1.8 and 2.0, and the concentration should be between 5 and 50 ng / μL. If the sample DNA quality is unqualified, it should not be used for testing. It is recommended that those whose DNA quality is lower than 5 ng / μL be re-taken for nucleic acid extraction, and those higher than 50 ng / μL Properly dilute to the specified concentration range. It is recommended to test the extracted DNA immediately, otherwise, please store it below -20°C, and the storage time should not exceed 6 months. The quality control product of the kit was thawed at room temperature before use, vortexed for 10 seconds, and centrifuged at 2000rpm for 15 seconds before use.

[00...

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Abstract

The invention provides a drug paclitaxel treatment effect-related gene (CYP2C8 and GSTP1) polymorphism detection kit and a drug paclitaxel treatment effect-related gene (CYP2C8 and GSTP1) polymorphismdetection method, wherein the kit comprises a PCR buffer solution, specific ARMS detection primers and quality control primers. The present invention further provides the paclitaxel treatment effect-related gene (CYP2C8 and GSTP1) polymorphism detection method. According to the present invention, with the method, the paclitaxel treatment effect-related gene (CYP2C8 and GSTP1) polymorphism can berapidly and accurately detected; and the kit has characteristics of high sensitivity, strong specificity, simple method, accurate result and the like.

Description

Technical field: [0001] The invention relates to the fields of biotechnology and medicine, in particular to a detection kit and a detection method for the CYP2C8 (GSTP1) gene polymorphism related to the curative effect of the drug paclitaxel. Background technique: [0002] Most drug metabolism occurs in the liver. Cytochrome P450 (CYP or P450) is one of the main metabolic enzymes in the liver. CYP is a microsomal mixed-function monooxygenase that participates in the biological oxidation process of drugs, pro-carcinogens, poisons, mutagens and other exogenous substances. It has been found that CYP gene polymorphisms affect individual drug responses to drugs for major diseases such as depression, mental illness, tumors, cardiovascular and cerebrovascular diseases, gastric ulcers, pain and epilepsy. With the deepening of research, some drugs can also be adjusted according to the CYP genotype, so as to optimize the drug treatment effect and safety of carriers of different geno...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6858
CPCC12Q1/6858C12Q1/6886C12Q2600/106C12Q2600/156C12Q2535/137C12Q2563/107
Inventor 田晓丽
Owner 宁波美丽人生医药生物科技发展有限公司
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