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Alkaloid compound separated from pumpkin seeds and application thereof

A compound and composition technology, applied in the field of alkaloid compounds, can solve problems such as unclear functional components and inability to clarify the mechanism of efficacy, and achieve the effect of inhibiting prostate hyperplasia

Inactive Publication Date: 2018-03-27
徐一达
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the specific functional ingredients in pumpkin seeds that inhibit prostate hyperplasia are not clear, and the mechanism of efficacy cannot be clarified

Method used

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  • Alkaloid compound separated from pumpkin seeds and application thereof
  • Alkaloid compound separated from pumpkin seeds and application thereof
  • Alkaloid compound separated from pumpkin seeds and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] After crushing the pumpkin seeds, add 95% ethanol at a material-to-liquid ratio of 1:10 (m / v), stir and extract at 50-70°C for 3 hours, filter to get the supernatant, and repeat this 3 times. Concentrate the supernatant obtained three times under reduced pressure, and obtain the ethanol extract of pumpkin seeds after removing ethanol.

[0022] Extract the ethanol extract of pumpkin seeds with 2 times the volume of ethyl acetate, extract twice, combine the ethyl acetate extracts, and concentrate to obtain ethyl acetate extract.

[0023] Put the ethyl acetate extract on the MCI column, and then use deionized water, 30%, 60%, and 95% ethanol for gradient elution, collect the 95% ethanol eluted part, concentrate it under reduced pressure, and load it on the ODS column , followed by elution with 50%, 70%, and 95% ethanol, collected 95% ethanol eluted part, concentrated under reduced pressure, and loaded onto the ODS-AQ column, followed by 70%, 85%, and 95% ethanol Ethanol w...

Embodiment 2

[0025] The structures of the substances obtained in Example 1 were identified by HPLC, LC-MS and NMR respectively.

[0026] The HPLC detection conditions are: adopt ODSC18 chromatographic column, methanol:water=95:5 mobile phase, flow rate 0.5mL / min, detection wavelength 228nm chromatogram is as follows figure 1 Shown; Retention time is that the chromatographic peak at 8.39min place is squashine.

[0027] LC-MS detection conditions are: using ESI+ mode, voltage 1170v, the results are as follows figure 2 As shown, the compound m / z is 338.3303, and the calculated molecular formula is C 19 h 31 N0 4 ,

[0028] NMR detection conditions are: with D 3 Cl is solvent, tetramethylsilane is internal standard, Bruck 500M Z NMR measurements were carried out. , NMR detection results are shown in Table 1.

[0029] HR EI-MS gives a molecular mass of 337.3303, and the calculated molecular formula is C 19 h 31 N0 4 , 1 H and 13 C NMR data are shown in Table 1.

[0030] Table 1 o...

Embodiment 3

[0040] Using the model of prostatic hyperplasia induced by testosterone propionate in mice, the in vivo anti-prostatic hyperplasia test of squash seed was carried out. Male Kunming mice aged 6 weeks and weighing 20-25 g were selected, and the experimental animals were fed adaptively for 1 week in the early stage. To adapt to the new environment, they were randomly divided into groups, 10 in each group, which were model group, control group, treatment group 1 and treatment group 2, respectively. The mice in the model group and the treatment group were subcutaneously injected with 5 mg / kg of testosterone propionate (TP) every day, and the injection volume was 0.1 mL / 10 g of body weight, and the model was established continuously for 30 days to establish a mouse BPH model. Mice in the control group were subcutaneously injected with an equal volume of soybean oil daily. After the modeling was completed, the mice in the model group and the control group were intragastrically admini...

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Abstract

The invention discloses an alkaloid compound separated from pumpkin seeds and an application thereof, which belong to the technical field of food and health products. The compound is extracted from pumpkin seeds and used for treating prostatic hyperplasia, the compound has obvious inhibition effect for three indexes of prostate wet weight, prostate index and prostate acidic phosphatase (PAP) whileeffect dosage is 250 mg / kg, has obvious inhibition effect for three indexes while effect dosage is 500 mg / kg, and has important application potential.

Description

technical field [0001] The invention relates to an alkaloid compound isolated from pumpkin seeds and an application thereof, belonging to the technical field of food and health products. Background technique [0002] Pumpkin seeds are the mature seeds of pumpkin (Cucurbita spp), rich in unsaturated fatty acids, vitamins, bioflavonoids and minerals. Pumpkin seed oil has good preventive and therapeutic effects on the urinary system and benign prostatic hyperplasia. Gypsies in Hungary and Turks in Asia Minor generally eat pumpkin seeds, and men in these countries rarely suffer from enlarged prostate. In 2245 clinical cases of benign prostatic hyperplasia in Germany, 1-2 capsules of pumpkin seed extract were taken every day for 12 weeks. The results showed that 41.6% of the cases showed that the adverse symptoms of the prostate gland were reduced, and 46% of the patients’ quality of life was improved. The patients reported no intolerable problems. Side effects, it can be seen ...

Claims

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Application Information

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IPC IPC(8): C07C271/34C07C269/08A23L33/105A61K31/27A61P13/08
CPCA23L33/105A23V2002/00C07C271/34A23V2200/30
Inventor 徐一达
Owner 徐一达
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