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A kind of extracellular matrix material with high biological activity and its preparation method and application

A biologically active, extracellular matrix technology, applied in the field of highly biologically active extracellular matrix materials and their preparation, can solve the problems of cumbersome operation in the oscillation process, and achieve the goal of retaining the content of biologically active components, good mechanical strength, and complete decellularization Effect

Active Publication Date: 2021-04-23
EXCELLENCE MEDICAL TECH SUZHOU CO LTD +2
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The operation of the oscillation process is cumbersome

Method used

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  • A kind of extracellular matrix material with high biological activity and its preparation method and application
  • A kind of extracellular matrix material with high biological activity and its preparation method and application
  • A kind of extracellular matrix material with high biological activity and its preparation method and application

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Embodiment 1

[0048] Raw material acquisition: The jejunum was taken out half an hour after the death of large pigs with plant-sourced feed closed-feeding, weighing more than 150 kg. After intracavitary flushing, place in the Changzheng-1 organ preservation solution at 2°C, and keep the temperature below 4°C during transportation.

[0049] Pre-sterilization: the porcine jejunum is connected to the perfusion reaction rod, and the cavity is perfused with 0.1% peracetic acid / 4% ethanol mixed disinfectant solution at a rate of 100ml / min / m, while the cavity is soaked in 0.1% peracetic acid / 4% Ethanol mixed with disinfectant, the treatment time is 5 minutes. It has been confirmed that porcine capsular virus can be completely inactivated after treatment for 5 minutes, the bioburden is reduced to no more than 2CFU / g, the amount of endotoxin is reduced to no more than 20EU / g, and the amount of indicated virus is reduced to 100PFU / g. No more than 10% loss in one step.

[0050] Separation of porcine...

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Abstract

The invention relates to a highly biologically active extracellular matrix material and its preparation method and application. The raw material is prepared by combined use of ultra-high pressure and perfusion inside and outside the cavity. The preparation method mainly includes: acquisition and preparation of raw materials, pre-treatment; mechanical separation of extracellular matrix; ultra-high pressure crushing and decellularization; washing out cell contents and removing antigens; final sterilization and preservation. Compared with the extracellular matrix obtained by decellularization by mechanical oscillation method, chemical reagent or biological reagent method, the present invention significantly improves the concentration of cohesin, growth factor, proteoglycan, glycoprotein, glycosaminoglycan, etc. The retention of biologically active components; at the same time, the mechanical strength is well preserved, the decellularization is thorough, and the biological load such as viruses is completely removed; the industrial production is convenient.

Description

technical field [0001] The invention belongs to the field of biological scaffold materials, in particular to a high biological activity extracellular matrix material and its preparation method and application. Background technique [0002] The extracellular matrix (ECM) component biological patch prepared by decellularization technology is an important development direction of soft tissue repair materials. Related products are widely used in various wounds in foreign countries, such as replacing meninges, pleura, blood vessels, periosteum, pericardium, joint capsule, etc., filling hemostasis when liver and spleen and other solid organs are damaged, strengthening anastomosis, bladder suspension, and pelvic floor reconstruction And the treatment of various complex hernias and abdominal wall defects, such as abdominal wall defects with contamination, infection after implantation of synthetic mesh, secondary surgical treatment of intestinal fistula, prevention of parastomal hern...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/58
CPCA61L27/3633A61L27/3687A61L27/3691A61L27/58A61L2430/40
Inventor 张剑
Owner EXCELLENCE MEDICAL TECH SUZHOU CO LTD
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