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Application of apigenin in preparation of medicine for preventing and treating hemorrhagic cerebrovascular disease and ischemic cerebrovascular disease caused by high blood pressure

A technology for cerebrovascular diseases and cerebrovascular diseases, applied in cardiovascular system diseases, drug combinations, antipyretics, etc., can solve the problems of clinical application and immaturity of drugs without cerebral hemorrhage, and achieve the promotion of cerebrovascular smooth muscle cell proliferation , Inhibition of cerebrovascular inflammatory response and enhancement of transcriptional activity

Inactive Publication Date: 2018-03-23
FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on the pathogenesis of hypertensive intracerebral hemorrhage is still immature, and no drugs for the prevention of intracerebral hemorrhage have been put into clinical application

Method used

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  • Application of apigenin in preparation of medicine for preventing and treating hemorrhagic cerebrovascular disease and ischemic cerebrovascular disease caused by high blood pressure
  • Application of apigenin in preparation of medicine for preventing and treating hemorrhagic cerebrovascular disease and ischemic cerebrovascular disease caused by high blood pressure
  • Application of apigenin in preparation of medicine for preventing and treating hemorrhagic cerebrovascular disease and ischemic cerebrovascular disease caused by high blood pressure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Combined application of extracranial and extracranial blood vessel expression profile database and network pharmacology research method to screen out that apigenin may have the effect of preventing and treating hypertensive cerebral hemorrhage

[0033] 1. Materials and Instruments

[0034] In this section, bioinformatics methods are used for drug prediction, and experimental materials and instruments are not involved.

[0035] 2. Method

[0036] 2.1 Selection of differential expression database of extracranial and intracranial blood vessels

[0037] Table 1 Differential expression database of extracranial and intracranial blood vessels

[0038]

[0039] 2.2 Data processing flow and differential gene screening

[0040] Such as figure 1 Shown is the process of expression profile chip data processing and differential gene screening. Download the CEL data (data files with the suffix CEL) from the GEO database, use R language to process the Oligo data pack...

Embodiment 2

[0048] Example 2: Apigenin significantly reduces the incidence and death risk of hypertensive cerebral hemorrhage in mice

[0049] 1. Materials and Instruments

[0050] Table 2 Materials and instruments required for preparation and evaluation of mouse intracerebral hemorrhage model

[0051] name

company

Specifications

ALZET Micro Osmotic Pump

DURECT

2006

AngII

Sigma

50mg

L-NAME

Sigma

25g

Apigenin

Select

S2262, 200 mg

Isoflurane

Huo Ning

100mL

small animal gas anesthetic

Vetequip

RC2 single channel

Small animal MRI imaging system

Brooke

ClinScan 7T

Small animal non-invasive blood pressure monitor

Softron

2010A

normal saline

Shijiazhuang Four Medicines

500mL

10% Paraformaldehyde

Shanghai Gefan Biology

500mL

PBS

GE

500mL

[0052] 2. Method

[0053] 2.1 Experimental animals

[0054] The experimenta...

Embodiment 3

[0074] Example 3: Sequencing of mouse cerebrovascular transcriptome further reveals the molecular mechanism of apigenin in protecting hypertensive cerebral hemorrhage

[0075] 1. Materials and Instruments

[0076] Table 4 Materials and instruments for isolation of mouse cerebral blood vessels, extraction of total RNA, and transcriptome sequencing

[0077]

[0078]

[0079] 2. Method

[0080] 2.1 Separation and extraction of mouse cerebral blood vessels

[0081] 2.1.1 Prepare sucrose buffer: 0.32mol / L sucrose (MW=342.3), 3mmol / L HEPES (MW=238.3), pH 7.4. 5.48g sucrose and 0.0357g HEPES are needed to configure 50mL buffer solution, prepared with DEPC water, and finally adjusted the pH value, and stored in a refrigerator at 4°C.

[0082] 2.1.2 Completely separate the mouse brain tissue, roll the brain carefully on the filter paper, remove the pia mater and choroid plexus, and wash once in sucrose buffer.

[0083] 2.1.3 Place the brain tissue in 5 mL of buffer solution, ...

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Abstract

The invention provides application of apigenin in the preparation of a medicine for preventing and treating hemorrhagic cerebrovascular disease and ischemic cerebrovascular disease caused by high blood pressure. The invention indicates that apigenin treatment can decrease the death rate of model mice with hypertensive cerebral hemorrhage by a dosage-dependent method. The apigenin decreases the onset risk of hypertensive cerebral hemorrhage by regulating the genetic expression of cerebrovascular smooth muscle cells.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of natural medicine apigenin in the preparation of medicines for preventing and treating hemorrhagic cerebrovascular disease and ischemic cerebrovascular disease caused by hypertension. Background technique [0002] Hypertensive disease can cause secondary damage to many organs, among which cerebrovascular disease caused by hypertension is the most serious type of complication and has become a major health burden for human beings. According to the "China Cardiovascular Disease Report 2016", there are at least 7 million stroke patients in China, and it is speculated that at least 20% of them are hemorrhagic strokes (ie, hemorrhagic cerebrovascular disease, cerebral hemorrhage), that is, at least 1.4 million, and the rest are ischemic strokes ( ischemic cerebrovascular disease). The "China Stroke Prevention Report (2015)" pointed out that stroke is the first cau...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P9/12A61P9/10A61P29/00
CPCA61K31/352
Inventor 陈敬洲崔庆华李昊孙莹莹张银辉宋莉陶瑾刘天龙肖宁徐昊晨
Owner FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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