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Method for detecting amino acid content in calcium zinc gluconate oral solution

A technology of calcium zinc gluconate, oral solution, applied in measuring devices, instruments, scientific instruments, etc., can solve the problem of not attracting enough attention from everyone, and achieve stable and reliable peak time, low detection cost, and good repeatability. Effect

Active Publication Date: 2018-03-20
HUNAN QIANJIN XIELI PHARMA CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This shows that the detection of related substances in calcium zinc gluconate oral solution has not attracted enough attention

Method used

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  • Method for detecting amino acid content in calcium zinc gluconate oral solution
  • Method for detecting amino acid content in calcium zinc gluconate oral solution
  • Method for detecting amino acid content in calcium zinc gluconate oral solution

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Embodiment

[0046] A kind of detection method of amino acid content in calcium zinc gluconate oral solution, this detection method adopts pre-column derivation-reversed phase high performance liquid chromatography, adopts 2,4-dinitrofluorobenzene as derivatization reagent, detection method comprises the following steps :

[0047] (1) Prepare the reference substance stock solution: Accurately weigh 15 mg each of arginine, threonine, proline, phenylalanine, leucine, and isoleucine into 25 mL volumetric flasks, dilute with water to To the mark, as arginine reference substance stock solution, threonine reference substance stock solution, proline reference substance stock solution, phenylalanine reference substance stock solution, leucine reference substance stock solution and isoleucine Acid reference substance stock solution (the concentration of each reference substance stock solution is 0.6mg / mL);

[0048] (2) Prepare the separation stock solution: Weigh about 120mg of lysine hydrochlorid...

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Abstract

The invention discloses a method for detecting amino acid content in calcium zinc gluconate oral solution. According to the detection method, pre-column derivatization-reversed-phase high-performanceliquid chromatography is adopted, and 2,4-dinitrofluorobenzene is taken as a derivatization reagent. The detection method comprises the following steps: (1) preparing a separation degree stock solution containing lysine hydrochloride and other impurity amino acids; (2) determining a corresponding relation between the dose of the derivatization reagent and the amino acid content in the separation degree stock solution; (3) preparing a test stock solution by utilizing calcium zinc gluconate oral solution, and carrying out derivation reaction, wherein the addition amount of the derivatization reagent is determined according to the corresponding relation determined in the step (2) and the amino acid content in the test stock solution; and (4) detecting by utilizing a reversed-phase high-performance liquid chromatograph. According to the method for detecting amino acid content in calcium zinc gluconate oral solution disclosed by the invention, the addition amount of the derivatization reagent is optimized, the pH value of a mobile phase is optimized, the detection process is excellent in repeatability, and the method is high in sensitivity and stable and reliable in appearance time.

Description

technical field [0001] The invention belongs to the field of pharmaceutical component analysis, in particular to a method for detecting amino acid content in oral solutions. Background technique [0002] Calcium zinc gluconate oral solution is a compound preparation composed of calcium gluconate, zinc gluconate and lysine hydrochloride. Manufacturers producing this variety on the market include Aonuo (China) Pharmaceutical Co., Ltd., Hubei Wushi Pharmaceutical Co., Ltd., and Hubei Furen Jinshen Pharmaceutical Co., Ltd. Calcium gluconate participates in the formation of bones, the reconstruction of bone tissue after fractures, muscle contraction, nerve transmission, and blood coagulation inhibition, and can reduce the permeability of capillaries. Zinc gluconate is an important component of many enzymes in the body, which can promote growth and development, improve taste and so on. Lysine hydrochloride is one of the 8 essential amino acids for the human body, which can promo...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89
CPCG01N30/89
Inventor 何爽张泽昀曹瑞谭喜平
Owner HUNAN QIANJIN XIELI PHARMA CO LTD
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