Carbon-based bio-organic fertilizer and preparation method and application thereof
A bio-organic fertilizer and carbon-based technology, applied in the direction of organic fertilizer, application, nitrogen fertilizer, etc., can solve the problems that bio-organic fertilizer has not been reported, and achieve the goal of increasing soil water and fertilizer retention, promoting crop growth, and strong colonization ability Effect
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Embodiment 1
[0035] Antagonistic effect of embodiment 1 functional microbial strains on Fusarium wilt pathogen
[0036] Pathogens tested: Phytophthora, Fusarium wilt pathogens;
[0037] Functional microorganisms: Bacillus amyloliquefaciens obtained by the applicant in the diseased soil, which was preserved in the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microorganisms on May 9, 2016, address: Beichen, Chaoyang District, Beijing No. 3, No. 1 Yard, West Road, Institute of Microbiology, Chinese Academy of Sciences, Zip Code 100101; strain preservation number: CGMCC No.12419, named by the applicant as Bacillus amyloliquefaciens L3;
[0038] Test medium: PDA medium: potato 200g / L (boil 200g potato for 20min, make up to 1L), glucose 20g / L, agar 15-20g / L;
[0039] Confrontation step: the pathogenic bacteria cultivated by the PDA medium is prepared into a 5mm diameter bacterial sheet, transferred to the center of another PDA plate, and the point of Bacillus...
Embodiment 2
[0042] The prevention and control effect of functional microbial bacterial liquid to pepper blight under the condition of embodiment 2 potted plants
[0043] The test crop is hot pepper, and the variety is Sujiao No. 5.
[0044] Pathogen tested: Phytophthora capsici
[0045] Functional microorganism: Bacillus amyloliquefaciens L3
[0046] Control group: no functional microbial bacterial solution (adding the same amount of sterile water as the test group).
[0047] Test group: application of functional microbial liquid, the number of functional bacteria in the potting soil is 10 7 pcs / g.
[0048] There were 15 pots in each of the control group and the test group.
[0049] The preparation method of the functional microorganism liquid: use the LB solid medium to activate the preserved species of Bacillus amyloliquefaciens strain L3, transfer it to the Erlenmeyer flask containing the LB liquid medium, and shake it at 30°C and 170r / min for 24h. Centrifuge at 7000r / min for 10mi...
Embodiment 3
[0055] Embodiment 3 prepares charcoal-based bio-organic fertilizer
[0056] Taking the production of 1000kg carbon-based bio-organic fertilizer as an example, the specific steps are as follows:
[0057] Preparation of biochar: dry the rice straw to a water content below 20%, then crush it to a length of <5cm, put it into a carbonization furnace at 500°C, and after 2 hours of carbonization, remove the charcoal and crush it to 60 mesh to obtain biochar.
[0058] Preparation of pig manure and straw compost: add straw to pig manure to adjust the carbon-nitrogen ratio to 25, adjust the water content to 60%, and carry out high-temperature aerobic fermentation. When the temperature is lower than 50°C, the stacking is stopped, and it is prepared after stacking and fermenting for 60 days.
[0059] Preparation of functional microbial fermentation broth: Inoculate the functional microbial strain (Bacillus amyloliquefaciens L3) stored at 4°C on a beef peptone plate, culture at 30°C for 4...
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