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Detection method and detection apparatus of analysis detection test paper data

A technology for testing test strips and testing devices, which is used in measuring devices, analyzing materials, and analyzing materials through chemical reactions, etc., can solve cross-infection, rarely consider temperature, fatigue and other problems, to ensure accuracy, expand Effects of using range and sensitivity

Pending Publication Date: 2018-03-13
北京安百胜诊断科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, early detection of ectopic pregnancy is of great help to protect female reproductive function and prevent habitual abortion. However, preventive testing of ectopic pregnancy requires repeated measurement of HCG concentration. If HCG concentration doubles every 48 hours, it is inferred to be normal Intrauterine pregnancy, if the concentration is significantly lower than double, the possibility of ectopic pregnancy or dysplasia will increase, so pregnant women need to go to the hospital for HCG concentration testing many times, which brings a lot of fatigue and the risk of cross-infection in the hospital
However, the existing detection test strips are still unable to be tested at home, which is limited by the limitation of home quantitative detection technology
[0005] Even the test papers in professional institutions rarely consider the influence of temperature on the test results. The present invention finds that under different temperature conditions, the test results will have large differences, so the temperature will seriously affect the accuracy of the test results.

Method used

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  • Detection method and detection apparatus of analysis detection test paper data
  • Detection method and detection apparatus of analysis detection test paper data
  • Detection method and detection apparatus of analysis detection test paper data

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1: Detection of heart-type fatty acid binding protein at different temperatures

[0075] Heart-type fatty acid binding protein (hFABP) quantitative detection kit (batch number: 20160602) was used to react and detect in the environment of 10°C, 25°C, and 45°C respectively, and the concentrations of the test samples were 150ng / mL, 37.5ng / mL, 9.375ng / mL, 4.69ng / mL, 2.34 each concentration point detection 2 parallel cards, calculate the T / C mean. The test results are shown in Table 1.

[0076] Table 1 T / C value of detecting hFABP under different temperatures and concentrations

[0077] hFABP concentrationng / ml

10℃

25℃

45℃

150.00

1.159

1.303

1.603

37.50

0.575

0.876

1.236

9.375

0.287

0.634

0.634

4.69

0.127

0.206

0.256

2.34

0.071

0.108

0.148

0

0.002

0.065

0.075

[0078] It can be seen from the above table that temperature has a great influence on the T / C v...

Embodiment 2

[0087] Example 2: Detection of procalcitonin at different temperatures

[0088] Procalcitonin (PCT) Quantitative Detection Kit (batch number: 20151230) was used to react and detect in the environment of 10°C, 25°C, and 45°C respectively, and the concentrations of the test samples were 50ng / mL, 25ng / mL, 5ng / mL, 2ng / mL, 1ng / mL, 0ng / mL, detect 2 parallel cards at each concentration point, and calculate the mean T / C value. The test results are shown in Table 2.

[0089] Table 2 T / C value of PCT under different temperatures and concentrations

[0090]

[0091] According to table 2 result, take concentration ng / mL as x-axis, T / C value is y-axis plotting, obtain the reaction curve of PCT under different temperature conditions, see Figure 6.

[0092] From the figure, we can see the influence of temperature on the T / C value. Select the line graph where the temperature is 10°C, and perform four-parameter Logistic curve fitting to obtain the curve equation.

[0093] Equation: y=...

Embodiment 3

[0100] Example 3: Detection of myoglobin at different temperatures

[0101] The myoglobin (Myo) quantitative detection kit (batch number: 20160505) was used to react and detect in the environment of 10°C, 25°C, and 40°C respectively, and the concentrations of the test samples were 500ng / mL, 250ng / mL, and 125ng / mL. , 50ng / mL, 1ng / mL, detect 2 parallel cards at each concentration point, and calculate the T / C mean value. The test results are shown in Table 3.

[0102] Table 3 T / C value of detecting Myo under different temperatures and concentrations

[0103] Myo concentrationng / ml

10℃

25℃

40℃

1.00

0.1486

0.4071

0.3155

50.00

0.2443

0.6659

1.2039

125.00

0.5708

1.5077

2.4140

250.00

1.3417

3.0167

4.0051

500.00

2.0650

4.4332

6.1359

[0104] According to table 3 result, take concentration ng / mL as x-axis, T / C value is y-axis plotting, obtain the reaction curve of Myo under different temp...

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PUM

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Abstract

The invention discloses a calibration method and detection apparatus of a detection reagent. The calibration method is used to calibrate the concentration of a sample to be detected. The detection apparatus calculates the signal values obtained from the test area and control area of test paper so as to obtain the concentration of the sample.

Description

technical field [0001] The invention belongs to the field of test paper reading and analysis, in particular to a test paper concentration detection method and detection device based on temperature calibration. Background technique [0002] Test strips have been widely used in sample testing in many fields, such as routine urine testing, prenatal and postnatal care, and infertility testing commonly used in clinical medicine, formaldehyde and benzene content testing in environmental testing, and food safety testing. Clenbuterol, melamine, waste oil or pesticide residue detection, etc., need to use the corresponding special test paper for detection. [0003] Some simple test paper tests can be achieved by visually observing the test paper, such as early pregnancy test paper, but this method of visual test paper can only be used for qualitative detection, and if the content of the detected object is low and the color is light , It is prone to misreading, and the naked eye obser...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N33/558
CPCG01N21/78G01N33/558
Inventor 张磊程海邓双胜夏丽侯得志王里奇贺率宋小力刘勇
Owner 北京安百胜诊断科技有限公司
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